臺灣博碩士論文加值系統

根據調查台灣民眾平均膳食纖維僅達建議攝取量的1/2,而流行病學調查已證實膳食纖維在預防腸癌上扮演重要的角色。腸道黏膜上皮細胞內含有一區域性免疫組織培耶氏斑（peyer’s patches），其是腸道內外來物進入體內首要防禦系統。那麼膳食纖維是否會影響腸道內區域性免疫組織的發育進而影響腸道的免疫功能。我們以三至四週齡Wistar公鼠分成十組，除了對照組飼料中無添加任何纖維質之外，實驗組各餵食不同來源的膳食纖維：纖維素、小麥麩皮、米糠（cellulose、wheat bran 、rice bran）與不同劑量（w/w：5﹪、10﹪、20﹪），十組共餵食八週後犧牲，採心臟動脈血以酵素結合免疫分析法（Enzyme-linked Immunosorbent Assay ；ELISA）分析血清免疫球蛋白IgA、IgG、IgM含量；收集迴腸迴盲瓣端培耶氏斑做石臘包埋切片與冷凍切片。石臘包埋切片利用蘇木紫和伊紅染色，觀察培耶氏斑形態學上組織發育情形；冷凍切片利用免疫染色法觀察培耶氏斑B淋巴球與T淋巴球的分佈與含量。結果由組織切片上可看出經由膳食纖維的餵食，實驗組培耶氏斑濾泡數量較對照組多且實驗組濾泡發育排列緊湊，濾泡與濾泡間區及絨毛發育不具體。冷凍切片免疫染色結果，發現在實驗組在培耶氏斑的圓頂區部位有B細胞被染出，但對照組在培耶氏斑的圓頂區部位沒有B細胞被染出。而酵素結合免疫分析法（ELISA）分析血清免疫球蛋白含量上，發現多組實驗組血清免疫球蛋白IgG與IgM含量較對照組少。結論膳食纖維中纖維素、小麥麩皮和米糠可調節腸道培耶氏斑的發育，在血清免疫球蛋白含量上實驗組含較少IgG與IgM，推測可能與較少抗原刺激造成體內淋巴結免疫球蛋白分泌較少有關，值得再做進一步確認。

According to the study on dietary fiber intake for Taiwanese, only half amount of daily recommended allowance had been taken. Epidemiological investigation also demonstrated that dietary fiber plays an important role in the prevention of colon cancer. Since the gut-associated lymphoid tissue（GALT）-peyer’s patches of the epithelium mucosal cells is the primary defense system of the intestine against the foreign antigen, we wonder if dietary fiber affects the development of GALT and then further affects the intestinal immunity.
Three-to-four-week-old Wistar rats were divided into ten groups. In addition to the control group fed without fiber，the treatment groups were fed with cellulose, wheat bran, and rice bran for 8 weeks with various doses（w/w：5﹪, 10﹪, and 20﹪） respectively .First of all, we drew the blood from heart artery for analyzing serum immunoglobulins IgA、IgG、IgM by method of enzyme-linked immunosorbent assay（ELISA）. Secondly, we collected peyer’s patches（PP.）closed to ileum-cecum value to make paraffin embedding and then observed the development of PP by staining with haematoxylin and eosin . We also performed frozen section of immunohistochemistry to exam the distribution of B lymphocytes and T lymphocytes in PP.
The results showed that there were more follicles in PP for cellulose, rice bran and wheat bran groups than those for control group. We also noticed that no significant inferfollicle areas and villi for the groups fed with different dietary fiber. Frozen section of immunohistochemistry have showed that there were more B cell by staining in the dome area of peyer’s patches for cellulose, rice bran and wheat bran groups than those for control group.
The treatment groups exhibited lesser immunoglobulin G and M compared to control group. It is possible that lower levels of immunoglobulin secreted from lymph nodes were caused by lesser antigen stimulation. It is worthy to be further studied.

表目錄
圖目錄
中文摘要--------------------------------------Ⅰ
英文摘要--------------------------------------Ⅱ
第一章 前言------------------------------------1
第二章 文獻回顧--------------------------------2
第一節 膳食纖維的功能-----------------------2
第二節 一般免疫學---------------------------3
第三節 黏膜相關性淋巴組織-------------------17
第四節 人類培耶氏斑的發育-------------------20
第五節 大鼠培耶氏斑的發育-------------------21
第六節 膳食纖維與免疫功能的關係-------------24
第三章 研究動機與目的--------------------------26
第四章 材料與方法------------------------------27
第五章 結果與討論------------------------------38
第六章 結論------------------------------------55
參考文獻--------------------------------------111

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