臺灣博碩士論文加值系統

目的：本研究將依據衛生福利部食品藥物管理署 (2016) 「健康食品之護肝保健功效評估方法」之規範，探討藤黃果與青咖啡豆萃取物複方對於非酒精性肝損傷的功效評估。方法：實驗樣品為藤黃果與青咖啡豆萃取物之複方，以雄性C57BL/6小鼠進行高脂誘發肝損傷動物模式，組別分為一般控制組 (Normal control)、高脂誘發肝損傷對照組 (HFD)、1倍複方劑量組 (HFD -1X)、2倍複方劑量組 (HFD -2X)與5倍複方劑量組 (HFD -5X)，分別收集第0、1、8、11、18周之血液中指標（空腹血糖、總膽固醇、三酸甘油脂、天門冬胺酸轉胺酶、丙氨酸氨基轉移酶)，並在第18周犧牲小鼠，收集肝臟進行脂質含量分析以及組織切片觀察與評分，最後使用one-way ANOVA利用Duncan's test 了解不同處理間是否具有差異，以P <0.05 代表具有統計上顯著意義，為了瞭解不同劑量之間是否有劑量效應，則用趨勢分析 (Trend analysis) 並以Cochran-Armitage test進行檢定。結果：（一）補充複方組18周後相對於高脂控制組體重顯著較低。（二）補充複方組18周後相對於高脂控制組血液中TG與TC濃度顯著較低。（三）補充複方組18周後相對於高脂控制組血液中ALT與AST濃度顯著較低。（四）補充複方組18周後相對於高脂控制組空腹血糖相對控制組顯著較低。（五）肝臟組織切片分析補充複方組18周後相對高脂控制組相對有較少的肝臟脂肪細胞。結論：藤黃果與青咖啡豆萃取物而成之複方對於非酒精性肝損傷具有預防保健之效果。

Purpose: This study will be based on the Food and Drug Administration of the Ministry of Health and Welfare (2016) “Method for assessing the efficacy of liver health care for healthy foods”. To evaluate the efficacy of the combination of Garcinia Cambogia and Green Coffee Bean Extract (GCGCBE) for non-alcoholic liver injury. Methods: The experimental sample is a combination of Garcinia Cambogia and green coffee bean extract, and animal model of high fat-induced liver injury in male C57BL/6 mice. The groups were divided into normal control group, high-fat-induced liver injury control group (HFD), 1-fold dose group (HFD-1X), 2-fold dose group (HFD-2X), and 5-fold dose group (HFD-5X). Daily doses of the corresponding doses were collected for blood levels at 0, 1, 8, 11, and 18 weeks (Fasting blood glucose (FBG), Total Cholesterol (TC), Triglyceride (TG), Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST), and sacrificed mice at week 18, livers were collected for lipid content analysis and tissue section observation and scoring. Use one-way ANOVA to understand whether there is a difference between treatments. P < 0.05 represents statistically significant. To understand whether there is a dose effect between different doses, use Trend analysis is checked. Results: The results of this study were: (1) After 18 weeks of supplementation, the body weight was significantly lower relative to the high fat control group. (2) The supplemental combination group was significantly lower than the high fat control group TG, TC, ALT, AST and FBG. (3) The supplemental combination group had relatively few liver fat cells in the relatively high-fat control group. Conclusion: The combination of GCGCBE has preventive and health effects on non-alcoholic liver injury.

中文摘要
英文摘要
目次
表次
圖次
第一章 緒論
第一節 研究背景與動機
第二節 研究目的
第三節 研究假設
第二章 文獻探討
第一節 血脂
第二節 非酒精性脂肪肝與肝損傷
第三節 高脂血症與非酒精性脂肪肝的關係
第四節 藤黃果萃取物與高血脂
第五節 青咖啡豆萃取物與高血脂
第三章 研究方法
第一節 實驗測試樣品
第二節 實驗動物之飼養與分組
第三節 試驗樣品投予方式
第四節 實驗分組
第五節 實驗流程
第六節 數據與樣本收集
第七節 分析項目
第八節 統計分析
第四章 結果與討論
第一節 結果
第二節 討論
第五章 結論
第六章 參考文獻
中文參考文獻
外文參考文獻
附錄
附錄一 動物實驗審查同意書
附錄二 SGS檢驗報告書- SGS FA/2017/65804

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