臺灣博碩士論文加值系統

隨著工業與科技的快速發展，過程中雖然便利了人們的生活，同時伴隨著眾多的環境污染，對地球環境產生了極大的影響，其中包括工業廢棄物、水污染、空氣污染、土壤污染及噪音等污染，現今身邊的各種環境問題漸漸受到重視。
本研究採用上流式厭氧污泥床(Up-flow Anaerobic Sludge Blanket, UASB)所馴養之厭氧污泥進行試驗，分別額外添加4 g/L丁酸，探討丁酸之代謝路徑與發電效率，其次應用代謝通量分析代謝路徑中反應物、中間產物等濃度之變化及反應速率，加以探討其代謝通量。結果發現，額外添加丁酸時，其丁酸濃度變化由起始4138.89 mg/L，最終變為3086.72 mg/L，丁酸利用率為34%。本研究所探討之丁酸代謝路徑，起始反應物為丁酸，最終產物為戊酸，參與反應物質有30項。結果顯示，丁酸代謝路徑中各物質之代謝通量，第1天時為0.0038至3.3 g/day之間，至第6天時，降為0.0014至1.21 g/day之間。本研究依據實驗所測得之丁酸濃度，帶入有機酸分解菌之量化公式後得知，丁酸分解菌起始濃度為8.91 mg/L，並逐天上升，至第6天時為87.15 mg/L。在添加丁酸實驗第0天之中，電流可達最高至84 μA，添加丁酸實驗之平均電流以49.1±17.7 μA。本研究添加丁酸實驗之電壓，在第0天可測出最高電壓140.2 mV，且平均電壓79.8±33.5 mV。

With instant development of industry and technology, people's life is convenient, but numerous environmental pollutions have a severe effect on the earth's environment, including industrial waste, water pollution, air pollution, soil pollution and noise pollution. At present, all kinds of environmental problems are paid increasing attention to.
This study uses the anaerobic sludge reclaimed by Up-flow Anaerobic Sludge Blanket (UASB) for experiment, which is mixed with 4 g/L butyric acid to discuss the metabolic pathway and generating efficiency of butyric acid. Secondly, the concentration changes and reaction rate of reactants and intermediate products in the metabolic pathway are analyzed by using metabolic flux, and the metabolic flux is discussed. The results show that after the addition of butyric acid, the butyric acid concentration changes from initial 4138.89 mg/L to final 3086.72 mg/L, the butyric acid utilization rate is 34%. On the metabolic pathway of butyric acid discussed in this study, the initial reactant is butyric acid, the end product is valeric acid, and there are 30 reacting substances. The results show that the metabolic flux of various materials on the metabolic pathway of butyric acid is 0.0038 to 3.3 g/day on Day 1, that decreases to 0.0014 to 1.21 g/day on Day 6. The experimentally measured butyric acid concentration is substituted in the quantitative equation for organic acid decomposing bacteria, the initial concentration of butyric acid decomposing bacteria is 8.91 mg/L, and it increases day after day, it is 87.15 mg/L on Day 6. On Day 0 of experiment with additional butyric acid, the current is maximum 84μA, the average current is 49.1±17.7μA in the experiment with additional butyric acid. The maximum voltage is 140.2 mV on Day 0 of experiment with additional butyric acid, the average voltage is 79.8±33.5 mV.

目錄
第一章 緒論 1
第一節 研究背景與動機 1
第二節 研究目的 2
第三節 名詞解釋 2
第二章 文獻回顧 5
第一節 厭氧微生物 5
第二節 厭氧醱酵酸化反應機制 7
第三節 厭氧微生物反應器 9
第四節 酸生成物之反應式 11
第五節 微生物燃料電池產電原理 12
第六節 代謝通量分析原理 14
第七節 ADM1應用於厭氧槽中微生物功能族群之研究 19
第三章 研究方法 25
第一節 研究架構 25
第二節 研究流程 26
第四節 代謝通量分析 33
第五節 微生物功能族群量化 34
第四章 結果與討論 35
第一節 添加有機酸之厭氧反應槽操作情形 35
第二節 UASB厭氧槽中有機酸之微生物功能族群量化 40
第三節 酸分解菌與產電效能之影響 42
第四節 厭氧槽中有機酸代謝路徑之代謝通量分析 47
第五節 單位有機酸起始濃度及單位有機酸分解菌濃度下之代謝通量 59
第六節 能量載體及離子之代謝通量 69
第五章 結論與建議 71
第一節 結論 71
第二節 建議 72
參考文獻 73

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