臺灣博碩士論文加值系統

Abstract

To determine the relationship between the responses of different mango cultivars exposed to varying chilling temperatures, the chlorophyll fluorescence of excised mango leaves were used aiming to differentiate their behavior under low temperarture stress. Leaves were exposed to seven temperature regimes starting from 25o, serving as the optimum temperature. Followed by chilling temperatures at 12o, 9o, 6o, 3o, 1o and a freezing temperature at -3oC. The flushing position and leaf maturity were quantitative variables used to compare the Fv/Fm ratio in addition to temperature, cultivar and storage period. Results showed that there was a significant decrease in Fv/Fm ratio upon a drastic change to a chilling temperature at 12oC. ‘Mahachanok’ cultivar showed the lowest Fv/Fm ratio while ‘Jin Huang’ had statistically higher followed by ‘Haden’ and ‘Irwin’. Mature leaves were more tolerant than young leaves to low temperature treatment. In general, the flushing position between cultivars indicated two scenarios for the influence of leaf age on individual chilling behavior. Such that in ‘Jin Huang’, the older the leaves the more tolerant it is to the low temperature stress but in ‘Mahachanok’ were found to be more sensitive. These data was able to identify that ‘Jin Huang’, ‘Haden’ and ‘Irwin’ were more chilling tolerant but ‘Mahachanok’ as chilling sensitive.

Table of Contents
Acknowledgement i
Abstract (English) ii
Table of Contents iii
List of Tables vi
List of Figures vii
Chapter 1: Introduction 1
Chapter 2: Review of Literature
2.1. World outlook in Mango Production and Trade 4
2.1.1. Taiwan 5
2.1.2. Philippines 6
2.1.3. Thailand 6
2.2. Mangifera indica L. 7
2.2.1. Taxonomy 7
2.2.2. Origin 8
2.2.3. Types and domestication 8
2.2.4. Growth 9
2.2.5. Inflorescence 10
2.2.6. Fruit setting 10
2.2.7. The mango fruit 11
2.2.8. Environment 12
2.2.9. Breeding 13
2.2.10. Chilling injury in mango 14
2.3. Physiology if chilling stress 15
2.3.1. Chilling injury 16
2.3.2. Physiological responses to chilling injury 17
2.3.3. Cellular changes 18
2.3.4. Effects on Photosynthesis 19
2.3.5. Stomatal functioning 20
2.3.6. Chlorophyll fluorescence 21
Chapter 3: Materials and Methods
3.1. Plant material 23
3.2. Chilling treatment 24
3.3. Chlorophyll fluorescence 25
3.4. Measurement of FRAP, TPC 26
3.5. Electron microscopy 27
3.6. Statistical analysis 28
Chapter 4: Results
4.1. Effect of chilling temperature on Chlorophyll 29
Fluorescence
4.2.. Effect of chilling temperature on FRAP, TPC. 39
4.3. Stomatal movement 39
Chapter 5: Discussions
5.1. Chlorophyll fluorescence 41
5.2. Antioxidant capacity 46
5.3. Stomatal movement 48
Chapter 6: Conclusions 50
References 52
List of Tables

Table 1. Changes in Fv/Fm values in reponse to different levels 29
of chilling temperature.
Table 2. Changes in Fv/Fm values between mango cutivars 30
Table 3. Changes in Fv/Fm values in response to different
flushing position. 30
Table 4. Changes in Fv/Fm values between different storage
period. 31
Table 5. Changes in Fv/Fm values between mango cultivars. 32
Table 6. Changes in Fv/Fm values in response to different
leaf maturity. 32
Table 7. Fv/Fm values between temperature and cultivar. 35
Table 8. Fv/Fm values between temperature and flushing
position. 36
Table 9. Fv/Fm values between cultivar and flushing
position. 37
Table 10. Fv/Fm values between cultivar and storage
period. 37
Table 11. Fv/Fm values between temperature and storage period 38
Table 12. FRAP and TPC values of Irwin and Mahachanok cultivars. 39


List of Figures

Figure 1. Plant material. Flflushing position of the mango leaf in the branch. 24
Figure 2. Leaf preparation for the chilling treatment. 25
Figure 3. Chlorophyll fluorescence instrument. Mini-PAM fluorometer. 26
Figure 4. TM3000-Hitachi tabletop scanning electron microscope. 28
Figure 5. Stomatal characteristic of ‘Mahachanok’ leaves. 40
Figure 6. Stomatal characteristic of ‘Irwin’ leaves. 40

REFERENCES

Journal references
Allan, A.C., M.D. Fricker, J.L. Ward, M.H. Beale and A.J. Trewavas. 1994. Two transduction pathways mediate rapid effects of abscisic acid in Commelina guard cells. Plant Cell 6(9):1319-1328.
Allen, D.J. and D.R. Ort. 2001. Impacts of chilling temperatures on photosynthesis in warm climate plants. Trends Plant Sci. 6(1):36-42.
Allen, D.J., K. Ratner, Y.E. Giller, E.E. Gussakovsky, Y. Shahak and D. Ort. 2000. An overnight chill induces a delayed inhibition of photosynthesis at midday in mango (Mangifera indica L.). J. Exp. Bot. 51(352):1893-1902.
Arthacinta, C. 2000. Mango research in Thailand. Acta Hort 509:33-36.
Assmann, S.M. and K.I. Shimazaki. 1999. The multisensory guard cell. Stomatal responses to blue light and abscisic acid. Plant Physiol. 119:809-815.
Baker, N.R. 2008. Chlorophyll fluorescence: A probe of photosynthesis in vivo. Annu. Rev. Plant Biol. 59:89-113.
Baker, N.R. and E. Rosenqvist. 2004. Applications of chlorophyll fluorescence can improve crop production strategies: an examination of future possibilities. J. Exp. Bot. 15(403):1607-1621.
Bally, I.S.E., P. Lu and P.R. Johnson. 2009. Mango breeding. In: Breeding plantation tree crops: tropical species. S.M. Jain and P.M Priyadarshan (eds.). Springer science + business media, LLC. Pp. 51-82.
Benzie, I.F.F. and J.J. Strain. 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal. Biochem. 239:70-76.
Bompard, J.M. and R.J. Schnell. 1997. Taxonomy and systematics. In: The mango: botany, production and uses. R.E. Litz (ed.) CAB International Press, London. pp. 21-47.
Bozzola, J.J. and L.D. Russel. 1999. Electron microscopy: principles and techniques for biologists. Jones and Bartlett Learning. Pp. 202-221.
Chandler, W.H. 1950. Evergreen orchards. Lea and Febiger. Philadelphia, USA.
Chasan, R. 1995. Internal signals: charting a course through the chloroplast. Plant Cell 7: 1343-1345.
Christiansen, M.N. 1979. Physiological bases for resitance to chilling. HortScience. 14(5): 583-586.
Couey, H.M. 1982. Chilling injury of crops of tropical and subtropical origin. HortScience. 17(2):162-165.
Crane, J.H. 2008. Mangifera indica-Mango. In: The encyclopedia of fruit and nuts. J. Janick and R.E. Paull (eds.) CAB International, Wallingford, Oxon, UK. pp. 15-20.
Davenport, T.L. 1997. Reproductive physiology. In: The mango: botany, production and uses. R.E. Litz (ed.) CAB International Press, London. pp. 69-146.
De Silva, D.L.R., S.J. Honour and T.A. Mansfield. 1996. Estimations of apoplastic concentrations of K+ and Ca2+ in the vicinity of stomatal guard cells. New Phytol. 134(3):463-469.
Eumus, D., R. Fenton and J.M. Wilson. 1983. Stomatal behavior and water relations of chilled Phaseolus vulgaris L. and Pisum sativum L. J. Exp. Bot. 34(141):434-441.
Eun, S.O. and Y.S. Lee. 1997. Actin filaments of guard cells are reorganized in response to light and abscisic acid. Plant Physiol. 115(4):1491-1498.
Evans, E.A. 2008. Recent trends in world and U.S mango production, trade, and consumption. http://edis.ifas.ufl.edu/FE718. Accessed: 2009/09/14.
Fitter, A.H. and R.K.M. Hay. 1987. Environmental physiology of plants, 2nd ed. Academic Press, Inc. San Diego, Ca. pp. 187-197.
Fracheboud, Y., P. Haldimann, J. Leipner and P. Stamp. 1999. Chlorophyll fluorescence as a selection tool for cold tolerance of photosynthesis in maize (Zea mays L.). J. Exp. Bot. 50:1533-1540.
Goguey, T. 1997. Architectural approach of the mechanisms of canopy growth and flowering of mango trees. Acta Hort. 455:124-131.
Goldstein, J., D.E. Newberry, D.C. Joy, P. Echlin, C.E. Lyman and E. Lifshin. 2003. Scanning electron microscopy and X-ray microanalysis. Springer. Pp. 1-7.
Gombos, Z., H. Wada and N. Murata. 1994. The recovery of photosynthesis from low temperature photoinhibition is accelerated by the unsaturation of membrane lipids: a mechanism of chilling tolerance. Proc. Natl. Acad. Sci. 91:8787-8791.
Gonzalez-Aguilar, G.A., J. Fortiz, R. Cruz, R. Baez and C.Y. Wang. 2000. Methyl jasmonate reduces chilling injury and maintains postharvest quality of mango fruit. J. Agric. Food Chem. 48:515-519.
Gray, G.R., L.P. Chauvin, F. Sarhan and N.P.A. Huner. 1997. Cold acclimation and freezing tolerance. Plant Physiol. 114:467-474.
Hugly, S. and C. Somerville. 1992. A role for membrane lipid polyunsaturation in chloroplast biogenesis at low temperature. Plant Physiol. 99:197-202.
Ilan, N., N. Moran and A. Schwartz. 1995. The role of potassium channels in the tempereature control of stomatal aperture. Plant Physiol. 108:1161-1170.
Iyer, C.P.A. and C. Degani. 1997. Classical breeding and genetics. In: The mango: botany, production and uses. R.E. Litz (ed.) CAB International Press, London. pp. 49-68.
Jintanawongse, S. and S. Changtragoon. 2000. Identification of cultivars and certification of hybrids in mango by (Mangifera indica Linn.) by isozyme gene markers. Acta Hort. 509:177-184.
Kane, O., P. Marcellin and P. Mazliak. 1978. Incidence of ripening and chilling injury on the oxidative activities and fatty acid compositions of the mitochondria from mango fruits. Plant Physiol. 61:634-638.
Kaniuga, Z. 2008. Chilling response of plants: importance of galactolipase, free fatty acids and free radicals. Plant Biol. 10:171-184.
Karihaloo, J.L., Y.K. Dwivedi, Sunil-Archak and A.B. Gaikwad. 2003. Analysis of genetic diversity of Indian mango cultivars using RAPD markers. J. Hort. Sci. Biotech. 78(3):285-289.
Knight, R.J. 1997. Important mango cultivars and their descriptors. In: The mango: botany, production and uses. R.E. Litz (ed.) CAB International Press, London. pp. 545-565.
Kostermans, A.J.G.H. and J.M. Bompard. 1993. The mangoes. Botany, nomenclature, horticulture, and utilization. Academic Press, London.
Kratsch, H.A. and R.R. Wise. 2000. The ultrastructure of chilling stress. Plant Cell Environ. 23:337-350.
Krishna, H. and S.K. Singh. 2007. Biotechnological advances in mango (Mangifera indica L.) and their future implication on crop improvement-a review. Biotech. Advances 25:223-243.
Kuk, Y.I. and J.S. Shin. 2007. Mechanisms of low-temperature tolerance in cucumber leaves of various ages. J Amer. Soc. Hort. Sci. 132(3):294-301.
Kuk, Y.I., J.H. Lee, H.Y. Kim, S.J. Chung, G.C. Chung, J.O. Guh, H.J. Lee and N.R. Burgos. 2003. Relationships of cold acclimation and antioxidative enzymes with chilling tolerancein cucumber (Cucumis sativus L.). J Amer. Soc. Hort. Sci. 128(5):661-665.
Kumar, N.V.H., P. Narayanaswamy, D.T. Prasad, G.K. Mukunda and S.N. Sondhur. 2001. Estimation of genetic diversity of commercial mango ((Mangifera indica L.) cultivars using RAPD markers. J. Hort. Sci. Biotech. 76(5):529-533.
Kyle, D.J., I. Ohad and C.J. Arntzen. 1984. Membrane protein damage and repair: selective lossof a quinine-protein function in chloroplast membranes. Proc. Natl. Acad. Sci. 81:4070-4074.
Lafuente, M.T., A. Belver, M.G. Guye and M.E. Saltveit. 1991. Effect of temperature conditioning on chilling injury of cucumber cotyledons. Plant Physiol. 95:443-449.
Lurie, S. and J.D. Klein. 1991. acquisition of low temperature tolerance in tomatoes by exposure to high temperature stress. J Amer. Soc. Hort. Sci. 116(6):1007-1012.
Lyons, J.M., T.A. Wheaton and H.K. Pratt.1964. Relationship between the physical nature of mitochondrial membranes and chilling sensitivity in plants. Plant Physiol. 39:262-268.
Malundo, T.M.M. R.L. Shewfelt, G.O. Ware and E.A. Baldwin. 2001. Sugars and acids influence flavor properties of mango (Mangifera indica). J Amer. Soc. Hort. Sci. 126(1):115-121.
Maxwell, K, and G.N. Johnson. 2000. Chlorophyll fluorescence - a practical guide. J. Exp Bot. 51:659-668.
McAinsh, M.R., A.A.R. Webb, J.E. Taylor and A.M. Hetherington. 1995. Stimulus-induced oscillations in guard cell cytosolic free calcium. Plant Cell 7(2): 1207-1219.
Melcarek, P.K. and G.N. Brown. 1977. The effects of chilling stress on the chlorophyll fluorescence of leaves. Plant Cell Physiol. 18:1099-1107.
Morris, L.L. 1982. Chilling injury of horticultural crops: an overview. HortSci. 17(2):161-162.
Mukherjee, S.K. 1997. Introduction: botany and importance. In: The mango: botany, production and uses. R.E. Litz (ed.) CAB International Press, London. pp. 1-19.
Muller, P., X.P. Li and K.K. Niyogi. 2001. Non-photochemical quenching. A response to excess light energy. Plant Physiol. 125:1558-1566.
Murata, N. and D.A. Los. 1997. Membrane fluidity and temperature perception. Plant Physiol. 115(3):875-879.
Murata, N. 1983. molecular species composition of phosphatidylglycerols from chilling-sensitive and chilling-resistant plants. Plant Cell Physiol. 24(1):81-86.
Namuco, L. and C.J. Andam. 2006. Mango production manual . Philippine council for agriculture, forestry and natural resources research and development, Los Banos, Laguna. PCARRD, 167-C. pp. 43-96.
Noratto, G.D., M.C. Bertoldi, K. Krenck, S.T. Talcott, P.C. Stringheta and S.U.M. Talcott. 2010. Anticarcinogenic effects of polyphenolics from mango (Mangifera indica) varieties. J. Agric. Food Chem. 58:4104-4112.
Nunes, M.C.N., J.P. Emond, J.F. Brecht, S. Dea and E. Proulx. 2007. Quality curves for mango fruit (cv. Tommy Atkins and Palmer) stored at chilling and nonchilling temperatures. J. Food. Qual. 30:104-120.
Nurliyana, R., S. Zahir I., K. Mustapha Suleiman, M.R. Aisyah and K. Kamarul Rahim. 2010. Antioxidant study of pulps and peels of dragon fruits: a comparative study. Intl. Food Res. J. 17:367-375.
Ort, D.R. and B. Martin. 1983. Chilling-induced inhibition of photosynthesis in tomato. In: effects of stress on photosynthesis. R. Marcelle, H. Clijsters and M. Van Poucke (eds.). Martinus Nijhoff/Dr. W. Junk Publishers. The Hague, Netherlands. pp. 227-235.
Phumicai, C. and J. Chunwongse. 1999. DNA fingerprint identification of mango cultivars ‘Mahachanok’, ‘Nang Klang Wan’ and ‘Sunset’. Katsetsart University. Bangkok, Thailand.
Potvin, C. 1985. Effect of leaf detachment on chlorophyll fluorescence during chilling experiments. Plant Physiol. 78:883-886.
Samson, J.A. 1986. Tropical fruits, 2nd ed. Longman Inc. New York.
Sauco, V.G. 2004. Mango production and world market: current situation and future prospects. Acta Hort. 645:107-116.
Sharom, M., C. Willemort and J.E. Thompson. 1994. Chilling injury induces lipid phase changes in membranes of tomato fruit. Plant Physiol 105:305-308.
Shu, Z.H., C.R. Yen, L.S. Ke, D.N. Wang, T.S. Lin and C.C. Shiesh. 2000. Mango production in Taiwan. Acta Hort. 509:87-94.
Smillie, R.M. and S.E. Hetherington. 1983. Stress tolerance and stress-induced injury in crop plants measured by chlorophyll fluorescence In vivo. Plant Physiol. 72:1043-1050.
Sonoike, K. 1998. Various aspects of inhibition of photosynthesis under light/chilling stress: “photoinhibition at chilling temperatures” versus “chilling damage in the light”. J. Plant Res. 111:121-129.
Taiz, L and E. Zeiger. 2006. Plant physiology, 4th ed. Sinauer associates, Inc. Massachusetts, USA.
Tijskens, L.M.M., E.C. Otma and O. van Kooten. 1994. Photosystem II quantum yield as a measure of radical scavengers in chilling injury in cucumber fruits and bell peppers. Planta. 194:478-486.
Tokuhisa, J., J.R. Wu, M. Miquel, Z.G. Xin and J. Browse. 1998. investigating the role of lipid metabolism in chilling and freezing tolerance. In: Plant cell hardiness: molecular biology, biochemistry and physiology. P. Li and T.H.H. Chen (eds.) Plenum press, New York.
Urban, L. and M. Jannoyer. 2004. Functioning and role of stomata in mango leaves. Acta Hort. 645:441-446.
Urban, L., M. Jannoyer, X. Le Roux, H. Sinoquet and S. Jaffuel. 2004. A model of leaf photosynthesis for mango cv. Lirfa. Acta Hort. 645:411-416.
Vallejos, C.E. 1991. Low night temperatures have a differential effect in the diurnal cycling of gene expression in cold-sensitive and tolerant tomatoes. Plant Cell Environ. 14:105-112.
Van Hasselt, P.R. J. Woltjes and F. De Jong. 1983. The effect of temperature on chlorophyll fluorescence induction of cucumber lines differing in growth capacity at suboptimal conditions. In: effects of stress on photosynthesis. R. Marcelle, H. Clijsters and M. Van Poucke (eds.). Martinus Nijhoff/Dr. W. Junk Publishers. The Hague, Netherlands. pp. 257-265.
Venema, J.H., M.Eekhof and P.R. Van Hasselt. 2000. Analysis of low-temperature tolerance of a tomato (Lycopersicon esculentum) cybrid with chloroplasts from a more chilling tolerant L. hirsutum accession. Ann. Bot. 85:799-807.
Walker, M.A., B.D. McKersie and K.P. Pauls. 1991. Effects of chilling on the biochemical and functional properties of thylakoid membranes. Plant Physiol. 97:663-669.
Wang, C.Y. 1994. Chilling injury of tropical horticultural commodities. HortSci. 29(9):986-988.
Wang, C.Y. 1982. Physiological and biochemical responses of plants to chilling stress. HortScience. 17(2):173-186.
Ward, J.M. and J.I. Schroeder. 1994. Calcium-activated K+ channels and calcium-induced calcium release by slow vacuolar ion channels in guard cell vacuolesimplicated in the control of stomatal closure. Plant Cell 6(5):669-683.
Weng, J.H., L.H. Jhaung, J.Y. Jiang, G.M. Lai and T.S. Liao. 2006. Down-regulation of photosystem 2 efficiency and spectral reflectance in mango leaves under very low irradiance and varied chilling treatments. Photosynthetica 44(2):248-254.
Wilkinson, S., A.L. Clephan and W.J. Davies. 2001. Rapid low temperature-induced stomatal closure occurs in cold-tolerant Commelina communis leaves but not in cold-sensitive tobacco leaves, via a mechanism that involves apoplastic calcium but not abscisic acid. Plant Physiol. 126:1566-1578.
Xiong, L.M., K.S. Schumaker and J.K. Zhu. 2002. Cell signaling during cold, drought and salt stress. Plant Cell S165-S183.
Zhao, Z.L., J.K. Cao, W.B. Jiang, Y.H. Gu and Y.M. Zhao. 2009. Maturity-related chilling tolerance in mango fruit and the antioxidant capacity involved. J. Sci. Food Agric. 89:304-309.
Zhou, Y.H., J.Q. Yu, W.H. Mao, L.F. Huang, X.S. Song and S. Nogues. 2006. Genotypic variation of rubisco expression, photosynthetic electron flow and antioxidant metabolism in the chloroplasts of chill-exposed cucumber plants. Plant Cell Physiol.47(2):192-199.
Electronic references
BAS. 2008. Selected Philippine exports: percentage share in worl market, 2004-2005. In: selected statistics on agriculture, 2008. http://countrystat.bas.gov.ph/documents/selected_stat2008.pdf. Accessed: 2009/08/12.
BAS. 2005. Mango: trends in production, prices and exports, 2000-2004. http://bas.gov.ph/downloads_view.php?id=122. Accessed: 2009/08/18.
COA. 2008a. Mango: fresh and processed exports, volume and value, 2008. http://agrapp.coa.gov.tw/TS2/TS20108.htm. Accessed: 2009/09/08.
COA. 2008b. Mangos-agriculture statistics yearbook, 2008. http://www.coa.gov.tw/view.php?catid=19675. Accessed: 2009/09/08.
DOA-Information center. 2009. Mango. Thai Fruit J. http://www.thethaifruit.com/en/journal.php?sxEntry10=13. Accessed: 2010/03/24.
FAOSTAT. 2007. FAO Statistics, food and agriculture organization of the United Nations, Rome, Italy. http://faostat.fao.org/. Accessed: 2009/09/14.