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研究生:黃國展
研究生(外文):Huang, Kuo-Chan
論文名稱:合成磁性奈米複合物並做為MRI/CT雙功能影像運用
論文名稱(外文):Synthesis of magnetic core–shell Fe3O4–Au nanoparticle for MRI/CT Hybrid Imaging Application
指導教授:蔡長書蔡長書引用關係
指導教授(外文):Tsai, Chang-Shu
口試委員:丁慧枝、楊天賜、許博翔、蔡長書
口試委員(外文):DING, HUI-ZHI、Yang, Tien-Syh、Hsu, Po-Hsiang、Tsai, Chang-Shu
口試日期:2016-07-28
學位類別:碩士
校院名稱:慈濟科技大學
系所名稱:放射醫學科學研究所
學門:醫藥衛生學門
學類:醫學技術及檢驗學類
論文種類:學術論文
論文出版年:2017
畢業學年度:105
語文別:中文
論文頁數:105
中文關鍵詞:磁性奈米粒子、奈米複合MRI/CT 對比劑、葡聚醣、超順磁性
外文關鍵詞:magnetic nanoparticles、nanohybride MRI/CT contrast agent、dextran、superparamagnetic property
相關次數:
  • 被引用被引用:0
  • 點閱點閱:241
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中文摘要
磁性奈米粒子近年來不斷的創新以及多元應用,目前已廣泛運用在醫學影像、熱治療藥物、藥物載體、特異性檢測藥物等方面;本研究為探討製備 Fe3O4 磁性奈米粒子,使用葡聚醣(Dextran)做為此磁性奈米粒子之介面活性劑,進行批覆後,利用其表面修飾出之胺基與金奈米粒子產生鍵結,以形成 Fe-Au 奈米複合 MRI/CT 對比劑。
本實驗以化學共沉澱法製備 Fe3O4 磁性奈米粒子,並且在製備時改變製備條件,以製備出較小粒徑之 Fe3O4 磁性奈米粒子,並以低分子量之 Dextran(MW =9000-11000)進行批覆 Fe3O4 作為磁性奈米粒子之介面活性劑,並透過 One-step process 以及 Two-step process 兩種不同鍵結方式在其磁性奈米粒子表面鍵結金奈米粒子;而由實驗結果顯示以 Two-step process 鍵結金奈米粒子在元素分析中具有較高含量的奈米金粒子,並且在飽和磁化率的分析中比 One-step process 鍵結方式具有較高的飽和磁化率,進一步將磁性奈米複合物置於 3T MRI 以及256切電腦斷層掃描進行體外影像品質探討發現,以 Two-step process 合成出的磁性奈米複合物在 3T MRI 的 T2 加權影像中其訊號強度為 42.2s-1 高於 One-step process 的 7.93 s-1,但在影像中皆有顯著的訊號抑制效果;在256切電腦斷層影像掃描結果也顯示透過 Two-step process 合成出的磁性奈米複合物在濃度達到 50 ug/mL 對 X 光的衰減來到了 131.9 HU 略高於市售 Omnipaque 碘造影劑的 123.3 HU,而相對於透過 One-step process 合成出的磁性奈米複合物則未看 到對於 X 光具有明顯衰減。
而將奈米複合物置於常溫下保存時不論是單一磁性奈米粒子亦或 是透過 One-step process 以及 Two-step process 製備出的磁性奈米複合物皆有良好的穩定性以及分散性,在室溫環境放置超過90天依舊未發現沉澱以及團聚現象產生。

Abstract

To date, a wide variety of magnetic nanoparticles have been produced, differing in size and type of coating material,including dextran, citrate, starch,albumin, silicones and polyethyleneglycol. The diversified usage of magnetic nanoparticles were particularly in the biomedical applications including MRI contrast agent, hyperthermia, PET-MR contrast agent and drug delivery. Among the developed nanoparticles systems,superparamagnetic iron oxide nanoparticles have been extensively explored as a negative contrast agent for T2-weighted magnetic resonance imaging applications.

In this study, we used the chemical co-precipitation method to prepare the superparamagnetic iron oxide nanoparticles, and coated with: good biocompatibility, can reduce the biological toxicity and make the nanoparticles more stable in human body and chemically stable effect. The selected of low molecular weights dextran as a magnetic nanoparticles interface active agent, and combined with the gold ion (of Au3+) onto the iron oxide nanoparticle’s surfaces via iterative hydroxylamine seeding, the combination of the two differentiation magnetic pole has potential for use in MRI/CT contrast agent applications.

The MR and CT imaging data reveal that the formed Fe3O4@Au nanohybride have a relatively high r2 relaxivity (42.2s-1) and good X-ray attenuation property, which enables their uses as contrast agents for MR and CT imaging of water phantom. The Fe3O4@Au nanohybride developed via the co-precipitation approach may have promising potential for the dual-mode MR/CT.

目次 致謝 中文摘要 英文摘要
=====================================================================
第一章 緒論


1.1 前言
1.2 奈米生物醫學
1.3 奈米粒子之基本特性(物理、化學、光學與生理特性)
1.4 奈米粒子製備
1.5 用於披覆磁性奈米粒子之界面活性劑介紹
1.6 核磁共振與造影原理
1.7 電腦斷層掃描原理
=====================================================================
第二章 文獻回顧


2.1 磁性奈米粒子藥代動力學
2.2 核磁共振造影劑
2.3 金奈米粒子影響
2.4 金奈米粒子電價選擇
2.5 鐵金奈米複合物應用
2.6 研究目的
2.7 研究架構
=====================================================================
第三章 實驗材料與方法


3.1 實驗藥品
3.2 實驗儀器
3.3 實驗流程
3.4 製備方法與步驟
3.5磁性奈米粒子之物理與化學性質鑑定


=====================================================================
第四章 結果與討論


4.1合成四氧化三鐵磁性奈米粒子
4.2 合成金奈米粒子

4.3 合成鐵/金奈米複合粒子
4.4 離心純化
4.5 奈米粒子粒徑以及穩定度分析
4.6 奈米複合粒子EDS元素分析
4.7 奈米複合粒子表面電漿共振分析
4.8 奈米複合粒子溶液介電位分析
4.9 磁性奈米粒子表面官能基以及磁化率分析
4.10 體外核磁共振訊號變化
4.11 體外電腦斷層掃瞄影像


=====================================================================
第五章 結論


5.1 結論
5.2 磁性奈米粒子粒徑與核磁共振以及電腦斷層造影影像探討
5.3 總結


=====================================================================
第六章 未來展望


6.1 未來展望
=====================================================================
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