臺灣博碩士論文加值系統

維生素C棕櫚酸酯廣泛的被使用在皮膚製劑及化粧品中，在配方中的穩定性不佳。本研究是利用固體脂質包覆將維生素C棕櫚酸脂使其粒子奈米化，並評估其物理及化學安定性。固態脂質使用鯨蠟醇棕櫚酸酯，分別包覆不同濃度的維生素C棕櫚酸酯；及在配方中分別添加了離子螯合劑乙二胺四乙酸二鈉(EDTA．2Na)及抗氧化劑維他命E。研究結果顯示，經由高壓均值後可得到平均粒徑200 nm左右，多分散指數皆小於0.2的奈米微粒，而不同濃度維生素C棕櫚酸脂固態脂質奈米粒的包埋率在50.01％以上。利用穿透式電子顯微鏡觀察所形成的固態脂質奈米粒形狀為球形。在放置76天後，粒徑沒有太明顯的增加，約增加了7％。添加乙二胺四乙酸二鈉及維他命E皆可提高維生素C棕櫚酸酯固態脂質奈米粒的穩定性。

Ascorbyl Palmitate (AP) is widely used in pharmaceutical and cosmetic preparations, but it is not stable in formulation. The purpose of the present work is to study the solid lipid nanoparticles(SLNs) encapsulation with ascorbyl palmitate. Cetyl palmitate was selected as the lipid matrix. Several factors were investigated in this study, including (a) the loading capacities of AP in SLNs. (b)the effect of additation with ethylenediaminetetraacetic acid (EDTA). (c) the effect of additation with Vitamin E (Vit E). The result showed that these SLNs diameter are around 200 nm with a narrow polydispersity index (P.I) lower than 0.2 after high pressure homogenization. The loading capacities were over 50％. The transmission electron microscopy (TEM) images showed these SLNs are spherical. The addition of EDTA obviously increase the loading capactity. The storage stability were also evaluated over 70 days. The diameter of SLNs remained around 200 nm. The addition of EDTA and Vit E can increase the SLNs stability.

中文摘要……………………………………………………………Ι
英文摘要……………………………………………………………Ⅱ
謝致…………………………………………………………………Ⅲ
目錄…………………………………………………………………Ⅳ
圖目錄………………………………………………………………Ⅶ
表目錄………………………………………………………………Ⅷ
第一章 前言……………………………………………………………1
1.1研究動機及目的……………………………………………………1
1.2研究架構……………………………………………………………2
第二章文獻探討………………………………………………………4
2.1 SLNs………………………………………………………………4
2.1.1 前言……………………………………………………………4
2.1.2 SLNs的組成……………………………………………………5
2.1.3 SLNs的製備……………………………………………………6
2-1-4 SLNs在化粧品的應……………………………………………9
2.2 維他命C棕櫚酸酯………………………………………………11
第三章 實驗材料及設備及方法……………………………………13
3.1 實驗材料…………………………………………………………13
3.2 實驗儀器…………………………………………………………14
3.3 SLNs製備 …….…………………………………………………17
3-3-2 實驗配方………………………………………………………17
3.4 製程溫度對維他命C棕櫚酸酯影響………………………………18
3.5 粒徑與界面電位測量……………………………………………18
(a) 粒徑測量…………………………………………………………19
(b) 界面電位測量……………………………………………………19
3.6 總量回收率和包埋率測定………………………………………19
3.7 安定性評估………………………………………………………20
(a) 物理安定性評估…………………………………………………20
(b) 化學安定性評估…………………………………………………21
3.8 DSC 熱分析與穿透式電子顯微鏡………………………………21
第四章 結果與討論………………………………………………23
4.1 維他命C棕櫚酸酯界面活性劑溶液的影響在75℃之穩定性評…22
4.2 不同循環次數對於粒徑的影響…………………………………25
4.3 SLNs包覆維他命C棕櫚酸酯之粒徑及界面電位…………………27
4.4 掃描式熱差分析儀………………………………………………29
4.5 穿透式電子顯微鏡………………………………………………32
4.6 總量回收率………………………………………………………34
4.7 包埋率……………………………………………………………36
4.8 物理安定性 ………………………………………………………38
4.9 化學安定性………………………………………………………43
第五章 結論………………………………………………………50
參考文獻………………………………………………………………51

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