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研究生:郭家愷
研究生(外文):KUO, CHIA-KAI
論文名稱:中藥精油萃取效率及促進經皮吸收與抗菌功效評估
論文名稱(外文):Evaluation of Extraction Efficiency of Traditional Chinese Medicine Essential Oil, Promotion of Percutaneous Absorption and Antibacterial Efficacy
指導教授:李佳穎李佳穎引用關係
指導教授(外文):LI, CHIA-YING
口試委員:施焜燿詹于誼
口試委員(外文):SHIH, KUN-YAUHCHAN, YU-YI
口試日期:2019-06-26
學位類別:碩士
校院名稱:國立屏東大學
系所名稱:應用化學系碩士班
學門:自然科學學門
學類:化學學類
論文種類:學術論文
論文出版年:2019
畢業學年度:107
語文別:中文
論文頁數:80
中文關鍵詞:小茴香白荳蔻經皮穿透抗菌能力
外文關鍵詞:Fennelcardamomskin penetrationantibacterial ability
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本實驗利用頂空固相微萃取法結合氣象層析質譜分析作為分析小茴香與白荳蔻香氣成分的基準。評估水蒸餾法、水蒸氣蒸餾法及索氏萃取法的萃取差異及效率。另進行此兩種精油對維他命C及Methylparaben之經皮促進效果。此外,也進行小茴香和白荳蔻精油的抗菌測試。
其中索氏萃取法與蒸餾法有很大的差異。除了揮發性成分外,還多了溶劑反應產物和低極性高沸點之植物油脂或脂肪酸。在促進經皮穿透試驗中,添加小茴香精油的實驗組,維他命C的穿透明顯受到抑制,而對Methylparaben的穿透無影響。添加白荳蔻精油的實驗組,維他命C的穿透同樣受到抑制,而Methylparaben的穿透則隨時間而有增加的現象。顯示精油確實會對某些成分的經皮吸收造成影響。另外,小茴香和白荳蔻精油皆具有良好的抗菌效果,可做為化妝保養品之替代防腐系統。
In this experiment, headspace solid-phase microextraction combined with gas chromatography-mass spectrometry was used as the benchmark for the analysis of the aroma components of fennel and cardamom. We compare the extraction difference and efficiency between water distillation, steam distillation and Soxhlet extraction method. Percutaneous penetration effects of these two essential oils on vitamin C and methylparaben were also performed. In addition, fennel and cardamom essential oils were tested for their antibacterial ability.

The results of Soxhlet extraction is quite different from distillation method. In addition to the volatile components, there are more solvent reaction products and low-polarity, high-boiling vegetable oils or fatty acids in Soxhlet extraction method. The experimental group which added of fennel essential oil was significantly inhibited the percutaneous penetration of vitamin C. In the promotion of percutaneous penetration of methylparaben, the experimental group which added of fennel essential oil was no effect on methylparaben. In the test group added cardamom essential oil, the penetration of vitamin C was also inhibited, while the penetration of methylparaben increased with time. This shows that essential oils have an effect on percutaneous absorption of certain ingredients. In addition, fennel and cardamom essential oils have good antibacterial effects and can be used as an alternative anti-corrosion system for cosmetics and care products.

誌謝-------------------------------------------------------------------------Ⅰ
中文摘要-------------------------------------------------------------------Ⅱ
Abstract--------------------------------------------------------------------Ⅲ
目錄-------------------------------------------------------------------------Ⅳ
第一章 緒論
第一節 前言----------------------------------------------------------------1
第二節 精油萃取方法----------------------------------------------------4
第三節 經皮穿透系統簡介----------------------------------------------6
第四節 抗菌活性----------------------------------------------------------10
第五節 研究動機與目的-------------------------------------------------11
第二章 文獻探討
第一節 植物介紹-----------------------------------------------------------12
第二節 精油萃取-----------------------------------------------------------15
第三節 成分分析-----------------------------------------------------------16
第四節 精油應用-----------------------------------------------------------22
第三章 材料與方法
第一節 儀器與材料--------------------------------------------------------24
第二節 萃取方法-----------------------------------------------------------26
第三節 成分分析-----------------------------------------------------------27
第四節 經皮促進測試-----------------------------------------------------28
第五節 抗菌活性-----------------------------------------------------------30
第四章 結果與討論
第一節 各種萃取方法之精油的成分探討----------------------------31
第二節 精油促進經皮穿透能力探討----------------------------------63
第三節 抗菌活性-----------------------------------------------------------71
第五章 結論-----------------------------------------------------------------73
參考文獻---------------------------------------------------------------------74
附錄----------------------------------------------------------------------------81

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