臺灣博碩士論文加值系統

本研究以熱塑性聚胺基甲酸酯(thermoplastic polyurethane TPU)作為基材，加入聚乙二醇(PEG)當作造孔劑，以二甲基甲醯胺(DMF)和四氫呋喃(THF)當作溶劑做成中空管。然後利用紫外光臭氧機活化內表面，進行表面接枝聚合反應，使內表面產生羧酸基(-COOH)，進而將明膠(gelatin)固定於TPU表面，藉以增進中空管的親水性、生物相容性與血液相容性。所得TPU管以SEM觀察其表面型態、以XPS分析元素組成、以及拉伸試驗與接觸角、L929纖維母細胞探討細胞相容性與貼附性，並以APTT與PT分析血液相容性，找出最適合的參數，以作為人工血管之應用。結果顯示過氧化物量密度在臭氧處理30分鐘達最高值，材料並無釋放毒性物，凝血時間無異常，而以明膠作為延伸鏈，可大幅提高細胞貼貼附數量。

In this study, thermoplastic polyurethane (TPU) tubes were prepared through phase inversion method using a cosolvent of N,N-dimethyl formamide (DMF) and tetrahydrofuran (THF). The resultant tubes were subsequently treated with UV-ozone to activate the surface. After grafting with acrylic acid (AA), gelatin was covalently immobilized onto the surface.
Scanning electron microscope (SEM) was use to examine the surface morphology of the tube. Further characterization included XPS, tensile strength, burst pressure, contact angle, and hemocompatibility such as activated partial thromboplastin time (APTT) and prothrombin time (PT). In addition, in vitro cytocompatibility was evaluated with L929 fibroblasts. The results show that the modification did improve the adhesion of fibroblasts without cytotoxicity and thrombogenicity.

中文摘要 I
Abstract II
誌謝 III
圖索引 VIII
表索引 X
第一章 緒論 1
1-1 研究背景 1
1-2 研究目的 2
第二章 研究背景 3
2-1 生醫材料 3
2-1-1生醫材料簡介 3
2-1-2 組織工程 5
2-1-3 生物支架 6
2-1-4 血管介紹 7
2-1-5 人工血管當前發展 9
2-2 薄膜製備 10
2-2-1 薄膜製備的方法 10
2-2-2 造孔劑 11
2-2-3 造孔劑應用於薄膜 11
2-3 薄膜改質 12
2-4 血液凝固學原理 14
2-4-1 血液的組成 14
2-4-2 血液凝固理論 16
2-4-3 血液凝固因子 17
2-4-4 血液凝固程序 19
2-4-5 活化部分凝血活酶時間(activated partial thromboplastin time, APPT) 20
2-4-6 凝血酶原時間(Prothrombin time, PT) 21
2-5 實驗材料介紹 22
2-5-1 聚胺基甲酸酯(TPU) 22
2-5-2 TPU於薄膜應用 23
2-5-3 聚乙二醇(PEG) 24
2-5-4 明膠(gelatin) 24
第三章 實驗 25
3-1 實驗材料與藥品 25
3-2 實驗儀器 27
3-3 實驗流程 28
3-4 材料配製 29
3-4-1 以溶劑溶解熱塑性聚胺基甲酸酯 29
3-4-2 薄膜中空管製備 29
3-4-3 紫外光臭氧改質步驟 29
3-4-4 表面活化與接枝 30
3-5 材料物性測試 30
3-5-1 掃描式電子顯微鏡(SEM) 30
3-5-2 拉伸強力分析 30
3-5-3 環形拉伸測試 32
3-5-4 接觸角測定 34
3-5-5 XPS 35
3-6 血液相容性 36
3-6-1 活化部分凝血時間(Activated partial thromboplastin time, APTT) 36
3-6-2 凝血酶原時間(prothrombin time, PT) 37
3-6-3 蛋白質吸附 38
3-6-4 DPPH過氧化物分析 39
3-7 生物相容性 40
3-7-1 細胞株來源與培養條件 40
3-7-2 細胞株繼代 40
3-7-3 體外細胞毒性試驗(in vitro cytotoxicity) 41
3-7-4 MTT細胞存活率分析 42
3-7-5 細胞貼附 42
第四章 結果與討論 43
4-1 表面特性與機械性質 43
4-1-1 掃描式電子顯微鏡 43
4-1-2 拉伸強度試驗 46
4-1-3 環型拉伸強度試驗 48
4-1-4 DPPH分析 50
4-1-5 BCA分析 51
4-1-6 XPS 53
4-1-7 接觸角測試 55
4-2 血液相容性 57
4-3 生物相容性 59
4-3-1 體外細胞毒性測試 59
4-3-2 細胞貼附 64
第五章 結論與未來展望 67
5-1結論 67
5-2 未來展望 68
第六章 參考文獻 69

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