臺灣博碩士論文加值系統

本研究利用電沉積的方式，於鈦金屬基材表面沉積奈米金微粒，並於其上固定精胺酸─甘胺酸─天門冬胺酸─半胱胺酸胜肽序列。使用薄膜X光繞射儀、場發掃描式電子顯微鏡、高解析度X光光電能譜儀、電化學阻抗分析法及循環伏安法對鍍有奈米金之鈦基材表面及胜肽結合效果進行性質分析。實驗結果顯示使用電沉積的方式，可在鈦基材表面沉積上奈米金顆粒，於不同電位下進行電沉積後，便可得到粒徑大小分別約為60 nm、110 nm及80 nm之奈米金顆粒。其相組成屬於多晶型，結晶面包括（111）、（200）、（220）及（311）。X光光電能譜儀結果則顯示胜肽可藉由自組裝的方式被固定於鍍有奈米金之鈦基材上。證明此種表面修飾法可作為胜肽或生物分子固定之替代方法。MG63類骨母細胞貼附實驗顯示所披覆上之RGDC，對於細胞貼附狀況沒有明顯的效果。

The objective of the study was to evaluate the feasibility of nano-structured Au deposited onto titanium substrates using electrodeposition. After which, immobilization of Alginine–Glycine–Aspartate–Cysteine (RGDC) peptide onto nanogold-coated titanium surface was further performed for implant applications. Characterization of the Au nano-particles electrodeposited Ti and peptide immobilization were performed using a thin film X-ray diffractometer (TFXRD), field emission scanning electron microscope (SEM), and high resolution X-ray photoelectron spectroscopy (XPS), in addition to electrochemical methods including electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The results showed that Au nano-particles could be easily electrodeposited on titanium surface. The mean particles size of 60 nm, 110 nm and 80 nm would be obtained following electrodeposition at different applied potential, respectively. XRD patterns indicated a polycrystalline orientation of the gold phase. The characteristic peaks were ascribed to (111), (200), (220), and (311) crystal faces. XPS spectrum illustrated that the RGDC peptide could be successfully immobilized on nano-gold-deposited Ti substrates via self-assembly of Au-S bonding. This procedure might be used as an alternative way for peptide or other bioactive molecules immobilization. However, the results of MG63 osteoblast-like cells cultured onto RGDC-Au-coated titanium surface showed that it did not benefit for cells attachment.

總目錄
誌謝 I
中文摘要 II
Abstract III
總目錄 IV
表目錄 VI
圖目錄 VII
第一章 文獻回顧及理論基礎 1
1-1 生醫材料 1
1-1-1 基本性質 1
1-1-2 骨修復應用 2
1-2 鈦金屬 3
1-2-1 鈦金屬表面修飾 5
1-3 胜肽 8
1-4 金金屬 8
1-4-1 奈米金於生醫工程之應用 9
1-4-2 奈米金製備法 9
1-4-3 奈米金之固定 10
1-4-4 電沉積製備奈米金屬顆粒 11
1-5 研究動機與目的 11
第二章 材料與方法 13
2-1 奈米金製備 13
2-2 奈米金定性分析 13
2-2-1 微結構觀察 13
2-2-2 相組成分析 13
2-2-3 電化學定性分析 14
2-3 RGD peptide披覆後表面化學成分分析 14
2-4 細胞貼附之評估 15
2-4-1 細胞培養 15
2-4-2 細胞貼附 15
第三章 結果與討論 17
3-1 奈米金 17
3-1-1 四氯金酸於純鈦表面還原行為 17
3-1-2 微結構觀察 19
3-1-3 相組成 22
3-1-4 電化學循環伏安法分析 24
3-1-5 電化學阻抗分析 26
3-2 RGDC披覆 28
3-2-1 XPS分析 28
3-2-2 細胞貼附 31
第四章 結論 33
第五章 參考文獻 34

表目錄
表1、鈦金屬之物理性質 4
表2、鈦及其合金表面修飾法 7

圖目錄
圖1、鈦金屬於不同電解液內進行CV結果 18
圖2、不同電沉積電位及時間後之表面微結構 20
圖3、電沉積後之表面成分分析 21
圖4、電沉積奈米金顆粒粒徑大小 21
圖5、電沉積處理後之TFXRD結果 23
圖6、不同電沉積參數之CV圖 25
圖7、不同電沉積參數之Nyquist圖 27
圖8、有無電沉積處理之鈦披覆上RGDC胜肽之XPS全譜圖 29
圖9、有無電沉積處理之鈦披覆上RGDC胜肽之S2p細部掃描 30
圖10、RGDC披覆後細胞貼附結果 32

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