臺灣博碩士論文加值系統

所測試的 15 株白腐真菌中, 以 7號分離株對偶氮染料(Orange G)的去色
效果最好, 預先於 1/2 PDB 4 天培養的完整培養液, 在加入100 ppm 染
料後 2 小時, 即有 93% 的去色效果. 7 號分離株的生長適溫是
28-34℃. 測試的三種碳源中 (1%葡萄糖、1%β-纖維素、1%α-纖維素)
, 以1%葡萄糖的利用最好, 產生最大量的菌絲乾重, 對染料的去色效果也
最好. 三種緩衝培養液中(磷酸鉀、醋酸鈉、DMS緩衝培養液),以培養於磷
酸鉀緩衝培養液的菌絲乾重最重, 但三種緩衝培養液於加入染料後 8 小
時測試, 對染料都有 80% 以上的去色效果,顯示菌絲乾重與去色效果無正
相關. 不同培養組成分對 7號分離株培養濾液的去色效果有明顯的差異,
預先培養於 1/2 PDB 4 天的培養濾液,在加入染料後 2 小時,能將 75%
以上的染料去除, 但培養於 LN(低氮)之培養濾液, 完全沒有去色效果.
7 號分離株培養於 HN(高氮)之濾液及漂洗菌絲,其去色效果優於 LN 及
1/2 PDB, 此現象不同於 Phanerochaete chrysosporium的去色作用,因
為 P.chrysosporium 需在養分缺乏的情況下才有去色作用 . 不同緩衝溶
液中以 pH 5、6 的去色效果較好,但所測試的緩衝溶液,對漂洗菌絲的去
色效果有明顯的抑制作用.一般而言,不同培養液會影響酵素的產量, 以培
養於 1/2 PDB培養液可得最高的酵素活性, 但所測試的四種酵素(Lignin
Peroxidase、Manganese dependent Peroxidase、 Laccase、Glyoxal
oxidase)與 Orange G 的去色作用似乎無關.經固定化作用之菌絲小球,其
去色效果與未經固定的菌絲無明顯差異,而不同的固定方法會影響去色效
果,而活性碳的添加與否,並不影響菌絲小球的去色效果.

The capacities of various white rot fungi to decolorize
serveral azo dyes, mainly Orange G, were studied in the pre-
sent study. Among 15 different isolates, isolate 7 showed the
best decolorization ability, which could remove 93% of the
added dye in 2 hours. When isolate 7 was precultured in media
buffered differently(i.e., potassium phosphate、sodium acetate
and DMS buffered medium), the highest dry weight was obtained
in potassium phosphate buffered medium. But, we failed to de-
tect any significant of its decolorization activity. On the
other hand, different medium compositions do show the effect on
the decolorization ability of various white rot fungi. But we
did not observe positive correlation between decolorization
ability and the amount of fungi biomass been produce. When
studying the effect of pH, the best decolorization activity was
observed between 5 and 6. The effect of medium composition on
the production of Laccase, Lignin per-oxidsae, Manganese
dependent peroxidase, and Glyoxal oxidase was also studied.
Regardless, whether it is in the filtrate or on the hyphae,the
highest enzymatic activities were always detected when 1/2 PDB
was used as the cultural medium. Unfortunatelly, I was unable
to detect a correlation between the decolorization ability of
isolate 7 and the production of the above enzymes.