臺灣博碩士論文加值系統

皮膚老化是隨年齡變化由內生性或外生性體引起體內或外觀過程的的皮膚老化。皮膚健康和美容代表外觀的主要因素。隨著人類健康和清潔的意識，近期開發了各種抗衰老項目。天然蜂王漿 - 已被證明可以獲得許多保健的特性，如抗氧化、抗菌、抗腫瘤、抗發炎、抗過敏及抗衰老。蜂王漿已有研究證明出，透過促進膠原蛋白數量來減少或延緩皺紋和下垂，使皮膚看起來比真實皮膚年齡更年輕。在研究中，我們首次將蜂王漿和黃蓍膠結合在一起，透過超音波破碎機進行超音波乳化法製備微米顆粒。製備的微米膠囊呈球形，表面光滑，平均粒徑為1.37 ± 0.499 μm。為了評估新膠囊劑的功能，透過動物試驗測定了美白、TEWL 和膠原蛋白的生成。結果表明，RJMCs 有能力顯著改善美白、膠原蛋白功效及表皮水分流失（TEWL）

Skin aging is a skin deterioration with age caused by parallel endogenous or internal and exogenous or external processes. It was not by accident that skin health and beauty play a principal factor in representing long-range appearance. Along the consciousness of health and clean in human, various anti-aging projects have been developed recently. A natural ingredient – royal jelly – has been demonstrated to obtain many miraculous properties such as antioxidant, antibacterial, anti-tumor, anti-inflammatory, anti-allergic as well as anti-aging. Royal jelly has been shown in research to boost skin look younger than the real skin age by stimulating collagen amount which can reduce or delay wrinkle and sagging. In this study we combined royal jelly and trangacanth gum to create microparticles by a sonochemical microemulsion process which is carry out by ultrasonic homogenizer. The prepared microcapsules pointed out spherical shape, smooth surface and 1.37 ± 0.499 μm average size. In order to evaluate the functions of new capsule agent, we assayed the whitening, TEWL and collagen production via animal model. These results show that royal jelly microcapsules have power to improve significantly whitening, collagen effect as well transepidermal water loss (TEWL).

Chinese abstract ........................................................................................................... i
English abstract ......................................................................................................... iii
Acknowledgement ...................................................................................................... v
Table of Contents ...................................................................................................... vi
List of figures .......................................................................................................... viii
Abbreviations ............................................................................................................ ix
Chapter 1: Introduction .............................................................................................. 1
1.1 Overall view ................................................................................................... 1
1.2. Research objective ........................................................................................ 3
Chapter 2 - Literature Review .................................................................................... 4
2.1 Skin and Skin aging ....................................................................................... 4
2.2 Royal jelly -Chemistry and physicochemical properties ............................... 5
2.3. Emulsion and their role in microemulsion method ....................................... 8
2.4. Tragacanth gum and their property .......................................................... 10
Chapter 3: Material and methods ............................................................................. 12
3.1. Materials ................................................................................................... 12
3.2. Methods ....................................................................................................... 12
3.2.1. Preparation of microcapsules: .................................................................. 12
3.2.2. Morphology and size particles assay ....................................................... 13
3.2.3. Glycine Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) ...................................................................................................... 13
3.2.4. Effect of stirring on behavior release from royal jelly encapsulation ..... 14
3.2.5. Encapsulation efficiency .......................................................................... 14
3.2.6. Measurement of royal jelly microcapsules stability with different temperature and preserve time ........................................................................... 14
3.2.7. Determination of depigmenting activity, TEWL effect and collagen production promoting activity of RJMCs in vivo via mice ............................... 15
3.2.8. Statistical analysis .................................................................................... 16
3.2.9. Patents search ........................................................................................... 16
Chapter 4: Results .................................................................................................... 20
4.1. Effect of some parameter on the size of RJMCs ........................................ 20
4.2. Determination of royal jelly microcapsules stability in different conditions21
4.3. Evaluation of encapsulation efficiency ....................................................... 22
4.4. Determination of RJMCs release ability ..................................................... 22
4.5. Evaluation of TEWL effects of RJMCs via mice ....................................... 23
4.6. Measurement of whitening activity of RJMCs in mice .............................. 24
4.7. Determination of collagen production promoting activity of RJMCs in mice ............................................................................................................................ 24
Chapter 5: Discussion ............................................................................................... 26
Chapter 6: Conclusion .............................................................................................. 28
References ................................................................................................................ 29
Extended Abstract .................................................................................................... 45

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