臺灣博碩士論文加值系統

研究資料顯示纖維化在高血壓腎臟病的發病機制和病程中扮演著關鍵的因子。運動訓練保護能改善高血壓腎臟病或腎功能衰竭。然而，運動訓練調節腎臟抗纖維化的作用機制尚不清楚。因此，本篇的目的為研究運動訓練對高血壓引起的腎臟纖維化的效果。實驗使用三十隻六個月大的大鼠，其中十隻WKY為作為對照組，另外二十隻自發性高血壓大鼠 (SHR) 隨機挑選十隻進行為期十二周的跑步機運動訓練。在運動訓練完成後將三十隻大鼠犧牲取出腎臟測量Kidney characteristics，並以Masson’s trichrome staining analysis 測量腎臟的纖維化區域大小 以及以 Western Blotting 測量腎臟纖維化的因子。實驗結果顯示在自發性高血壓老鼠血壓、腎臟纖維化區域大小以及TGF-β, CTGF相關的纖維化路徑相較於對照組都有明顯的增加；而在經過跑步機運動訓練後相較於沒有運動訓練，血壓、纖維化區域的大小、TGF-β, CTGF相關的纖維化路徑皆有明顯的下降。藉由本實驗證明運動可以改善或是預防高血壓所導致的腎臟纖維化。因此本實驗發現在高血壓藉由運動訓練預防腎臟疾病提供了一個新的治療效果。

Introduction/Background. Several evidence indicates that fibrosis plays a critical role in the pathogenesis and progression of hypertensive kidney disease. Exercise training is well known to have various benefits for protection and treatment of hypertension-related kidney disease or kidney failure. However, the mechanisms of anti-fibrotic effects of exercise training on kidney remain unclear. Therefore, this study investigated the effect of exercise training on hypertension-induced renal damage and fibrosis. Material and Methods. Twenty male spontaneously hypertensive rats (SHR) were randomly divided into sedentary group (SHR, n=10) and hypertension rats underwent treadmill running exercise for 60 min / day, 5 sessions / week, for 12 weeks (SHR-EX, n=10). Age-matched ten male Wistar-Kyoto rats (WKY, n=10) were used as a normotensive reference group. After exercise training, the excised renal cortex from rats were measured by histopathological analysis and Western Blotting. Results The SHR group exhibited high blood pressure and interstitial fibrosis relative to WKY group. In addition, these alterations were accompanied by increases in renal cortex gene expression of transforming growth factor beta (TGF-β) and connective tissue growth factor (CTGF) involved in the fibrotic response. By contrast, this situation became better after exercise training interventions. Conclusion Our results indicated that exercise training could protecting against renal damage through improve of renal fibrosis-related pathway in hypertension. It suggested that exercise training would ameliorate of renal function in the population of hypertension.

中文摘要 I
Abstract II
致謝 IV
Context V
TABLE AND FIGURE CONTENTS VI
Introduction 1
Material and Methods 3
Animal model 3
Exercise training 3
Tissue Extraction 4
Western Blotting 4
Masson’s trichrome staining 5
Statistial Analysis 5
Result 6
Discussion 8
Table 11
Figures 12
References 21

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