臺灣博碩士論文加值系統

本試驗的目的是在尋找最適合誘導蝴蝶蘭擬原球體形成，進而探討擬原球體大量增殖，及誘導植株的再生之培養基配方及培養環境條件。蝴蝶蘭Phal. ‘Wedding Promenade’及Dtps. Minho Valentine以花梗節芽為培植體，置於NDM（New Dogashima Medium）培養基誘導擬原球體形成較1/2MS（Murashige and Skoog）、VW（Vacin-Went）及花寶（Hyponex）基本培養基配方為佳。Dtps. Minho Princess以花梗節芽為培植體，置於NDM添加生長調節劑NAA 0.1mg/L與BA 1.0mg/L培養基中，誘導擬原球體形成率為26％，以Dtps. Nobby's Pink Lady ‘T699’× Dtps. Taisuco Firebird ‘DL18’，以花梗節芽為培植體，置於NDM添加生長調節劑NAA 0.1mg/L與BA 1.0mg/L培養基中，對誘導擬原球體形成率為34.6％。Phal. ‘Wedding Promenade’以NDM添加生長調節劑NAA 0.1mg/L與BA 2mg/L培養基中，誘導擬原球體形成率為77％最多，由此可知培養基配方對誘導擬原球體形成亦隨品種差異而有很大的不同。
Dtps. Minho Princess ‘MH903特紅’以短莖為培植體，置於1/2MS和花寶培養基比NDM培養基有利於誘導擬原球體的形成。在Dtps. I-Hsin Actor ‘KH5213’、Phal. Tinny White ‘TT1006’與Dtps. Taisuco Roseherz×Dtps. King Shang's Rose×Dtps. King Sang's Coral以短莖為培植體，置於NDM培養基中以添加蔗糖比麥芽糖與葡萄糖對誘導擬原球體形成好。以NDM培養基中添加醣濃度為0.5％對誘導芽體和擬原球體同時形成的效果最好。
Phal. Taipei Gold ‘T. G.’ × Phal. Black Eagle ‘KH1872’及Dtps. Sinica Ruby ‘KH5498’以擬原球體為培植體，於1/2MS、NDM及花寶培養基配方，除可使擬原球體增殖外，亦可使部分擬原球體再生為芽體。Phal. Taipei Gold ‘T. G.’ × Phal. Black Eagle ‘KH1872’以花寶培養基添加生長調節劑NAA 0.1mg/L對於擬原球體的增殖倍率最高。以花寶培養基添加NAA 0.1mg/L與BA 2mg/L對於平均每個培植體再生所形成的芽體數最多。Dtps. Sinica Rubx ‘KH5498’以花寶培養基不含NAA與BA對於擬原球體的增殖倍率最高。Phal. Taipei Gold ‘T. G.’ × Phal. Black Eagle ‘KH1872’、Dtps. Sinica Ruby ‘KH5498’及Phal. Tinny White ‘TT1006’以擬原球體為培植體，於1/2MS、NDM及花寶培養基配方中培養，以花寶培養基添加NAA 0.1mg/L與BA 4mg/L再生芽體效果最佳。Phal. Taipei Gold ‘T. G.’ × Phal. Black Eagle ‘KH1872’，以花寶培養基不含NAA與BA對於擬原球體的增殖倍率最高，以NAA 0.1mg/L與BA 4mg/L的組合最有利於芽體的再生。而Phal. Taipei Gold ‘T. G.’ ×Phal. Black Eagle ‘KH1872’及Dtps. Sinica Ruby ‘KH5498’培養於花寶培養基不添加NAA與BA不但最有利於擬原球體的增殖亦有利於芽體的再生。

The purpose of this research was to find the best media formula and cultural environment for PLB induction, PLB proliferation and shoot regeneration of Phalaenopsis hybrids. Using lateral buds removed from young flower stalks as explants, NDM（New Dogashima Medium）was the best medium formula for PLB induction in Phal. ‘Wedding promenade’ among the 1/2MS（Murashige and Skoog），VW（Vacin and Went） and Hyponex basal medium formula. The rate of PLB formation for lateral buds cultured on NDM medium containing NAA 0.1 mg/L and BA 1.0mg/L in Dtps. Nobby's Pink Lady ‘T699’ × Dtps. Taisuco Firebird ‘DL18’ and Dtps. Minho Princess was 34.6% and 26% respectively. However, The rate of PLB induction for lateral buds cultured on the NDM medium containing NAA 0.1 mg/L and BA 2.0mg/L in Phal. ‘Wedding promenade’ was 77﹪. Therefore, The effect of basal medium on PLB induction in Phalaenopsis showed lots of variations among the hybrids.
Using short-stems removed from shoots in vitro, 1/2MS and Hyponex media were more available for PLB induction in Dtps. Minho Princess ‘MH903’ than NDM medium. Sucrose in the NDM medium was more productive for PLB induction than maltose and glucose in Dtps. I-Hsin Actor ‘KH5213’、Phal. Tinny White ‘TT1006’ and Phal. Taisuco Roseherz × Dtps. King Shiang’s Rose × Dtps. King Shiang’s Coral. The optimum carbonhydrate concentration in NDM medium for shoot and PLB formation ranged from 0.5%.
PLBs as explants subcultured on 1/2MS, NDM and Hyponex medium formula in Phal. Taipei Gold ‘T. G.’ × Phal. Black Eagle ‘KH1872’ and Dtps. Sinica Ruby ‘KH5498’, not only PLB proliferation occurred, but also shoot regenerated. PLB subcultured on Hyponex medium containing NAA 0.1mg/L showed the highest rate of PLB proliferation in Phal. Taipei Gold ‘T. G.’ × Phal. Black Eagle ‘KH1872’. The highest number of regenerated shoots per explant appeared in the medium containing NAA 0.1mg/L and BA 2mg/L. However, PLB explants subcultured on Hyponex medium free of NAA and BA had the highest rate of PLB proliferation in Dtps. Sinica Ruby ‘KH5498’. It was found that Hyponex medium containing NAA 0.1mg/L and BA 4mg/L was best for shoot regeneration from PLB in Phal. Taipei Gold ‘T. G.’ × Phal. Black Eagle ‘KH1872’. Hyponex medium free of NAA and BA was more available not only PLB proliferation, but also for shoot regeneration in Phal. Taipei Gold ‘T. G.’ × Phal. Black Eagle ‘KH1872’ and Dtps. Sinica Ruby ‘KH5498’.

中文摘要………………….………..……….…..…………………………Ι
前言………………………………………………….…………………………1
前人研究…………………………………………………………….….…...3
材料方法………………………………………………………….……….…16
一、 植物準備與消毒…………………...……..………………...16
二、 培養基配方……………………………..…….……………...17
三、 方法………………………………...…….………...……….17
（一）蝴蝶蘭花梗節潛伏芽之誘導……………….…….……....…….17
（二）蝴蝶蘭擬原球體之誘導…………….….………………………...18
（三）蝴蝶蘭擬原球體之增殖與再生……………………………..…….23
結果…………………….….……………….…….……………………….25
（一）蝴蝶蘭花梗節潛伏芽之誘導…….……….……………….………25
（二）蝴蝶蘭擬原球體之誘導……...…………………….…………….27
（三）蝴蝶蘭擬原球體之增殖與再生…………………….………..……35
討論……………….….….….……………………………………...……84
（一）蝴蝶蘭花梗節潛伏芽之誘導………………………………...……84
（二）蝴蝶蘭擬原球體之誘導…….………..……………….……….…86
（三）蝴蝶蘭擬原球體之增殖與再生………………………………...…92
英文摘要……………………….……………………………..……………94
參考文獻……………………….……………….………….…...……….96

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