臺灣博碩士論文加值系統

目的：探討補充大豆分離蛋白(Isolated soy protein, ISP)合併有氧運動訓練
(Aerobic exercise, AE)對於身體組成和運動能力表現之影響。方法：本實驗使用
六週齡、雄性ICR品系小鼠，隨機分做四組(每組n=8)：(1)控制組(Control)、(2)大
豆分離蛋白組(1.5 mg/kg/day, ISP)、(3)有氧運動組(AE)、(4)有氧運動合併大豆分
離蛋白組(ISP+AE)。有氧運動訓練與ISP經管餵食6週後。隨後進行下列測試，項
目包括：前肢抓力(爆發力測試)、 5%負重游泳力竭時間(有氧耐力測試)、單次
15分鐘運動挑戰後血液中疲勞指數(血乳酸)與肌肉損傷指標(Creatine Kinase,
CK)之變化，測試期間能繼續補充ISP。所有數據以SAS統計軟體進行雙因子變異
數分析，並以p<0.05表示具有統計上的意義。結果：在運動表現方面，游泳力竭
時間在ISP+AE顯著比Control提升(P<0.05)。運動後15分鐘的血液生化部分，血乳
酸濃度、骨骼肌損傷生化指標CK活性皆有顯著地下降(P<0.05)。結果：6週的AE
模式下補充ISP，顯著增加在抓力測試和游泳運動能力表現上；且在15分鐘測試
後的血液生化表現部分，Lactace、NH3和CK活性顯著下降，在臨床血液生化值
的部分，天門冬胺酸轉胺酶(AST)、總膽固醇(TC)、三酸甘油脂(TG)、肌酸酐
(CREA)均顯著下降。結論：本研究證實有氧運動訓練合併補充大豆分離蛋白，
比單獨訓練更能提升生物體運動能力表現和身體組成之功效。

Purpose: In this study, we designed an aerobic exercise training (AE) program
combine Isolated Soy Protein (ISP) with a mouse model based on a body composition
and exercise performance. Methods: Male ICR mice were divided into 4 groups (n =
8 per group) for 6-weeks treatment: Control, ISP, AE and AE groups with ISP
supplementation (AE+ISP). Exercise performance was evaluated by forelimb grip
strength and exhaustive swimming time as well as changes in body composition and
anti-fatigue activity levels of serum lactate, NH3, glucose, and creatine kinase (CK) ,
Lactace after a 15-min swimming exercise. The biochemical parameters were
measured at the end of the experiment. Results: 6-week AE+ISP significantly
increased grip strength and exhaustive swimming time. AE+ISP supplementation
significantly decreased serum lactate, ammonia and CK levels after 15-min swimming
exercise. The resting serum levels of AST, TG , TC and CREA were all significantly
decreased with AE+ISP. Conclusion: This study confirmed that aerobic exercise
combined with soy protein isolate, than the individual training to enhance the
performance of the body's athletic ability and body composition of the effectiveness.

目 錄
中文摘要........................................................................................................................I
英文摘要.......................................................................................................................II
致謝….........................................................................................................................III
目錄.............................................................................................................................IV
表目錄.........................................................................................................................VI
圖目錄........................................................................................................................... VIII
第壹章 緒論 ........................................................................................................ 1
第一節 研究背景 ............................................................................................... 1
第二節 研究目的 ............................................................................................... 2
第貳章 文獻探討 ....................................................................... 3
第一節 有氧運動介紹 ....................................................................................... 3
第二節 大豆分離蛋白介紹 ............................................................................... 5
第三節 支鏈胺基酸對運動之功效 ................................................................... 6
第四節 文獻小節 ............................................................................................... 8
第參章 材料與方法 ................................................................... 9
第一節 實驗測試樣品 ....................................................................................... 9
第二節 實驗動物之飼養及分組 ..................................................................... 12
第三節 實驗內容與方法 ................................................................................. 13
第四節 血液與組織樣本之收集及前處理 ..................................................... 20
第五節 統計分析 ............................................................................................. 22
第肆章 結果與討論 .................................................................. 23

第一節 實驗期間各組動物體重、組織器官重量與飲食之變化情形 ......... 23
第二節 前肢抓力表現 ..................................................................................... 27
第三節 游泳力竭運動能力表現 ..................................................................... 28
第四節 15 分鐘運動挑戰後血液生化變化之情形 ........................................ 29
第五節 組織中肝醣含量 ................................................................................. 32
第六節 補充大豆分離蛋白合併有氧運動訓練對於臨床血液生化之影響 . 34
第七節 組織病理切片觀察結果 ..................................................................... 36
第伍章 結論 .............................................................................. 38
參考文獻 .................................................................................................................. 39

參考文獻
Agha, F. E., Youness, E. R., Selim, M. M., & Ahmed, H. H. (2014). Nephroprotective
potential of selenium and taurine against mercuric chloride induced nephropathy
in rats. Ren Fail. Jun, 36(5), 704-16.
Ahmed, M. S., Calabria, A. C., & Kirsztajn, G. M. (2011). Short-term effects of soy
protein diet in patients with proteinuric glomerulopathies. J Bras Nefrol, 33(2),
150-159.
Anderson, J. W., Blake, J. E., Turner, J., & Smith, B. M. (1998). Effects of soy protein
on renal function and proteinuria in patients with type 2 diabetes. The American
journal of clinical nutrition, 68(6), 1347S-1353S.
Blomstrand, E. (2006). A role for branched-chain amino acids in reducing central
fatigue. The Journal of nutrition, 136(2), 544S-547S.
Blomstrand, E., & Newsholme, E. A. (1992). Effect of branched‐chain amino acid
supplementation on the exercise‐induced change in aromatic amino acid
concentration in human muscle. Acta Physiologica, 146(3), 293-298.
Blomstrand, E., Hassmén, P., Ek, S., Ekblom, B., & Newsholme, E. A. (1997).
Influence of ingesting a solution of branched‐chain amino acids on perceived
exertion during exercise. Acta Physiologica Scandinavica, 159(1), 41-49.
Chuang, H. L., Huang, Y. T., Chiu, C. C., Liao, C. D., Hsu, F. L., Huang, C. C., & Hou,
C. C. (2012). Metabolomics characterization of energy metabolism reveals
glycogen accumulation in gut-microbiota-lacking mice. The Journal of nutritional
biochemistry, 23(7), 752-758.
40
Chen, W. C., Huang, W. C., Chiu, C. C., Chang, Y. K., & Huang, C. C. (2014). Whey
protein improves exercise performance and biochemical profiles in trained mice.
Med. Sci. Sports Exerc, 46, 1517-1524.
Elia, D., Stadler, K., Horváth, V., & Jakus, J. (2006). Effect of soy-and whey proteinisolate
supplemented diet on the redox parameters of trained mice. European
journal of nutrition, 45(5), 259-266.
Huang, C. C., Hsu, M. C., Huang, W. C., Yang, H. R., & Hou, C. C. (2012). Triterpenoidrich
extract from Antrodia camphorata improves physical fatigue and exercise
performance in mice. Evidence-Based Complementary and Alternative Medicine,
2012.
van Loon, L. J., & Goodpaster, B. H. (2006). Increased intramuscular lipid storage in
the insulin-resistant and endurance-trained state. Pflügers Archiv, 451(5), 606-
616.
Wang, J. J., Shieh, M. J., Kuo, S. L., Lee, C. L., & Pan, T. M. (2006). Effect of red
mold rice on antifatigue and exercise-related changes in lipid peroxidation in
endurance exercise. Applied microbiology and biotechnology, 70(2), 247-253.
Margolis, L. M., & Pasiakos, S. M. (2013). Optimizing intramuscular adaptations to
aerobic exercise: effects of carbohydrate restriction and protein supplementation
on mitochondrial biogenesis. Advances in Nutrition: An International Review
Journal, 4(6), 657-664.
Mero, A. (1999). Leucine supplementation and intensive training. SPORTS
MEDICINE-AUCKLAND-, 27, 347-358.
41
Michael J. Ormsbee , Christopher W. Bach and Daniel A. Baur.(2014). Pre-Exercise
Nutrition: The Role of Macronutrients, Modified Starches and Supplements on
Metabolism and Endurance Performance. Nutrients , 6(5), 1782-1808
Newsholme EA, Blomstrand E, Ekblom B: Physical and mental fatigue: metabolic
mechanisms and importance of plasma amino acids. Br Med Bull. 1992, 48 (3):
477-495.
Ormsbee, M. J., Bach, C. W., & Baur, D. A. (2014). Pre-exercise nutrition: the role of
macronutrients, modified starches and supplements on metabolism and endurance
performance. Nutrients, 6(5), 1782-1808.
Pipe, E. A., Gobert, C. P., Capes, S. E., Darlington, G. A., Lampe, J. W., & Duncan, A.
M. (2009). Soy protein reduces serum LDL cholesterol and the LDL
cholesterol:HDL cholesterol and apolipoprotein B:apolipoprotein A-I ratios in
adults with type 2 diabetes. J Nutr, 139(9), 1700-1706.
Ryan-Borchers, T. A., Park, J. S., Chew, B. P., McGuire, M. K., Fournier, L. R., &
Beerman, K. A. (2006). Soy isoflavones modulate immune function in healthy
postmenopausal women. Am J Clin Nutr, 83(5), 1118-1125.
Su KY, Yu CY, Chen YW, Huang YT, Chen CT, Wu HF, & Chen YL (2014). Rutin, a
flavonoid and principal component of saussurea involucrata, attenuates physical
fatigue in a forced swimming mouse model. Int J Med Sci, 11(5), 528-537.
Torre-Villalvazo, I., Gonzalez, F., Aguilar-Salinas, C. A., Tovar, A. R., & Torres, N.
(2009). Dietary soy protein reduces cardiac lipid accumulation and the ceramide
concentration in high-fat diet-fed rats and ob/ob mice. The journal of Nutrition,
139(12), 2237-2243.
42
Warren, G. L., Ingalls, C. P., Lowe, D. A., & Armstrong, R. B. (2001). Excitationcontraction
uncoupling: major role in contraction-induced muscle injury.
Exercise and sport sciences reviews, 29(2), 82-87.
Wang, S. Y., Huang, W. C., Liu, C. C., Wang, M. F., Ho, C. S., Huang, W. P., Hou, C.
C., Chuang, H. L., & Huang, C. C. (2012). Pumpkin (Cucurbita moschata) fruit
extract improves physical fatigue and exercise performance in mice. Molecules,
17(10), 11864-11876.
Wu RE, Huang WC, Liao CC, Chang YK, Kan NW., & Huang CC (2013). Resveratrol
protects against physical fatigue and improves exercise performance in mice.
Molecules, 18(4), 4689-4702.
Wagenmakers, A. J., Beckers, E. J., Brouns, F. R. E. D., Kuipers, H. A. R. M.,
Soeters, P. B., Van Der Vusse, G. J., & Saris, W. H. (1991). Carbohydrate
supplementation, glycogen depletion, and amino acid metabolism during
exercise. American Journal of Physiology-Endocrinology And Metabolism,
260(6), E883-E890.
Yamamoto, T. & Newsholme, E. A. (2000). Diminished central fatigue by inhibition
of the L-system transporter for the uptake of tryptophan. Brain Research
Bulletin,52(1), 35-38.
Yeh TS, Chuang HL, Huang WC, Chen YM, Huang CC, & Hsu M C (2014) Astragalus
membranaceus Improves Exercise Performance and Ameliorates Exercise-Induced
Fatigue in Trained Mice. Molecules, 19(3), 2793-2807.
Yu, B., Lu, Z. X., Bie, X. M., Lu, F. X., & Huang, X. Q. (2008). Scavenging and antifatigue
activity of fermented defatted soybean peptides. European Food Research
and Technology, 226(3), 415-42