臺灣博碩士論文加值系統

本研究擬針對敘利亞倉鼠之餵食及實驗方法，包括探討餵食及禁食對血脂之影響，以及觀察餵食木寡醣對倉鼠在飲食及排便方面之變化。實驗結果顯示，倉鼠每小時攝食量範圍在0 g-1.24 g，倉鼠在預期的睡眠期仍有攝食之行為，活動期攝食量較睡眠期高，活動期攝食量與睡眠期攝食量相比約5:3。
進食時間與禁食與否對血液生化值之影響，在總膽固醇方面，餵食8小時+禁食12小時組顯著(p < 0.05)高於餵食16小時+禁食12小時組，餵食8小時組與餵食8小時+禁食12小時組及餵食16小時組與餵食16小時+禁食12小時組有顯著(p < 0.05)差異。在三酸甘油酯方面，進食時間對三酸甘油酯無顯著差異，禁食12小時會使其濃度下降約47 mg/dL。
餵食倉鼠木寡醣並觀察其每隔四小時，在攝食量、飲水量及糞便取樣時間對部分糞便檢測之可能影響，觀察到攝食量與飲水量有相似之趨勢，且發現每時段皆有高低變化，並非定值，故收集糞便樣品進行分析時應儘量固定時間，給予飼料時間亦應固定，使實驗動物適應實驗模式，以降低人為造成之誤差。

The objective of this study was to investigate the feeding and fasting time for Syrian hamsters on blood lipids. Changes in food and water intake as well as fecal excretion were observed. Our results showed that the average food intake was 0 g-1.24 g per hour. It was found that the feeding behavior was continued even in the sleeping period. The amount of food intake in the active period was higher than that in the sleeping period. The ratio of the food intakes in the active and sleep periods was found to be 5:3.
Regarding the influences of feeding time and fasting time on blood lipids, the animal group (fed for 16 hours followed by fasting for 12 hours) had a lower (p < 0.05) level of serum total cholesterol compared with the group (fed for 8 hours followed by fasting for 12 hours). After feeding for 8 hours, fasting for 12 hours resulted in a significant (p < 0.05) decrease of serum total cholesterol. Animal group (fed for 16 hours followed by fasting for 12 hours) was found to have a decreased (p < 0.05) level of serum total cholesterol than those only fed for 16 hours. Regarding the level of serum triglycerides, variations in the feeding time did not result in any apparent changes among different animal groups. However, the treatment of fasting for 12 hours would result in a decrease (-47 mg/dL) of serum triglyceride.
After feeding hamsters with xylooligosaccharides, the effects of food intake, water intake, and fecal sampling time on the fecal samples were determined every 4 hour. It was observed that the food and water intakes during the whole experimental period were similar in pattern but the values in each 4 hour interval were varied. Therefore, it was concluded that the variations in feeding time and specimen collection time should be kept as low as possible. This allowed the experimental animals to adapt to environment changes, hence to increaseconsistency and reduce error in the analyses of blood, feces, or other biological specimens.

中文摘要 i
英文摘要 ii
目錄 iv
表次 vi
圖次 vii

1. 前言 1
1.1 倉鼠 1
1.2 倉鼠之常見研究2
1.3 一般倉鼠實驗操作5
1.4 禁食模式9
2. 研究目的11
3. 材料與方法12
3.1 實驗架構12
3.1.1 第一部分實驗12
3.1.2 第二部分實驗12
3.1.3 第三部分實驗12
3.2 實驗動物飼養 16
3.3 實驗動物之血液取樣16
3.3.1 血清中三酸甘油酯濃度之測定16
3.3.2 血清中總膽固醇濃度之測定17
3.4 實驗動物糞便取樣17
3.4.1 糞便水分含量的測定18
3.4.2 糞便酸度測定18
3.5 統計分析19
4. 結果與討論20
4.1 攝食量20
4.2 血液生化值23
4.2.1 總膽固醇25
4.2.2 三酸甘油酯27
4.3 第三部分每隔四小時倉鼠生理變化29
4.3.1 攝食量變化29
4.3.2 飲水量變化31
4.3.3 糞便顆數、重量、水分含量及乳酸含量之變化35
5. 總結論45
6. 參考文獻46

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