臺灣博碩士論文加值系統

口腔癌細胞多發生在舌部，其次為粘膜、牙齦、口部地方。基於此，我們認為如果能開發口腔癌治療貼片，將治療藥物包覆在基材中，直接覆蓋在口腔癌病灶處，可以是一種非侵入性的治療方式，同時具有保護腫瘤切除後傷口。本實驗利用結冷膠 (Gellan gum ; GG) 研製出一種新的具有可降解性且具經濟效益的薄膜，基於文獻回顧我們認為Curcumin (薑黃素; CM) 及 Clioquinol (氯碘仿; CQ) 抗癌機轉可應用於口腔癌治療，研究結果發現 Gellan gum 薄膜具有良好的機械性質，薄膜經交聯後，在含水量和含膠量的實驗結果顯示， CM 及 CQ 的存在下不影響交聯結果， GG-CM 的含水量為 97.6% 及 93% ，含膠量為 87% 及 83.5%；GG-CQ的含水量為 97.6% 及 93.5%，含膠量為 87.3% 及 83.5%。經交聯後的薄膜在 28 天的降解試驗後分別是 GG-CM 91.8% 及 88.6% ； GG-CQ 91.4% 及 90.1%。經交聯後的薄膜在 50 天累積藥物釋放率分別可達到 GG-CM 89.6% 及 86.5% ；GG-CQ 86.8% 及 82.4%。動物模型建立方面，經由 14 週的 DMBA 誘導，結果顯示，可見頰囊外觀具有明顯的表皮變異，除外觀可見到局部脫毛，且有出血、潰瘍及紅斑病變現象，亦可見到顆腫瘤生成。

Oral cancer frequently happens at tongue, mucous, gingiva and oral area. Base on this, we consider that developing the patch containing the drug as one of the basic materials and covering on the affected region of the oral cancer can be a non-invasive treatment and also protect the wound that causes by the tumor-cutting operation. We used the Gellan gum to develop a new patch which has degradability and also economic in our experiment. According to the previous, Curcumin and Clioquinol can be used to apply on the treatment of the oral cancer. We have found that Gellan gum gets the fine mechanical property in the result. And after the cross-linking process, the experimental result of gel content and water content showed that the existence of CM and CQ don''t affect the cross-linking consequence. The water content of GG-CM are 97.6% and 93% and the gel content are 87% and 83.5%. The water content of GG-CQ are 97.6% and 93.5% and the gel content are 87.3% and 83.5%. The cross-linked patch showed the GG-CM 91.8% and 88.6%,GG-CQ 91.4% and 90.1% in the degradability test after 28 days. The cross-linked patch showed the GG-CM 89.6% and 86.5%, GG-CQ 96.6% and 82.4% in the drug accumulative release test after 50 days. In the animal model, used DMBA induced after 14 weeks, the results showed that the appearance of the cheek pouch has a significant variation in the epidermis, the appearance can be seen localized hair removal, and bleeding, ulcers, lesions of erythema and tumorigenesis.

論文電子檔著作權書 ................................................................................. i
論文口試委員審定書 ................................................................................ ii
誌謝 ........................................................................................................... iii
中文摘要 ................................................................................................... iv
英文摘要 .................................................................................................... v
目錄 ........................................................................................................... vi
圖目錄 ....................................................................................................... ix
第一章 緒論
1. 口腔癌..……………..………………………………………………... 1
2. 結冷膠簡介………………………………….……………………… 11
3. 薑黃素簡介………………………………………………………..... 16
4. Clioquinol簡介……………………………………………………... 19
5. Glucosamine 簡介…………………………………………….……. 22
6.　藥物釋放……………………………………………………………..24
7.　研究動機……………………………………………………......…... 35
第二章 材料與方法
1. 實驗材料與配製方法…………………………………......………... 36
2. 口腔癌治療貼片…………………………………….…………........ 39
　2-1.　Gellan gum-CM薄膜及藥物包覆製備.……………………….. 39
　2-2.　Gellan gum-CQ薄膜及藥物包覆製備.……………………….. 39
　2-3.　Gellan gum-CM/CQ薄膜交聯作用.…………………….…….. 40
　2-4.　水膠含水率(water content) .…………………………….…….. 40
　2-5.　水膠含膠率(gel content) .………………...………...…………. 40
　2-6.　薄膜拉伸試驗.………………………………………...…….…. 41
　2-7.　Gellan gum-CM/CQ薄膜體外降解試驗.……..………….……. 41
　2-8.　製作Curcumin(CM)之標準曲線.……………..……….………. 41
　2-9.　製作Clioquinol(CQ)之標準曲線.………………….………….. 42
　2-10.　體外藥物釋放試驗.…………………………….…………….. 42
3. 建立動物模型..................................................................................... 42
第三章 結果
1. Gellan gum-CM / CQ薄膜巨觀圖.………………………………..... 44
2. Gellan gum－Curcumin/Clioquinol薄膜的含水量.……………...… 46
3. Gellan gum－Curcumin/Clioquinol薄膜的含膠量.………………... 48
4. 結冷膠包覆薑黃素及 Clioquinol 薄膜拉伸試驗結果.…………... 50
5. Gellan gum-CM/CQ薄膜體外降解試驗.…………………………... 52
6. 交聯前後GG-CM/CQ的FTIR分析結果.…………………………... 54
7. 繪製Curcumin及Clioquinol之標準曲線.…………………………... 60
8. 體外藥物釋放試驗.………………………………………………… 63
9. 動物模型的建立.………………………………………………….... 70
第四章
討論………………………………………………………….……….….. 72
結論………………………………………………………….……….….. 75
參考文獻………………………………………………….……………... 76

財團法人台灣癌症基金會
　(http://www.canceraway.org.tw/cancerpageshow.asp?IDno=423)
行政院衛生福利部 (http://www.mohw.gov.tw/cht/DOS/Statistic.aspx?f_list_no=312)
曾莉婷，Clioquinol與銅離子合併治療口腔癌引發Caspase非依賴性細胞凋亡和細胞生長停滯，碩士論文，醫學檢驗生物技術學研究所，高雄醫學大學，中華民國一百零二年。
林宜美，幾丁質摻盒具乳酸酯微粒於藥物釋放系統之研究，碩士論文，化學工程與材料工程研究所，國立中央大學，中華民國九十一年六月
楊國明，藥物釋放之親疏水性乙基纖維素/羥丙基纖維素摻合微粒的製備與其藥物釋放之研究，碩士論文，化學工程學系，國立成功大學，民國九十五年三月二十四日。
魏宗平，PLGA微球包覆Rapamycin與蠶絲蛋白/明膠形成複合薄膜之藥物釋放探討，碩士論文，化學工程與材料工程研究所，國立台北科技大學，中華民國一百零一年六月。
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