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研究生:林維剛
研究生(外文):Lin, Wei-Kang
論文名稱:靈芝液態發酵液對於第 2 型糖尿病大鼠降血糖與降血脂效果之評估
論文名稱(外文):Evaluation of the hypoglycemic and hypolipidemic effects of submerged culture of Ganoderma lucidum on type 2 diabetic rats
指導教授:蔡國珍
指導教授(外文):Tsai, Guo-Jane
口試委員:周正俊顏聰榮吳彰哲
口試委員(外文):Chou, Cheng-ChunYan, Tsong-RongWu, Chang-Jer
口試日期:2015-06-25
學位類別:碩士
校院名稱:國立臺灣海洋大學
系所名稱:食品科學系
學門:農業科學學門
學類:食品科學類
論文種類:學術論文
論文出版年:2015
畢業學年度:103
語文別:中文
論文頁數:100
中文關鍵詞:靈芝液態發酵第 2 型糖尿病降血糖降血脂
外文關鍵詞:Ganoderma lucidumsubmerged culturetype 2 diabeteshypoglycemichypolipidemic
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頁次
壹、 前言 ……………………………………………………………………… 1
貳、 文獻整理 ………………………………………………………………… 2
一、 糖尿病 ……………………………………………………………………… 2
(一) 糖尿病的定義與診斷標準 ……………………………………………… 2
(二) 糖尿病的分類 …………………………………………………………… 3
(三) 糖尿病之併發症與治療 ………………………………………………… 4
(四) 第 2 型糖尿病動物誘導模式 ………………………………………… 5
二、 高脂飲食 ………………………………………………………………… 8
(一) 高脂飲食的定義 ………………………………………………………… 9
(二) 高膽固醇飲食對脂質代謝之影響 ……………………………………… 9
(三) 脂質代謝異常與其相關疾病 …………………………………………… 10
(四) 高脂飲食與脂肪組織 …………………………………………………… 11
(五) 脂肪組織中脂質代謝相關酵素 ………………………………………… 11
三、 靈芝 ……………………………………………………………………… 12
(一) 靈芝之栽培方式 ………………………………………………………… 13
(二) 靈芝之生理活性 ………………………………………………………… 14
四、 靈芝對糖尿病之影響 …………………………………………………… 17
參、實驗設計 ………………………………………………………………… 20
肆、實驗材料與方法 ………………………………………………………… 22
一、材料與設備 ……………………………………………………………… 22
(一) 材料 ……………………………………………………………………… 22
(二) 設備 ……………………………………………………………………… 24

頁次
二、實驗方法與步驟 ………………………………………………………… 25
(一) 菌種凍管保存與活化 …………………………………………………… 25
(二) 實驗樣品製備 …………………………………………………………… 26
(三) 動物飼養與分組 ………………………………………………………… 27
三、樣品分析 ………………………………………………………………… 28
(一) 血漿 ……………………………………………………………………… 28
(二) 肝臟及腎臟 ……………………………………………………………… 33
(三) 脂肪 ……………………………………………………………………… 40
(四) 小腸 ……………………………………………………………………… 42
(五) 糞便 ……………………………………………………………………… 43
(六) 攝食量、飲水量及排尿量 ……………………………………………… 44
四、統計分析 ………………………………………………………………… 44
伍、結果 ……………………………………………………………………… 45
一、靈芝培養液菌絲體及胞外多醣含量 …………………………………… 45
二、口服葡萄糖耐受性試驗和血脂測定 …………………………………… 45
三、大鼠體重、攝食、飲水、排尿、糞便及組織臟器重量變化 ………… 46
四、大鼠血漿生化指標 ……………………………………………………… 47
五、大鼠肝臟及腎臟生化指標 ……………………………………………… 49
六、大鼠脂肪組織荷爾蒙敏感酵素及脂蛋白脂解酶活 …………………… 51
七、大鼠小腸雙醣酶生化指標 ……………………………………………… 51
八、大鼠糞便脂質生化指標 ………………………………………………… 52

頁次
陸、討論 ……………………………………………………………………… 53
一、靈芝液態發酵液對第 2 型糖尿病大鼠降血糖之影響 ………………… 53
二、靈芝液態發酵液對第 2 型糖尿病大鼠降血脂之影響 ………………… 57
三、靈芝液態發酵液對第 2 型糖尿病大鼠肝腎功能之評估 ……………… 59
柒、結論 ……………………………………………………………………… 61
捌、參考文獻 ………………………………………………………………… 62

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