臺灣博碩士論文加值系統

肥胖會危害健康並且與代謝症候群表現的各種併發症息息相關，包括血糖、膽固醇、三酸甘油酯、血壓的上升，以及提高心腦血管疾病與診斷出第二型糖尿病的風險。而與其關聯的脂肪細胞，在調節脂質代謝和能量平衡中扮演了關鍵角色。文獻指出，紫番薯葉萃取物含有高量的多酚類，且被證實具有多方面的生物活性。本研究主要探討紫番薯葉萃取物 (PSPLE) 對3T3-L1前驅脂肪細胞分化的影響。利用MTT細胞存活試驗檢測，發現PSPLE可有效降低3T3-L1前驅脂肪細胞增生，但細胞死亡率並無改變，顯示PSPLE並不會造成細胞毒殺而抑制其增生。接著PSPLE前處理之3T3-L1前驅脂肪細胞，加入分化藥劑誘發細胞分化後，發現細胞內脂質的生成量減少。最後3T3-L1前驅脂肪細胞分化為成熟脂肪細胞後，處理PSPLE三天，發現PSPLE會使成熟脂肪細胞死亡；此外也會減少細胞內的脂質含量。本研究結果顯示紫番薯葉萃取物可以抑制3T3-L1前驅脂肪細胞增生及分化，並能抑制成熟3T3-L1脂肪細胞的脂滴積累，因此紫番薯葉可能具有潛力開發成為抑制肥胖的保健食品。

Obesity is a health hazard which is closely associated with metabolic syndromes which are manifested by many symptoms like elevated, glucose, cholesterol, triacylglycerol, increases in blood pressure, risk of cardiovascular disease, and heightened chances of being diagnosed as type II diabetes. Adipocytes play a critical role in regulating lipid metabolism and energy balance and are associated with adipose tissue mass and obesity. Purple sweet potato leaves (PSPL) contain a high content of anti-oxidants which have been shown to exhibit a number of biological activities. The aim of this study was to investigate whether purple sweet potato leaf extract (PSPLE) could affect differentiation of 3T3-L1 pre-adipocytes. Our data showed that PSPLE significantly decreased the proliferation of as determined by MTT assay, but didn’t lower cell viability by Trypan blue exclusion test. These results also indicated that no cytotoxicity on 3T3-L1 preadipocytes was observed after treatment with PSPLE. In addition, our results demonstrated that PSPLE induced anti-adipogenesis during the differentiation of 3T3-L1 preadipocytes. PSPLE may decrease the lipid content of differentiated 3T3-L1 cells via induction of cell apoptosis. Taken together, PSPLE may be beneficial to anti-obesity effect and preventing metabolic syndrome.

目錄.………………………………………………………………………………......Ⅰ
中文摘要……………………………………………………………………………..Ⅵ
Abstract ………………………………………………………………………………..Ⅶ
縮寫表……………………………………………………………………………......Ⅷ
致謝.…………………………………………………………………………….........Ⅸ
第一章 緒論..…………………………………………………………………………1
第一節 肥胖…………...…………………………………………………………...1
第二節 代謝症候群……………………………………………………….…….…3
第三節 脂肪組織……….…………………………………………………….........5
第四節 3T3-L1前驅脂肪細胞………………………………………….................8
第五節 紫番薯葉………………………………….………....................................11
第二章 研究動機與目的 ……………………………….…………….....………….13
第一節 研究緣起 ………………………………………………………………...13
第二節 研究目的 ………………………………………………………………...14
第三章 結果與討論 …………………………………………………………...........15
第一節 紫番薯葉水萃物清除自由基的能力 .......................................................15
第二節 紫番薯葉水萃物抑制3T3-L1前驅脂肪細胞的增生 …………………..17
第三節 紫番薯葉水萃物對3T3-L1前驅脂肪細胞脂質的生成…………………20
第四節 紫番薯葉水萃物對3T3-L1成熟脂肪細胞脂質的儲存與細胞存活率的影
響………………………………………………………………….………28
第五節 紫番薯葉水萃物對3T3-L1脂肪細胞中細胞激素分泌之影響..34
第六節 討論……………………………………………………………………….36
第四章 結論………………………………………………………………….………41
第五章 材料與方法.....................................................................................................42
第一節 研究材料...................................................................................................42
第二節 實驗方法...................................................................................................44
第六章 參考文獻與附錄.............................................................................................55
第一節 參考文獻.............................................................................................55
第二節 附錄.....................................................................................................59

Alam, T., An, M.R., and Papaconstantinou, J.(1992). Differential expression of three C/EBP isoforms in multiple tissues during the acute phase response. J Biol Chem 267, 5021-5024.

Asada, N., Takahashi, Y., and Honjo, M. (2000) Effects of 22K or 20K human growth hormone on lipolysis, leptin production in adipocytes in the presence and absence of human growth hormone binding protein. Horm Res 54, 203-207.

Ashammakh, N., Miettinen, S., Niemela, S., and Sarkanen J.R. (2008). Adipose Tissue and Adipocyte Differentiation: Molecular and Cellular Aspects and Tissue Engineering Applications. J Bio Tiss Eng 4, 3-14.

Bondet, V., Brand, W.W., and Berset C. (1997). Kinetics and mechanisms of antioxidant activity using the DPPH free radical method. Lebensm Wiss Technol 30, 609-615.

Cameron, A.J., Shaw, J.E., and Zimmet, P.Z. (2004). The metabolic syndrome: prevalence in worldwide populations. Endocrinol Metab Clin North Am 33, 351-375.

Chang, W.H., Hu, S.P., Huang, Y.F., Yeh, T.S., and Liu, J.F. (2010). Effect of purple sweet potato leaves consumption on exercise-induced oxidative stress and IL-6 and HSP72 levels. J Appl Physiol 109, 1710-1715.

Clarke, S.L., Robinson, C.E., and Gimble, J.M. (1997). CAAT/enhancer bindingproteins directly modulate transcription from the peroxisome proliferator-activated receptor gamma 2 promoter. Biochem Biophys Res Commun 240, 99-103.

Elberg, G., Gimble, J.M., and Tsai, S.Y. (2000). Modulation of the murine peroxisome proliferator-activated receptor gamma 2 promoter activity byCCAAT/enhancer-binding proteins. J Biol Chem 275, 27815-27822.

Farmer, S.R. (2006). Transcriptional control of adipocyte formtion. Cell Metab 4, 263-273.

Fasshauer, M., and Paschke, R. (2003) Regulation of adipocytokines and insulin resistance. Diabetologia 46, 1594-1603.

Gregoire, F.M., Smas, C.M., and Sul, H.S. (1998). Understanding adipocyte differentiation. Physiol Rev 78, 783-809.
Hayase, F., and Kato, H. (1984). Antioxidative components of sweet potatoes. J Nutr Sci Vitaminol 30, 37-46.

Haffner, S.M., Valdez, R.A., Hazuda, H.P., Mtchell, B.D., Morales, P.A., and Stern, M.P. (1992). Prospective analysis of the insulin-resistance syndrome (Syndrome X). Diabetes 41, 715-22.

Hotamisligil, G.S., Shargill, N.S., and Spiegelman, BM. (1993) Adipose expression of tumor necrosis factor-alpha: direct role in obesity-linked insulin resistance. Science 259, 87-91

Hou, W.C., Chen, Y.C., Chen, H.J., Lin, Y.H., Yang, L.L., and Lee, M.H. (2001). Antioxidant activities of trypsin inhibitor, a 33 KDa root storage protein of sweet potato (Ipomoea batatas (L.) Lam cv. Tainong 57). JAgric Food Chem 49, 2978-2981.

Hulver, M.W., Zheng, D., and Tanner, CJ. (2002) Adiponectin is not altered with exercise training despite enhanced insulin action. Am J Physiol Endocrinol Metab 283, 861-865.

James, M.N., and Kim Y.C. (2000). Adipocyte Differentiation and Gene Expression. J Nutr 130, 3122-3126

Justin, R.D., and Morris, J.B. (2009). Regulation of fat cell mass byinsulin in Drosophila melanogaste. Mol Cell Biol 29, 6341-6352.

Ka, S.O., Kim, K.A., Kwon, K.B., Park, J.W., and Park, B.H. (2009). Silibinin attenuates adipogenesis in 3T3-L1 preadipocytes through a potential upregulation of the insig pathway. Int J Mol Med 23, 633–637.


Kylin, E. (1923). Studien uber das Hypertonie-Hyperglyka ‘mie-Hyperurika’ miesyndrom. Zentralbl Inn Med 44, 105–127.

Lechner, S., Mitterberger, M. C., Mattesich, M., and Zwerschke, W. (2013). Role of C/EBPβ-LAP and C/EBPβ-LIP in early adipogenic differentiation ofhuman white adipose-derived progenitors and at later stages in immature adipocytes. Differentiation 85, 20-31.

Lehrke, M., and Lazar, M.A. (2005). The many faces of PPARγ. Cell 123, 993-999.

Ludvik, B.H., Mahdjoobian, K., Waldhaeusl, W., Hofer, A., Prager, R., Kautzky-Willer, A., and Pacini, G. (2002). The effect ofIpomoea batatas (Caiapo) on glucose metabolism and serum cholesterol in patients with type 2 diabetes: a randomized study. Diabetes Care 25, 239-240.

Macdougald, O.A., and Lane, M.D. (1995). Transcriptional regulation of gene-expression during adipocyte differentiation. Annu Rev Biochem 64, 345-373.

Navarrete, J.M.M., and Fernandez, J.M. (2012). Adipocyte Differentiation. Adipose Tissue Biology 2, 17-38.

Nguyen, L. Q., Nguyen, L. Q., Kopp, P., Martinson, F., Stanfield, K., Roth, S. I., and Jameson, J. L. (2000). A dominant negative CREB (cAMP response element-binding protein) isoform inhibits thyrocyte growth, thyroid-specific gene expression, differentiation, and function. Mol Endocrinol 14, 1448-1461.

Rajala, MW., Obici, S., Scherer, PE., and Rossetti, L. (2003). Adipose-derivedresistin and gut-derived resistin-like molecule-beta selectively impair insulin action on glucose production. J Clin Invest 111, 225-230

Rangwala, S.M., and Lazar, M.A.(2000). Transcriptional control ofadipogenesis. Annu Rev Nutr 20, 535-559.

Schadinger, S.E., Bucher, N.L., Schreiber, B.M., and Farmer, S.R.(2005). PPARγ2 regulates lipogenesis and lipid accumulation in steatotic hepatocytes. Am J Physiol Endocrinol Metab 288, 1195-1205.
Steppan, C.M., Bailey, S.T., and Bhat, S. (2001). The hormone resistin links obesity to diabetes. Nature 409, 307-312

Vague, J. (1947) Sexual differentiation. A determinant factor of the forms of obesity. Med Press 55, 339–340.

Yang, M. H., Vasquez, Y.,Ali, Z., Khan, I. A., and Khan, S. I. (2013). Constituents from Terminalia species increase PPARα and PPARγ levels and stimulate glucose uptake without enhancing adipocyte differentiation. J Ethnopharmacol 149, 490-498.
郭明勳 (2010)。紫番薯葉萃取物抑制脂肪細胞新生以及改善胰島素阻抗，中原大學生科技研究所碩士論文。
行政院衛生福利部國民健康局 (2013)。成人（20歲以上）代謝症候群判定標準對照表。
許雅婷 (2012)。紫番薯葉萃取物在脂肪細胞分化與代謝的影響。中國醫藥大學生科技學系碩士論文。