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Morphological traits,isozymes and randomly amplified polymor-
phic DNA (RAPD) markers were used to study the identification
of Capsicum species.The purposes of this study were to
establish molecular markers for classification of the Capsicum
spp., to establish the genetic linkage relationships and a
quick, technique for the identification of seed purity of
Capsicum spp. The results of isozyme analysis indicated that
there were significantly different in the zymograms of EST,
GOT, PGI, PGM, PRX, SkDH and SOD between C. annuum L. and C.
chinense Jacquin. Distinctly polymorphic patterns were
exhibited among the C. annuum L., but only three lines can be
distinguished. Besides, the isozyme patterns might vary with
different extracting buffers and sampling positions. For
example, the expressions of PRX, SOD and PGI were clearer in
lower leaf, GOT, PGM and SkDH in middle leaf and EST in upper
leaf. A total of 10 primers was selected from 300 random
primers that showed the polymorphic patterns in Capsicum spp..
Among , the RAPD patterns of six primers,i.e."UBC 313","UBC
327" , "UBC 346", "UBC 457","UBC 483" and "UBC 484", could
clearly eight tested Capsicum lines and might be used as an
effective for the identification of F1 hybrids and cultivated
varieties of Capsicum spp.. The results of linkage cluster
analysis revealed that the genetic background of 12 cultivars
was very similar, and might originate from P852. Among the
five inbred lines of sweet pepper, P1717 had a closer relation
with P852, next by P1657 and P1740, but P 859 was farthest.The
three hot pepper lines belonged to different population, when
compared with these five inbred lines of sweet pepper. The
results suggested that the RAPD markers is preciser and more
sensitive than morphological and isozyme identifications and
can be used as an effective tool to distinguish plant
germplasms.
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