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研究生:廖偉全
研究生(外文):Liao, Wei-Chuan
論文名稱:中藥在高血脂兔子的DNA氧化傷害與修補角色
論文名稱(外文):The role of Chinese herb essences on oxidative DNA damage and repair in the rabbit model of hypercholesteremia
指導教授:張文正、江素瑛
指導教授(外文):Chang, Wen-Cheng、Chiang, Su-Yin
學位類別:碩士
校院名稱:中國醫藥學院
系所名稱:醫學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:2003
畢業學年度:91
語文別:中文
中文關鍵詞:氧化DNA傷害、高效液相層析儀、8-OHdG、高血脂、DNA修補酵素
外文關鍵詞:oxidative DNA damage、HPLC-ECD、8-OHdG、hypercholesteremia、DNA reapir enzyme
相關次數:
  • 被引用被引用:1
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  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:1
粥狀動脈硬化的形成是一種慢性的心臟血管疾病,而粥狀動脈硬化主要是因為血液中膽固醇及低密度脂蛋白在血漿中的含量過高,受到自由基氧化,形成氧化態的脂質,進而形成內皮增生及粥狀動脈斑塊之現象。本實驗利用含有0.5%膽固醇飼料,分別給予山藥、CW-3、大麥苗、川芎、厚朴、丹蔘、紅花、石斛與Ellagic acid,餵食體重約2-3公斤的紐西蘭白兔12週。因為8-OHdG是一個主要的DNA氧化傷害指標,所以我們以HPLC-ECD/UV來偵測8-OHdG的含量,就能知道DNA氧化傷害的情形。結果發現膽固醇組的肝臟較其他組有較高的8-OHdG含量,甚至比對照組高達17倍之多;主動脈的8-OHdG含量則高於對照組接近約4倍,與其他組也有明顯的差異。因此中草藥可以減少DNA氧化傷害的情形,也提供了減弱粥狀動脈硬化形成的治療或預防新配方。同時我們也想了解它的DNA修補機制,如果能充分了解基因與蛋白質的表現情形,或許能調控或啟動修補系統,使人類更能增加自我對抗DNA氧化傷害的威脅。
Atherosclerosis is a chronic inflammatory disease. New Zealand White (NZW) rabbits were assigned randomly into six and eleven treated groups. The normal group was fed regular rabbit chow and the cholesterol group was fed a chow containing 0.5% cholesterol. 8-hydroxy-deoxyguanosine (8-OH-dG) is a main index of oxidatively damage DNA. We extracted DNA in the liver tissues and blood vessel of NZW rabbits. Sample analysis was performed using high-performance liquid chromatography /electrochemical detector (HPLC-ECD). These data revealed that 8-OHdG increased significantly in liver and blood vessels of high cholesterol-fed rabbits than other treated groups. The levels of 8-OHdG between the cholesterol group and the normal group was highest for 17 folds. Treatment with various Chinese herb essences significantly decrease the levels of 8-OHdG, as compared to the cholesterol group. These results suggest that the antioxidant and antiatherogenic effects of Chinese herb essences can be useful in the prevention of atherogenesis. We also investigated the expression of proteins in DNA repair system. This may provide a possible tool to modulate the mechanism against DNA damage in human.
目錄
圖目錄--------------------------------------------------------1
英文圖目錄----------------------------------------------------3
中文摘要------------------------------------------------------5
英文摘要------------------------------------------------------6
第一章 前言---------------------------------------------------7
第一節 高血脂症 (hypercholesteremia)及粥狀動脈硬化
(atherosclerosis)之關係
第二節 自由基 (Free radical)之簡介
第三節 8-hydroxy-2’-deoxyguanosine (8-OHdG)之簡介
第四節 與8-OHdG相關的因子
第五節 與8-OHdG相關的疾病
第六節 高效液相層析技術 (HPLC)之簡介
第七節 氧化DNA修補系統 (Oxidative DNA repair system)
第八節 西藥之藥理作用與臨床應用
第九節 中草藥之藥理作用與臨床應用
第二章 實驗目的----------------------------------------------36
第三章 實驗材料與方法----------------------------------------37
第一節 誘導高血脂症兔子之動物模式Ⅰ
第二節 誘導高血脂症兔子之動物模式Ⅱ
第三節 DNA 萃取
第四節 DNA定量與酵素切割
第五節 利用HPLC-ECD/UV定量8-OHdG
第六節 兔子肝臟蛋白質之萃取
第七節 兔子肝臟蛋白質之定量
第八節 蛋白質電泳 (SDS-PAGE)
第九節 西方點墨法 (Western Blot)
第十節 統計分析法
第四章 結果--------------------------------------------------53
第一節 8-OHdG和dG的標準曲線圖
第二節 DNA氧化傷害的比較
第三節 蛋白質的標準曲線圖
第四節 DNA修補酵素的表現
第五章 討論--------------------------------------------------74
第一節 西藥對粥狀動脈硬化之DNA氧化傷害情形
第二節 中藥對粥狀動脈硬化之DNA氧化傷害情形
第三節 DNA修補酵素的表現
第四節 DNA萃取方法之探討
第五節 HPLC分析方法之探討
第六節 使用HPLC需注意事項
第六章 參考文獻----------------------------------------------86
第七章 附錄-------------------------------------------------100
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