臺灣博碩士論文加值系統

本研究探討橋腦側蓋區（lateral tegmental field,FTL）與巨大細胞核被蓋區（gigantocellular tegmental field,FTG）對心臟血管的調控機制所扮演的角色。上述二核區在血壓的調控上與延腦尾端腹外側區（caudal ventrolateral medulla,CVLM）的關連性。以及FTG的心跳下降作用與疑核（ambiguus nucleus,AN）及背側迷走運動神經核（dorsol motor nucleus of vagus,DMV）間的相互關係。同時使用快速傅立業轉換（Fast Fourier Transform,FFT）探討破壞CVLM、AN或DMV前後，分別刺激FTG及FTL，引發椎神經活性（vertebral nerve activity,VNA）及動脈壓（systemic arterial pressure,SAP）頻譜變化的情形，比較其頻譜特徵及生理意義。
本實驗以65隻貓為實驗動物，以尿酯（urethane,400mg/kg）及氯醛醣（α-chloralose,40mg/kg）的混合試劑來進行腹腔麻醉，記錄動脈血壓、心跳及交感神經系統的椎神經活性。利用電流（80Hz,50∼100uA），及化學物質麩胺酸鈉（sodium glutamate,Glu,0.25M,70nl），刺激FTL、FTG、CVLM、AN及DMV，再利用海人草酸（kainic acid,KA,24mM,150nl）破壞CVLM、AN及DMV。比較刺激FTL與FTG引起的血壓、心跳與交感神經反應，在CVLM、或AN、或DMV破壞前後，是否有改變。
實驗結果顯示，破壞CVLM前，以Glu刺激FTL，會造成血壓下降的反應，使用KA破壞CVLM後，再以Glu刺激FTL，則變成為血壓上升的反應。所以，FTL的血壓下降作用確實為透過CVLM作用所達成。破壞CVLM前，以Glu注入FTG，產生血壓上升，以KA破壞CVLM後，再以Glu注入FTG，則血壓上升的幅度變小。因此，FTG的血壓上升作用，應為透過抑制CVLM降壓機制所產生。FTG的心跳下降作用，由實驗結果顯示，破壞同側AN、或對側AN、或同側DMV後，再以Glu刺激FTG，皆不再能產生心跳下降。所以，FTG的心跳下降作用，是透過AN與DMV的調控作用，且AN的作用比DMV重要。頻譜分析的結果顯示，FTL與CVLM；FTG與CVLM；FTG與AN及FTG與DMV間確實有相互的關連性。

The aim of the present study is to examine the relationship between the lateral tegmental field (FTL), a depressor area, the gigantocellular tegmental field (FTG), a pressor area, and the depressor caudal ventrolateral medulla (CVLM) of the brain stem; and the relationship between FTG and two cardioinhibitory areas, i.e., the ambiguus nucleus (AN) and the dorsal motor nucleus of vagus (DMV). Fast fourier transform (FFT) was used to analyze the basal vertebral nerve activity (VNA) and the systemic arterial pressure (SAP) in frequency domain.
Sixty five cats were anesthetized intraperitoneally with chloralose (40mg/kg) and urethane (400mg/kg). Systemic arterial pressure (SAP), heart rate (HR) and the sympathetic vertebral nerve activity (VNA) were recorded. The correct location of FTL, FTG, CVLM, AN or DMV was determined first by electrical stimulation ( 80 Hz, 0.5 ms, 50 -100 uA ) then followed by microinjection of sodium glutamate (Glu, 0.25M, 70nl). It was found that the depressor responses caused by the FTL stimulation were greatly reduced after lesioning the CVLM by microinjection of kainic acid (KA, 24mM, 150nl) ipsilateral to the side of the FTL stimulation. Data suggest that the FTL-depressor responses probably are mediated through excitation of the depressor neurons in CVLM. The FTG pressor response were reduced after the CVLM was lesioned. Data suggest that the FTG-pressor response probably are mediated through inhibition of the neurons in CVLM. Lesioning the DMV or AN by KA reduced the FTG-induced bradycardiac responses. The reduction after the AN lesion ,however, was more pronounced than that after DMV lesion. Data suggest that part of the FTG bradycardiac action is mediated through activation of the AN or DMV.

中文摘要......................................................Ⅰ
英文摘要......................................................Ⅱ
縮寫表........................................................Ⅲ
誌謝..........................................................Ⅳ
目錄..........................................................Ⅴ
圖索引........................................................Ⅵ
表索引........................................................Ⅶ
第一章 緒論...................................................1
第一節 研究背景介紹............................................1
第二節 實驗目的................................................4
第三節 理論模式................................................5
第二章 材料與方法.............................................7
第一節 一般手術程序............................................7
第二節 交感神經活性的記錄......................................8
第三節 中樞神經系統的刺激.....................................10
第四節 電刺激及藥物施予.......................................11
第五節 實驗流程...............................................13
第六節 刺激點之確定...........................................14
第七節 資料統計與頻譜分析.....................................15
第三章 結果..................................................16
第一節 破壞CVLM對FTL調控血壓及心跳作用的影響..................16
第二節 破壞CVLM對FTG調控血壓及心跳作用的影響..................23
第三節 破壞AN對FTG調控心跳及血壓作用的影響....................30
第四節 破壞DMV對FTG調控心跳及血壓作用的影響...................37
第四章 討論..................................................44
第一節 FTL與CVLM的相關性......................................44
第二節 FTG與CVLM的相關性......................................45
第三節 FTG與AN的相關性........................................45
第四節 FTG與DMV的相關性.......................................46
第五節 椎神經放電活性的頻譜分析之探討.........................46
第五章 結論..................................................48
第六章 參考文獻..............................................49

1.Aicher, S.A., O.S. Kurucz, D.J. Reis and T.A. Milner Nucleus tractus solitarius efferent terminals synapes on neurons in the caudal ventrolateral medulla that project to the rostral ventrolateral medulla. Brain Res. 693: 51-63, 1995
2.Amatruda III, T.T., D.A. Black, T.M. McKenna, R.W. McCarley
and J.A. Hobson Sleep cycle control and cholinergic mechanisms:differential effects of carbachol injections at pontine brain stem sites.Brain Res. 98: 501-15, 1975
3.Barman, S.M. and G.L. Gebber Rostral ventrolateral medullary
and Caudal medullary raphe neurons with activity correlated to the 10-Hz rhythm in sympathetic nerve discharge. J. Neurophysiol. 68: 1535-47, 1992
4.Barman, S.M. and G.L. Gebber Lateral tegmental field neurons of cat medulla: a source of basal activity of ventrolateral medullospinal sympathoexcitatiry neurons. J. Neurophysiol. 57: 1410-24, 1987
5.Barman, S.M. and G.L. Gebber Lateral tegmental field neurons play a permissive role in governing the 10-Hz rhythm in sympathetic nerve discharge. Am. J. Physiol. 265: R1006-13, 1993
6.Barman, S.M., H.S. Orer and G.L. Gebber Caudal ventrolateral
medullary neurons are elements of the network responsible for the 10-Hz rhythm in sympathetic nerve discharge. J. Neurophysiol. 72: 106-20, 1994
7. Barman, S.M., H.S. Orer and G.L. Gebber A 10-Hz rhythm
reflects the organization of a brainstem network that specifically governs sympathetic nerve discharge. Brain Res. 671: 345-50, 1995
8.Blair, R.W., Response of feline medial medullary reticulospinal neurons to cardiac input. J. Neurophysiol. 58: 1149-67, 1987
9.Bonhan, A.C. and I. Jesje Cardiorespiratory effects of DL-
homocysteic acid in caudal ventrolateral medulla. Am. J. Physiol. 256: H688-96, 1989
10.Borison, H.L. and D. Domjan Persistence of the cardio inhibitory response to brain stem is chaemia after destruction of the area postrema and the dorsal vagal nuclei. J. Physiol. Lond. 211: 263-77, 1970
11.Calaresu, F.R., Medullary basal sympathetic tone. Ann. Rev.
Physiol. 50: 511-24, 1988
12.Chai, C.Y., S.Y. Chen, A. Lin and C.J. Tseng Angiotensin II activates pressor and depressor sites of the pontomedulla that react to glulamate. Clin. Exp. Pharmacol. Physiol. 23: 415-23, 1996
13.Chai, C.Y., S.Y. Chen, S.D. Wang, C.J. Tseng, R.H. Lin, S.P. Mao,H.T. Horng, J.C. Liu and J.S. Kuo Precollicular decerebration reduces the pressor responses evoked by stimulation of rostral pons but not medulla in cats. J. Auton. Nerv. Syst. 46: 147-59, 1993
14.Chan, S.H.H., B.T. Ong and P.T.H. Wong Suppression of arterial pressure, heart rate and cardiac contractility following microinjection of kainic acid into the nucleus ambiguus of the rat. Neurosci. Lett. 47: 57-62, 1984
15.Chen, S.Y., W.C. Wu, C.J. Tseng, J.S. Kuo and C.Y. Chai
Involvement of non-NMDA and NMDA peceptors in glutamate-
induced pressor or depressor responses of the pons and medulla. Clin.Exp. Pharmacol. Physiol. 24: 46-56, 1997
16.Chess, G.F., R.M.K. Tam and F.R. Calaresu Influence of cardiac neural inputs on rhythmic variations of heart period in the cat. Am. J. Physiol. 228(3): 775-80, 1975
17.Ciriello, J. and F.R. Calaresu Medullary origin of vagal
preganglionic axons to the heart of the cat. J. Auton. Nerv. Syst. 5: 9-22, 1982
18.Clement, M.E. and R.B. McCall Evidence that the lateral tegmental field plays an important role in the central sympathoinhibitory action of 8-OH-DPAT. Brain Res. 587: 115-22, 1992
19.Clement, M.E. and R.B. McCall Impairment of baroreceptor
reflexes following Kainic acid lesions of the lateral tegmental field. Brain Res. 618: 328-32, 1993
20.Coyle, J.T., M.E. Molliver and M.J. Kuhar In situ injection of kainic acid: a new method for selectively lesioning neuronal Cell bodies while sparing axons of passage. J. Comp. Neurol. 180: 301-24, 1978
21.Cravo, S.L. and S.F. Morrison The caudal ventrolateral medulla is a source of tonic sympathoinhibition. Brain Res. 621: 133-36, 1993
22.Cravo, S.L., S.F. Morrison and D.J. Reis Differentiation of two cardiovascular regions within caudal ventrolateral medulla. Am. J. Physiol. 261: R985-94, 1991
23.Dempesy, C.W., D.E. Richardson and C.J. Fontana
Sympathetically-mediated cardiovascular responses induced by
neurochemical microinjection in the brainstem lateral tegmental field of cat. Brain Res. 704: 141-44, 1995
24.Elisevich, K., A.W. Hrycyshyn and B.A. Flumerfelt
Supramedullary projections to the dorsal and ventrol divisions of the paramedian reticular nucleus in the cat. Exp. Brain Res. 58: 368-78, 1985
25.Elisevich, K.V., A.W. Hrycyshyn and B.A. Flumerfelt Cerebellar, medullary and spinal afferant connections of the paramedian reticular nucleus in the cat. Brain Res. 332: 267-82, 1985
26.Ellenberger, H.H. and J.L. Feldman Brainstem connections of the rostral ventral respiratory group of the rat. Brain Rse. 513: 35-42, 1990
27.Ellenberger, H.H., J.L. Feldman and W.Z. Zhan Subnuclear
organization of the lateral tegmental field of the rat. II: catecholamine neurons and ventral respiratory group. J. Comp. Neurol. 294: 212-22, 1990
28.Farkas, E., A.S.P. Jansen and A.D. Loewy Periaqueductal gray
matter input to cardiac-related sympathetic permotor neurons. Brain Res. 792: 179-92, 1998
29.Fateev, M.M. and A.D. Nozdrachev Projections of stellate ganglion sympathetic neutrons in cats. J. Auton. Nerv. Syst. 51: 129-34, 1995
30.Fonnum, F., Glutamate: a neurotransmitter in Mammalian Brain. J. Neurochem. 42: 1-11, 1984
31.Fung, M.L., Q. Huang, D. Zhou and W.M.S. John The
Morphology and connections of neurons in the gasping center of adult rats. Neuroscience 76: 1237-44, 1996
32.Gebber, G.L., Central oscillators responsible for sympathetic nerve discharge. Am. J. Physiol. 239: H143-55, 1980
33.Gebber, G.L. and S.M. Barman Brain stem neurons governing the discharge of sympathetic nerves. J. Auton. Nerv. Syst. 5: 55-61, 1982
34.Gebber, G.L. and S.M. Barman Lateral tegmental field neurons of cat medulla: a potential source of basal sympathetic nerve discharge. J. Neurophysiol. 54: 1498-512, 1985
35.Gerrits, P.O. and G. Holstege Pontine and medullary projections to the nucleus retroambiguus: a wheat germ agglutinin-horseradish peroxidase and autoradiographic tracing study in the cat. J. Comp. Neurol. 373: 173-85, 1996
36.Gilbert, K.A. and R. Lydic Pontine cholinergic reticular
mechanisms cause statedependent change in the discharge of
parabrachial neurons. Am. J. Physiol. 266: R136-50, 1994
37.Guyenet, P.G., N. Koshiya, D. Huangfu, S.C. Baraban, R.L.
Stornetta and Y.W. Li Role of medulla oblongata in generation of sympathetic and vagal outflows. Brain Res. 107: 127-44, 1996
38.Holstege, G. and J. Tan Projections from the red nucleus and
surrounding areas to the brainstem and spinal cord in the cat. an HRP and autoradiographical tracing study. Behav. Brain Res. 28: 33-57, 1988
39.Hsieh, J.H., R.F. Chen, J.J. Wu, C.T. Yen and C.Y. Chai Vagal innervation of the gastrointestinal tract arises from dorsal motor nucleus while that of the heart largely from nucleus ambiguus in the cat. J. Auton. Nerv. Syst. 70: 38-50, 1998
40.Hsieh, J.H., J.J. Wu, C.T. Yen and C.Y. Chai The depressor
caudal ventrolateral medulla: its correlation with the pressor dorsomedial and ventrolateral medulla and the depressor paramedian reticular nucleus. J. Auton. Nerv. Syst. 70: 103-14, 1998
41.Huang, Z.S., G.L. Gebber, S. Zhong and S.M. Barman Forced
oscillations in sympathetic nerve discharge. Am. J. Physiol. 263: R564-71, 1992
42.Kenney, M.J., Frequency characteristics of sympathetic nerve
discharge in anesthetized rats. Am. J. Physiol. 267: R830-40, 1994
43.King, G.W. and C.K. Knox Types and locations of respiratory-related neurons in lateral tegmental field of cat medulla oblongata Brain Res. 295: 301-15, 1984
44.Krassioukov, K.V. and L.C. Weaver Connections between the
pontine vericular formation and rostral ventrolateral medulla. Am. J. Physiol. 265: H1368-92, 1993
45.Kuo, T.B.J. and S.H.H. Chan Continuous, on-line, real-time spectral analysis of systemic arterial pressure signals. Am. J. Physiol. 264: H2208-13, 1993
46.Lydic, R. and H.A. Baghdoyan Pedunculopontine stimulation
alters respiration and increases ACh release in the pontine reticular formation. Am. J. Physiol. 264: R544-54, 1993
47.Machado, B.H. and M.J. Brody Effect of Nucleus ambiguus lesion on the deveiopment of neurogenic hypertension. Hypertension 11: I135-38, 1988
48.Machado, B.H. and M.J. Brody Role of the nucleus ambiguus in
the regulation of heart rate and arterial pressure. Hypertension 11: 602-7, 1988
49.Maciewicz, R.J., K. Eagen, C.R.S. Kaneko and S.M. Highstein
Vestibular and medullary brain stem afferents to the abducens nucleus in the cat. Brain Res. 123: 229-40, 1977
50.McKitrick, D.J. and F.R. Calaresu Reciprocal connection between nucleus ambiguus and caudal ventrolateral medulla. Brain Res. 770: 213-20, 1997
51.Mitani, A., K. Ito, A.N. Hallanger, B.H. Wainer, K. Kataoka and R.W. McCarley Cholinergic projections from the laterodorsal and pedunculopontine tegmental nuclei to the pontine gigantocellular tegmental field in the cat. Brain Res. 451: 397-402, 1988
52.Mitani, A., K. Ito, Y. Mitani and R.W. McCarley Descending
projections from the gigantocellular tegmental field in the cat: cells of origin and their brainstem and spinal cord trajectories. J. Comp. Neurol. 268: 546-66, 1988
53.Mitani, A., K. Ito, Y. Mitani and R.W. McCarley Morphological and electrophysiological identificantion of gigantocellular tegmental field neurons with descending projections in the cat: I. Pons. J. Comp. Neurol. 268: 527-45, 1988
54.Mitani, A., K. Ito, Y. Mitani and R.W. McCarley Morphological and electrophysiological identification of gigantocellular tegmental field neurons with descending projections in the cat: II. Bulb. J. Comp. Neurol. 274: 371-86, 1988
55.Miuar, M. and T. Kitamura Postsynaptic potentials recorded from medullary neurones following stimulation of carotid sinus nerve. Brain Res. 162: 261-72, 1979
56.Montano, N., T. Gnecchi-Ruscone, A. Porta, F. Lombardi,
A. Malliani and S.M. Barman Presence of vasomotor and
respiratory rhythms in the discharge of single medullary neurons involved in the regulation of cardiovascular system. J. Auton. Nerv. Syst. 57: 116-22, 1996
57.Nosaka, S., K. Yasunaga and S. Tamai Vagal cardiac preganglionic neurons: distribution, cell types, and reflex discharges. Am. J. Physiol. 243: R92-8, 1982
58.Oka, T., H. Iwakiri and S. Mori Pontine-induced generalized
suppression of postural muscle tone in a reflexively standing acute decerebrate cat. Neurosci. Res. 17: 127-40, 1993
59.Okumura, T., A. Uehara, Y. Taniguchi, Y. Eatanabe, K. Tsuji, S.Kitamori and M. Namiki Kainic acid injection into medullary raphy produces gastric lesions through the vagal system in rats. Am. J. Physiol. 264: G655-58, 1993
60.Pan, C.M., S.D. Wang, C.Y. Chang, H.T. Horng, A. Lin and
C.Y. Chai Afferent and efferent connections of the paramedian reticular nucleus in the brain stem of cats. Chin. J. Physiol. 35: 181-96, 1992
61.Perrone, M.H., Biochemical evidence that L-glutamate is a
neurotransmitter of primary vagal afferent nerve fibers. Brain Res. 230: 283-93, 1981
62.Rimoldi, O., S. Pierini, A. Ferrari, S. Cerutti, M. Pagani and A. Malliani Analysis of short-term oscillations of R-R and arterial pressure in conscious dogs. Am. J. Physiol. 258: H967-76, 1990
63.Rose, J.D., Response properties and anatomical organization of pontine and medullary units responsive to vaginal stimulation in the cat. Brain Res. 97: 79-93, 1975
64.Ruggiero, D.A., S. Tong, M. Anwar, N. Gootman and P.M.
Gootman Hypotension-induced expression of the c-fos gene in the medulla oblongata of piglets. Brain Res. 706: 199-209, 1996
65.Ruiz-Pesini, P., E. Tome, L. Balaguer, J. Romano and M. Yllera The projections to the Medulla of neurons innervating the carotid sinus in the dog. Brain Res. Bull. 37: 41-6, 1995
66.Simonian, N.A., R.L. Getz, J.C. Leveque, C. Konradi and J.T.
Coyle Kainic acid induces apoptosis in neurons. Neuroscience 75: 1047-55, 1996
67.Stocker, S.D., B.C. Steinbacher Jr, C.D. Balaban and B.J. Yates Connections of the caudal ventrolateral medullary reticular formation in the cat brainstem. Exp. Brain Res. 116: 270-82, 1997
68.Stuesse, S.L. and K.S. Powell Cardiac vagal preganglionic fibers in neonatal rats: a comparison with cervical vagal components. Neurosci. Lett. 34: 7-12, 1982
69.Su, C.K., C.T. Yen, J.C. Hwang, C.J. Tseng, J.S. Kuo and C.Y. Chai Differential effects on sympathetic nerve activities elicited by activation of neurons in the pressor areas of dorsal and rostral ventrolateral medulla in cats. J. Auton. Nerv. Syst. 40: 141-54, 1992
70.Talman, W.T., M.H. Perrone and D.J. Reis Evidence for L-
Glutamate as the Neurotransmitter of baroreceptor afferent nerve Fibers. Science 209: 813-15, 1980
71.Tan, J. and G. Holstege Anatomical evidence that the pontine
lateral tegmental field projects to lamina I of the caudal spinal trigeminal nucleus and spinal cord and to the edinger-westphal nucleus in the cat. Neurosci. Lett. 64: 317-22, 1986
72.Ter Horst, G.J., R.W.M. Hautvast, M.J.L. De Jongste and J. KorfNeuroanatomy of cardiac activity-regulating circuitry: a transneuronal retrograde viral labelling study in the rat. Eur. J. Neurosci. 8: 2029- 41, 1996
73.Yates, B.J., C.D. Balaban, A.D. Miller, K. Endo and Y. YamaguchiVestibular inputs to the lateral tegmental field of the cat: potential role in autonomic control. Brain Res. 689: 197-206, 1995
74.Zhong, S., S.M. Barman and G.L. Gebber Effect of brain stem
lesion on 10-Hz and 2-6Hz rhythms in sympathetic nerve discharge. Am. J. Physiol. 262: R1015-24, 1992
75.張益彰. 貓橋腦側蓋降心區與延腦迷走運動神經核間相關性的電
生理研究. 私立中原大學醫學工程學系碩士論文. 中華名國八十六
年.