臺灣博碩士論文加值系統

本實驗室先前所發展的動態光罩快速成型系統中，使用不需添加光交聯劑即可光固化成型之PCL材料，將其添加光起始劑並且溶於丙酮，經光固化可成功地製作出所需支架。但由於無光交聯劑的關係，使得製作出來的支架強度不足。故本研究將添加市售的光交聯劑PEG-acrylate來彌補材料強度之不足，並且調整動態光罩快速成型系統，使其能與材料系統相互搭配，提升整體精度，製作出3D多孔性組織工程支架。
本研究將可光固化成型之PCL材料與市售之光交聯劑PEG-acrylate，以6:4、7:3及8:2三種比例進行光固化測試。此三種比例之材料，測得其抗拉強度分別為0.52、0.3、0.25MPa，與先前研究之0.13MPa相較，有大幅之提升；細胞培養結果，亦可見細胞能於其上貼附及生長。由於PEG-acrylate光敏性較強，成型系統加入偏光鏡、套筒以及鏡頭，以降低光強度及延長曝光時間，除可避免過曝現象之外，亦可提高支架製作之精度。三種比例之材料應用於製作單層大孔徑支架，誤差可達2%以內；其中8:2的材料能夠成功地製作出重現性高的150μm孔洞之單層支架，誤差低於5%。並以此精度最佳之材料比例(8:2)製作3D支架，透過網狀偏移的支架設計概念，可堆疊製作出孔徑為100μm的3D多孔性組織工程支架。

In the previous research in our laboratory, the photo-curable PCL, mixing with photo-initiator, was dissolved in the acetone and photo-polymerized to fabricate tissue engineering scaffolds by a Dynamic Masking Rapid Prototyping System. However, without cross-linking binders, the strength of the cured PCL is not satisfying. Therefore, in this research, commercially available PEG- acrylate was adopted as binder in the material system to improve the strength, the Dynamic Masking RP system was modified for the material to reach better process accuracy, and 3D porous scaffolds were fabricated.
The materials with three different proportions of photo-curable PCL and PEG-acrylate (6:4, 7:3, and 8:2) were investigated in this study. The tensile strength of cured materials were 0.52, 0.3, and 0.25 MPa respectively, which are higher than the strength of 0.13 MPa in the previous research without adding PEG-acrylate. Cell culture results also showed the evidences of cells attachment and growth. Due to the better photo-sensitivity of PEG-acrylate, polarizer, sleeve, and lens were added to the Dynamic Masking RP system to reduce light intensity and to prolong the exposure time. This can not only avoid over-curing, but also improve the process accuracy. Three materials were applied to fabricate single layer scaffold with larger pore size and the errors were within 2%. In particular, when utilizing material with proportion of 8:2, highly repeatable single layer scaffold with 150μm pore size was successfully fabricated with the error less than 5%. This best material recipe was further used to generate 3D scaffolds with 100μm pore size through offsetting grid pattern in the alternating layer.

摘要 .................................................... 4
Abstract ................................................ 5
誌謝 .................................................... 6
目錄 .................................................... I
圖目錄 ................................................. IV
表目錄 ................................................. IX
第一章 緒論 ............................................. 1
1.1 前言 ............................................. 1
1.2 研究背景與動機 ................................... 2
1.3 研究目的和方法 ................................... 3
1.4 論文架構 ......................................... 4
第二章 文獻探討 ......................................... 5
2.1 組織工程(Tissue Engineering)介紹 .................. 5
2.1.1 支架材料之特性 .............................. 7
2.1.2 高分子生醫材料 .............................. 8
2.1.3 傳統支架製備方法 ........................... 15
2.2 應用快速原型技術製造組織工程之支架 ............... 19
2.2.1 快速原型加工原理 ........................... 19
2.2.2 快速原型技術應用於組織工程支架之製造 ........ 21
第三章 生醫材料調配與性質檢測 ........................... 32
3.1 實驗藥品與設備 .................................. 32
3.2 材料系統介紹 .................................... 33
3.2.1 可交聯PCL 合成 ............................. 34
3.2.2 光起始劑 ................................... 36
3.2.3 光交聯 ..................................... 38
3.2.4 PCL(聚己內酯) --活性寡聚體 ................. 40
3.2.5 光聚合生醫材料 ............................. 40
3.3 材料性質檢測 .................................... 42
3.3.1 拉伸詴驗 ................................... 42
3.4 細胞培養與生物相容性檢測 ......................... 51
3.4.1 實驗藥品 ................................... 51
3.4.2 細胞培養 ................................... 52
3.4.3 倒立式顯微鏡觀察細胞生長情況 ............... 55
第四章 系統簡介與機構改善 .............................. 59
4.1 生醫動態光罩快速成型系統 ........................ 59
4.1.1 上下照式成型系統差異比較 .................... 59
4.2 下照式生醫動態光罩快速成型系統 ................... 61
4.2.1 動態光罩控制軟體 ........................... 62
4.3 系統相關機構變更 ................................ 70
4.3.1 光學系統變更 ............................... 70
4.4 參數設定與精度檢測 .............................. 77
4.4.1 參數設定 ................................... 77
4.4.2 精度檢測 ................................... 79
4.4.2.1 量測系統介紹 .......................... 79
4.4.2.2 支架精度量測 .......................... 81
第五章 製作3D 組織工程支架 .............................. 85
5.1 加工流程 ........................................ 85
5.2 單層微小孔徑支架製作 ............................ 86
5.3 3D 偏移支架設計與製作 ............................ 92
5.3.1 2D pattern 的設計........................... 93
5.3.2 3D 偏移支架的製作與量測 ..................... 96
5.4 研究成果比較 ................................... 101
第六章 結論與未來研究方向 ............................. 104
6.1 結論 ........................................... 104
6.2 未來工作 ....................................... 105
參考文獻 .............................................. 106

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