臺灣博碩士論文加值系統

表面增強拉曼散射(Surface Enhanced Raman Scattering , SERS)主要的增強機制有化學增強機制和電場增強機制。兩個金屬的奈米粒子之間的距離很接近時會產生耦合的作用，粒子中間間隙的地方會有電場增強，稱為熱點。本實驗使用化學還原合成法製備銀奈米粒子溶液，再將銀奈米粒子表面修飾8-mercapto-octanoic acid (MOA)分子，最後使用離心法將修飾過的銀奈米粒子排列成固定間距的陣列。分別使用浸泡或滴乾的方法，讓待測分子Rhodamine 6G (R6G)在樣品上吸附，待測分子會進入銀奈米粒子陣列中的間隙，使分子SERS訊號增強。檢測不同濃度的R6G分子在銀奈米陣列上的SERS訊號強度，發現強度並非隨著濃度提升而持續增強。當R6G密度低於1⤫10^3 (分子個數/ μm^2)時，SERS強度和濃度會呈正相關，推測是位於熱點的R6G分子數量隨密度增加。當R6G密度在高於1⤫10^3 (分子個數/ μm^2)時，SERS強度反而隨密度上升而些微下降。推測是由於表面吸附的R6G分子密度高而造成分子重疊，讓其SERS訊號降低。上述實驗我們以兩種不同波長的拉曼激發光源做量測，量測出來的SERS強度會整體改變，但趨勢變化相似。先前的模擬結果發現，不同激發光波長會影響銀奈米粒子間電場增強的程度。因此SERS訊號隨密度的變化或許可以由熱點的電場增強程度與範圍來解釋。

The main enhancement mechanism of Surface Enhanced Raman Scattering (SERS) is chemical strengthening mechanism and electric field enhancement mechanism. When two metal nanoparticles get very close, they will couple each other. There will be an electric field enhancement between two particles, called a hot spot. In this experiment, the silver nanoparticle solution were prepared by chemical reduction method. After that, the silver nanoparticles were surface-modified with 8-mercapto-octanoic acid (MOA) molecules. Finally, the modified silver nanoparticles were arranged into a fixed-pitch array by centrifugation. Using the method of immersion and titration, the Rhodamine (R6G) molecule is adsorbed on the sample. The R6G molecule enters the gap between the nanoparticles and enhances the SERS signal. The SERS signal intensity of different density of R6G molecules on the silver nanoparticles array was measured. We found that the SERS signal intensity did not increase continuously with the increase of R6G concentration. When the R6G density is lower than 1⤫10^3 (molecule/ μm^2), the SERS signal intensity and concentration will be positive correlation. We assumed that the number of R6G molecules distributed near the hot spots increase as the concentration increase. When the concentration of R6G is higher than 1⤫10^3 (molecule/ μm^2). Instead, the SERS signal slightly decrease with the increase of R6G density. We suppose that high R6G molecule density lead to R6G molecules overlapping. As a result, the SERS signal will be decreased. In the above experiments, we use the Raman excitation light of two different wavelengths as the light source. The measured SERS signal intensity will change overall. However, it will not affect variation trend of the R6G density and the SERS signal intensity. Previous simulation have shown that Raman excitation light of different wavelengths affect the degree of electric field enhancement between silver nanoparticles. Therefore, the variation of the SERS signal with density may be explained by the degree and extent of electric field enhancement of the hot spot.

第一章 緒論 1
第二章 研究原理 9
第三章 研究方法 21
第四章 研究結果與討論 45
第五章 結論 73
文獻參考 75

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