臺灣博碩士論文加值系統

近這幾年來，許多的專家學者與環保人士皆相當關注仿雌激素對兩生類所造成的影響。為了瞭解仿雌激素物質的影響，本研究選用澤蛙 (Rana limmocharis) 作為模式生物，注射雌激素 (17β-estradiol) 0.1, 1 μg/L 及雙酚A (Bisphenol-A) 0.1, 0.5 μg/L 七天及十四天後，量測肝臟/體重比指數 (hepatosomatic index, HSI)、生殖腺/體重比指數 (gonadosomatic index, GSI) 及卵黃前質蛋白 (vitellogenin, VTG) 的含量，來瞭解仿雌激素物質對蛙類的影響。本研究結果顯示：(1)在注射 17β-estradiol 十四天後，其 vitellogenin 誘發率高於對照組的 3 倍；而注射 Bisphenol-A 十四天後，其 vitellogenin 誘發率為相對對照組誘發倍數的 2 倍。(2)肝臟及生殖腺指數部份，注射 17β-estradiol 後明顯會使肝臟體重比指數下降，Bisphenol-A 部分則會有指數上升的現象；而生殖腺指數，注射 17β-estradiol 之生殖腺指數較注射 Bisphenol-A 具有明顯下降的情況，且隨著時間的增長，其影響的現象更為明顯。因此，從上述訊息得知，17β-estradiol 與 Bisphenol-A 的確會使澤蛙體內 vitellogenin 產生誘發，可由此推斷 Bisphenol-A 具有仿雌激素的影響。

In the past decad, many researchers are concerned about the impacts of xenoestrogen on amphibian. In order to study the effects of estrogenic substances on amphibian, we injected Rana limmocharis with 17β-estradiol (0.1 and 1.0 μg/L) and bisphenol-A (0.1 and 0.5 μg/L). The hepatosomatic index (HSI), gonadosomatic index (GSI) and vitellogenin (VTG) were measured after 7 and 14 days. The results of this study are as followed : 1. animals injected with 17β-estradiol after 14 days, there vitellogenin induction were 3-fold higher than those in the control group ; for animals injected with biphenol-A after 14 days, their vitellogenin induction has a 2-fold increase compared with the control group. 2. in animals injected with 17β-estradiol could significantly inhibit the liver growth, however, animals treated with biphenol-A could significantly increase the weight of the liver; animals injected with 17β-estradiol has obviously decreased in gonad weight than biphenol-A did, and with longer exposure period, the effect was more significant. Both 17β-estradiol and bisphenol-A could induce the vitellogenin in R. limmocharis. We have concluded that the bisphenol-A can mimic estrogenic effect on R. limmocharis.

目錄
摘要 I
Abstract II
誌謝 III
目錄 V
表目錄 VII
圖目錄 VIII
第一章 前言1
第二章 文獻回顧3
一、環境荷爾蒙3
二、環境荷爾蒙物質-雙酚A簡介5
三、檢測環境荷爾蒙之生物指標 (biomarker)8
四、澤蛙 (Rana limmocharis) 型態特徵 10
第三章 研究方法與材料11
一、實驗動物來源與蓄養11
二、實驗進行流程11
三、卵黃前質蛋白分析12
四、資料分析方法16
第四章 結果與討論17
一、公澤蛙經注射 17β-Estradiol 及 Bisphenol-A 後身長體重之變化17
二、肝臟及生殖腺體重比指標20
三、卵黃前質蛋白誘發反應24
四、討論40
第五章 結論44
參考文獻46
表目錄
表2-1雙酚A的基本物理化學特性6
表4-1注射17β-Estradiol及Bisphenol-A對公澤蛙身長體重之變化影
響19
表4-2注射17β-Estradiol及Bisphenol-A 7 天及 14 天公澤蛙肝臟體重比指數21
表4-3注射17β-Estradiol及Bisphenol-A 7 天及 14 天公澤蛙生殖線體重比指數23
表4-4注射17β-Estradiol及Bisphenol-A 7 天之公澤蛙卵黃前質蛋白誘發濃度38
表4-5注射17β-Estradiol及Bisphenol-A 14天之公澤蛙卵黃前質蛋白誘發濃度39

圖目錄
圖 3-1 Experion Pro260 自動電泳分析儀實驗流程及流程圖15
圖4-1蛋白質分析樣本圖25

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