臺灣博碩士論文加值系統

本論文提出使用衰逝全反射架構之應用電光調變侷域性表面電漿共振進行生化檢測之系統，所使用的感測元件製作於具有良好電光效應的鈮酸鋰晶片上，其元件結構是由脊形結構、電極、與感測區所構成。在外加電壓下，可在鈮酸鋰材料內部產生電場，用以改變材料的折射率，進而調變表面電漿共振特性。感測區中所使用的表面電漿激發層為鍵結於金膜上奈米金粒所構成。感測區中的生化感測層是利用自組裝薄膜技術，將人血清球蛋白鍵結於表面電漿激發層上，可與治療冠狀心臟疾病之藥物－乙型阻斷劑產生鍵結，利用蛋白質與藥物的鍵結可即時檢測出乙型阻斷劑之濃度。量測系統架構包含光強度與相位移架構。系統操作上，在不同藥物濃度下，可量測電光調變表面電漿共振所產生的反射光強度與相位移角度隨外加電壓變化的關係，以檢測乙型阻斷劑濃度變化的情形。相較於傳統的強度檢測方式，所提出的生化感測器具有：準確性高、敏感度高、操作容易等優點。在未來應用上，所提出的生化檢測系統還可量測藥物分子與人血清球蛋白之交互作用之強度，以了解藥物在人體內作用的機制。

In this dissertation, we present an electro-optically modulated localized surface plasmon resonance biosensing system in the attenuated-total-reflection configuration, which can be used to measure the concentration of biochemical material. The sensing chip consists of a sensing region and one pair of electrodes on the ridge structure. When the voltage is applied on the electrodes, the electric field produced in lithium niobate electro-optically modulates the refraction index and changes the wave vector of the incidence lightwave. In this study, we use two kinds of measurement configurations, including optical intensity and phase shift configuration. Excited layer of surface plasmon formed by gold nanoparticle on gold film is adopted in the sensor. Human serum albumin (HSA) produced by the self-assembling method is used as the sensing layer to real-time sense the concentration of beta-blocker, which is a kind of medicine for heart disease. During the sensing measurement, the concentration of beta-blocker can be determined by the relation between the reflection intensity (or the phase shift) and the voltage. In comparison with the conventional SPR sensing, the proposed SPR sensing system has many advantages, such as: high accuracy, high sensitivity, and easy operation. In the future application, the presented biosensing system can be utilized to measure the interaction between HSA and medicines molecule for understanding the interaction mechanism of medicine in the human body.

中文摘要 i
英文摘要 ii
致謝 iii
目錄 iv
表目錄 vi
圖目錄 vii

第一章 緒論 1
1.1 前言 1
1.2文獻回顧 3
1.3研究動機 8
1.4章節內容 9

第二章 表面電漿共振理論與外差干涉儀原理 10
2.1 表面電漿共振簡介 10
2.2 表面電漿波之激發原理 12
2.3 光學激發表面電漿共振 20
2.4 外差干涉術簡介 24
2.5 鎖相放大器簡介 27
2.6 表面電漿共振量測方法與生化檢測原理 29

第三章 衰逝全反射架構之電光調變表面電漿共振檢測原理與檢測系統 31
3.1 鈮酸鋰材料特性 31
3.2電光調變表面電漿共振生化檢測技術之操作原理 39
3.3電光調變表面電漿共振生化感測器之元件設計 40
3.4生化檢測系統之架設 42

第四章 電光調變表面電漿共振生化感測元件之製作與量測 47
4.1 電光調變表面電漿共振生化感測元件之製程方法 47
4.2 電光調變表面電漿共振生化感測元件之製程步驟 51
4.3 奈米金粒之合成與選區固定 56
4.3.1奈米金粒合成 57
4.3.2奈米金粒選區固定 58
4.4 人血清球蛋白自組裝薄膜技術 63
4.5 乙型阻斷劑調配 66

第五章 結果與討論 67
5.1衰逝全反射架構 67
5.2電光調變表面電漿共振生化感測元件之光強度量測 69
5.2.1晶片脊形寬度為500μm、高度5μm之量測結果 69
5.2.2晶片脊形寬度為800μm、高度5μm之量測結果 71
5.2.3晶片脊形寬度為500μm、高度6μm之量測結果 73
5.2.4晶片脊形寬度為800μm、高度6μm之量測結果 75
5.2.5參數比較: 脊形寬度、脊形高度與入射光角度 77
5.3電光調變表面電漿共振生化感測元件之相位移量測 81
5.3.1晶片脊形寬度為500μm、高度5μm之量測結果 82
5.3.2晶片脊形寬度為800μm、高度5μm之量測結果 83
5.3.3晶片脊形寬度為500μm、高度6μm之量測結果 84
5.3.4晶片脊形寬度為800μm、高度6μm之量測結果 86
5.3.5參數比較: 脊形寬度、脊形高度與入射光角度 87
第六章 結論 92
參考文獻 94
中英文名詞對照表 98

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