臺灣博碩士論文加值系統

紫苞舌蘭(S. plicata Blume)原球體培植體置於0-30 mg/l 2,4-D與 0-5 mg/l TDZ的1/2 MS 培養基，在未含生長調節劑的培養基中，芽體誘導率較佳；而含有2,4-D的培養基中，較易有癒傷組織產生。培植體表面直接產生多數芽，而不經癒傷組織，此有助紫苞舌蘭大量繁殖。
紫苞舌蘭原球體癒傷組織，置於不含生長調節劑之培養基，照光培養，先形成類原球體，後能繼續發育成植株。將繼代於含生長調節劑中八種癒傷組織，以在2 mg/l Picloram及1 mg/l TDZ繼代之癒傷組織有較高增殖率(8.08倍)。癒傷組織增殖，顯示蔗糖為必要成分；果糖或葡萄糖亦可取代蔗糖；活性碳則造成癒傷組織形態發生。
紫苞舌蘭之根尖，置於單獨添加2,4-D或是2,4-D與TDZ的1/2 MS培養基，60天後形成癒傷組織。將繼代於3 mg/l 2,4-D培養基之癒傷組織，置於含有TDZ與NAA之培養基中，行光照培養，無再生植株產生，可能受原繼代培養基之影響。
紫苞舌蘭小植株之完整葉片置於不含生長調節劑或只含TDZ的1/2 MS培養基中，70天後，葉基部形成小苗或芽體；在含3 mg/l 2,4-D的培養基中，獲得高癒傷組織誘導率(66.8﹪)。由葉基形成之芽體，持續培養，可長成小苗。
紫苞舌蘭之短縮莖在不含生長調節劑的1/2 MS培養基中，全暗培養，可直接產生叢生芽體，經照光後，可得再生植株。短縮莖培養於光照下易褐化，但在不含生長調節劑或只含TDZ下，有小苗、類原球體及叢生芽形成；而加入2,4-D，易產生癒傷組織。

Protocorm explants of S. plicata Blume were cultured on 1/2-MS medium containing 0-30 mg/l 2,4-D and 0-5 mg/l TDZ. The plain basal medium (devoid any hormones additive) were found as the best medium for shoot bud formation and the media supplemented with 2,4-D were effective to induce callus. Shoot buds derived from the epidermal cells of the protocorm explants without intermediate callus formation. The method is useful for mass propagation of S. plicata Blume.
The protocorm-derived callus of S. plicata Blume formed protocorm-like bodies, and regenerated plant resulted by cultured on the 1/2 MS medium without growth regulators under illumination. Eight kind of callus were sub cultured, the best proliferation (8.08) was found the callus subcultured on the medium containing 2 mg/l picloram and1 mg/l TDZ. In callus proliferation, sucrose was component, fructose and glucose instead and charcoal effect the callus morphogenesis.
Root tips of S. plicata Blume formed callus on the 1/2-MS medium containing 2,4-D or 2,4-D and TDZ in 60 days. The callus maintaining on a medium containing 3 mg/l 2,4-D were failed to produce plant on the medium containing TDZ and NAA in light.
The intact leaf explants of Spathoglottis plicata Blume formed shoots or buds from leaf base on the 1/2 MS medium without growth regulators or supplemented with TDZ alone within 70 days. And the high percentage (66.8﹪)of callus inducing were found on a medium supplemented with 3 mg/l 2,4-D. These buds formed from leaf base developed into normal plantlets.
Shoot buds directly derived from stem explants of S. plicata Blume were cultured on 1/2 MS medium alone in darkness. And shoot buds develop normal plants by transplant under light. Stem explants were brown easily when cultured under light. On the medium without growth regulators or plus TDZ alone, stems form shoots, protocom-like body and buds. On the medium plus 2,4-D, stem was easy to callusing.

1.緒論……………………………………………………………………1
1.1.紫苞舌蘭(Spathoglottis plicata Blume)的植物學特性……1
1.1.1.蘭科植物的特徵…………………………………………………1
1.1.2.苞舌蘭屬(Spathoglottis Blume) ……………………………1
1.1.3.紫苞舌蘭(S. plicata Blume)的生長習性……………………2
1.2.蘭科植物的組織培養………………………………………………2
1.3.紫苞舌蘭之組織培養………………………………………………3
1.4.參考文獻……………………………………………………………3
2.紫苞舌蘭原球體培植體之癒傷組織誘導與芽體發生………………7
2.1.前言…………………………………………………………………7
2.2.材料與方法…………………………………………………………7
2.3.結果…………………………………………………………………8
2.4.討論…………………………………………………………………9
2.5.中文摘要……………………………………………………………9
2.6.英文摘要……………………………………………………………9
2.7.參考文獻……………………………………………………………9
3.紫苞舌蘭原球體癒傷組織繼代培養及植株再生……………………16
3.1.前言…………………………………………………………………16
3.2.材料與方法…………………………………………………………16
3.3.結果…………………………………………………………………17
3.4.討論…………………………………………………………………18
3.5.中文摘要……………………………………………………………18
3.6.英文摘要……………………………………………………………19
3.7.參考文獻……………………………………………………………19
4.紫苞舌蘭根培植體癒傷組織誘導及癒傷組織形態發生……………34
4.1.前言…………………………………………………………………34
4.2.材料與方法…………………………………………………………34
4.3.結果…………………………………………………………………34
4.4.討論…………………………………………………………………35
4.5.中文摘要……………………………………………………………35
4.6.英文摘要……………………………………………………………35
4.7.參考文獻……………………………………………………………36
5.紫苞舌蘭葉培植體之形態發生………………………………………43
5.1.前言…………………………………………………………………43
5.2.材料與方法…………………………………………………………43
5.3.結果…………………………………………………………………43
5.4.討論…………………………………………………………………44
5.5.中文摘要……………………………………………………………44
5.6.英文摘要……………………………………………………………44
5.7.參考文獻……………………………………………………………44
6.紫苞舌蘭短縮莖培植體之培養………………………………………49
6.1.前言…………………………………………………………………49
6.2.材料與方法…………………………………………………………49
6.3.結果…………………………………………………………………50
6.4.討論…………………………………………………………………50
6.5.中文摘要……………………………………………………………50
6.6.英文摘要……………………………………………………………50
6.7.參考文獻……………………………………………………………51
7.結論……………………………………………………………………57
8.附錄……………………………………………………………………59

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