臺灣博碩士論文加值系統

厭氧氨氧化程序（ANaerobic AMMonium OXidation process，簡稱ANAMMOX）係以氨氮為電子供給者，亞硝酸鹽氮為電子接受者，將氨氮與亞硝酸鹽氮轉變為氮氣及硝酸鹽氮。此程序可有效縮短傳統生物除氮之流程，降低操作之成本及複雜性，具有極高之研發價值與應用潛力，現已成為國際研究之主流。然而，國內至今相關研究甚少，亦無類似之廢水處理實廠。本研究花費六個月的時間馴養出本土性之厭氧氨氧化菌，並藉由重複之批次試驗探討不同含氮化合物（氨氮、亞硝酸鹽氮、硝酸鹽氮等）、溶氧、磷酸鹽、無機碳源等對於厭氧氨氧化菌脫氮速率之影響。實驗結果顯示，當亞硝酸鹽氮濃度超過75 mg/L以上會對厭氧氨氧化菌產生嚴重之抑制作用，當亞硝酸鹽濃度達100 mg/L時反應幾乎停止。另厭氧氨氧化菌開始反應之時間會隨著氨氮濃度的提高而延後。此外，硝酸鹽氮無法代替亞硝酸鹽氮為最終電子接受者進行厭氧氨氧化反應，且其對厭氧氨氧化之速率亦無明顯之影響。磷酸鹽濃度太高（達3300 mg/L）會對厭氧氨氧化反應造成抑制，無機碳源之濃度低於250 mg/L則無法進行厭氧氨氧化反應。
最後，研究之結果亦顯示，只有氨氮及少量溶氧存在之條件下，氨氮會被部分硝化成亞硝酸鹽氮，當溶氧被用完後，剩餘之氨氮及部分硝化產生之亞硝酸鹽氮會產生厭氧氨氧化作用，達成生物除氮之效果

Anaerobic ammonium oxidation (ANAMMOX) process is an innovative method to remove ammonium from wastewater. ANAMMOX bacteria use ammonium as the electron donor and nitrite as the electron acceptor to convert ammonium to dinitrogen gas under anaerobic condition. Therefore shortcut the biological ammonium removal process and reduce the operation cost of the bioreactor. In this study, ANAMMOX bacteria were cultivated in six months. The factors affecting the nitrogen removal rate of the ANAMMOX bacteria were evaluated via a series of batch experiments.
The experimental results indicated that ANAMMOX reaction delayed under high ammonium concentration. The concentration of nitrite over 75 mg/L inhibited the ANAMMOX reaction seriously. Nitrate couldn’t replace nitrite as the electron acceptor for ANAMMOX reaction in this research. In addition, high phosphate-P concentration (more than 3300 mg/L) or low inorganic carbon source (less than 250 mg/L) inhibited the ANAMMOX reaction also. In addition, the combination of partial nitrification and ANAMMOX reaction was observed under the batch experiments containing ammonium and dissolved oxygen.

目錄
博碩士論文電子檔案上網授權書 II
論文口試委員會審定書 III
摘要 IV
Abstract VI
誌謝 VIII
目錄 IX
表目錄 XI
圖目錄 XII
縮寫、符號說明 XV
第一章 前言 1
1-1 研究緣起 1
1-2 研究目的 3
第二章 文獻回顧 4
2-1 生物除氮之基本原理 4
2-1-1 硝化作用 4
2-1-2 脫硝作用 6
2-2 生物除氮之最新發展 8
2-2-1 部分硝化程序 8
2-2-2 SHARON process 8
2-3 厭氧氨氧化程序（ANAMMOX process） 9
2-3-1 厭氧氨氧化微生物及生化特性 11
2-3-2 厭氧氨氧化菌之馴養 13
2-3-3 SHARON-ANAMMOX process 14
2-3-4 CANON process 15
第三章 研究設備與方法 17
3-1 研究架構 17
3-2 厭氧氨氧化菌之馴養 18
3-3 不同影響因子之批次試驗 20
3-4 研究設備與儀器 21
3-5 分析方法 21
3-6 除氮速率之計算 23
第四章 結果與討論 24
4-1 厭氧氨氧化菌之馴養結果 24
4-1-1 污泥外觀及特性 24
4-1-2 ANAMMOX反應之確認 25
4-2 不同含氮化合物對厭氧氨氧化程序之影響 26
4-2-1 亞硝酸鹽氮濃度對厭氧氨氧化程序之影響 26
4-2-2 氨氮濃度對厭氧氨氧化程序之影響 32
4-2-3 硝酸鹽氮濃度對厭氧氨氧化程序之影響 38
4-2-4 相同亞硝酸鹽氮及氨氮濃度下硝酸鹽氮濃度對厭氧氨氧化程序之影響……… 44
4-3 不同溶氧濃度對厭氧氨氧化程序之影響 49
4-3-1 溶氧濃度對厭氧氨氧化程序之影響（無攪拌） 49
4-3-2 溶氧濃度對厭氧氨氧化程序之影響（攪拌） 53
4-3-3 相同溶氧濃度下氨氮濃度對厭氧氨氧化程序之影響（無攪拌） 58
4-3-4 相同亞硝酸鹽氮及氨氮濃度下溶氧濃度對厭氧氨氧化程序之影響（攪拌）……. 63
4-4 其它因素對厭氧氨氧化程序之影響 68
4-4-1 磷酸鹽濃度對厭氧氨氧化程序之影響 68
4-4-2 無機碳濃度對厭氧氨氧化程序之影響 73
4-4-3 無機碳濃度對厭氧氨氧化程序之影響 78
第五章 結論與建議 84
5-1 結論 84
5-2 建議 86
第六章 參考文獻 87
第七章 簡歷 93

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