臺灣博碩士論文加值系統

本光鑷夾相關研究依時序分為三階段，第一階段使用濕式化學蝕刻法研製光纖微透鏡，並使用Matlab影像處理功能進行光纖微透鏡之錐形角度測定。第二階段選用相同直徑與錐形角度但不同光纖蝕刻長度之圓錐形光纖微透鏡並分別建構光鑷夾系統，並使用650 nm雷射做為系統光源。第三階段分別對塑膠微粒、燕子牌即發酵母菌 (酵母菌A)及伯爵牌釀酒酵母(酵母菌B)進行光捕捉實驗，共分五項實驗。前四項實驗分別使用兩款相同直徑與錐形角度但不同光纖蝕刻長度之圓錐形光纖微透鏡建構出光鑷夾系統，並分別針對塑膠微粒與酵母菌A進行光捕捉實驗。藉由不同光纖蝕刻長度所架構之光鑷夾系統，探討其光纖蝕刻長度與捕捉效率之關係。第五項實驗使用此四項實驗中最高捕捉效率之光纖微透鏡建構出光鑷夾系統，並對酵母菌B進行捕捉實驗。從實驗結果得知，不同種類酵母菌具有不同逃脫速度及捕捉效率，藉此可鑑別兩廠牌酵母菌。
在光捕捉塑膠微粒之實驗中，使用蝕刻長度1.5 mm之圓錐形光纖微透鏡組成光鑷夾，光鑷夾之最佳捕捉效率為10.1%；然而，具蝕刻長度1.1 mm之系統之最佳捕捉效率為8.9%。在光捕捉酵母菌A之實驗中，使用蝕刻長度1.5 mm之圓錐形光纖微透鏡組成光鑷夾，光鑷夾之最佳捕捉效率為6.9%；然而，具蝕刻長度1.1 mm之系統之最佳捕捉效率為6.1%。最後在光捕捉酵母菌B的實驗中，使用蝕刻長度1.5 mm之系統之最佳捕捉效率為5.3%。在相同捕捉條件下捕捉酵母菌A與酵母菌B，其酵母菌A捕捉效率為6.9%；酵母菌B捕捉效率為5.3%。因兩種不同酵母菌之捕捉效率與對應之逃脫速度皆不相同，所以此方法可用來鑑別細菌種類之異同。

These are three phases in the study of optical tweezers. In the first phase, fiber microlenses are sequentially made by wet chemical etching. The resultant cone-shaped lenses are analyzed by a Matlab code with image processing ability to obtain the corresponding taper angles. In the second phase, construct several optical tweezer systems, each with a cone-shaped microlens. The microlenses used here are identical in diameter and taper angel but different in their etched fiber lengths. A 650-nm laser is used as the light source. In the third phase, there are totally five optical trapping experiments conducted individually and sequentially on polystyrene particles, instant yeasts (yeast A), and saccharomyces cerevisiae (yeasts B). The first four experiments have something to do with systems with various etched fiber lengths and two different samples: polystyrene particles and yeast A. The purpose of doing so is to study the relation between the etched firer lengths and the trapping efficiencies. The system with the best results is used to conduct the fifth and the last experiment to optically trap yeast B. The experimental results show that yeast A and yeast B are different in their escape velocities and trapping efficiencies. The results also provide a simple way to identify the brand name and type of yeasts.
In trapping polystyrene particles, the optical tweezer system constructed with single cone-shaped fiber microlenses with an etched fiber length of 1.5 mm is used and the corresponding optimal trapping efficiency is about 10.1%. On the other hand, the system with an etched fiber length of 1.1 mm obtains an optimal trapping efficiency of 8.9%. Likewise in trapping yeast A, the optical tweezer system constructed with a cone-shaped fiber microlens with an etched fiber length of 1.5 mm is used and the corresponding optimal trapping efficiency is about 6.9%. On the other hand, the system with an etched fiber length of 1.1 mm obtains an optimal trapping efficiency of 6.1%. The last in trapping yeast B, the optical tweezer system constructed with a cone-shaped fiber microlens with an etched fiber length of 1.5 mm is used and the corresponding optimal trapping efficiency is about 5.3%. Compared the results of trapping yeasts at the same conditions, the resultant trapping efficiencies for yeast A and yeast B are 6.9% and 5.3%；which provide an evidence and an optical mean that one can use to tell the difference between yeasts and bacteria of different types or brand names.

中文摘要 I
Abstract II
致謝 IV
目錄 V
圖目錄 VII
表目錄 XI
第1章 光鑷夾緒論 1
1.1 光鑷夾重要發展史 1
1.2 光鑷夾生醫應用 5
1.3 本實驗室光鑷夾研究 12
1.4 研究動機 28
1.5 論文架構 29
第2章 光鑷夾研究方法 30
2.1 研究架構 30
2.2 光捕捉原理 33
2.2.1 光纖微透鏡內部模態數 38
2.3 光纖微透鏡製程 39
2.3.1 濕式化學蝕刻法 40
2.3.2 圓錐形光纖微透鏡比較 41
2.4 光鑷夾硬體系統架構 44
2.5 光捕捉參數設定 53
2.5.1 工作距離計算 53
2.5.2 逃脫速度量測 53
第3章 研究結果與討論 56
3.1 光鑷夾之塑膠微粒捕捉(實驗一) 56
3.1.1 具蝕刻長度1.5 mm之圓錐形角光纖微透鏡 57
3.1.2 具蝕刻長度2.6 mm之圓錐形角光纖微透鏡 58
3.1.3 具蝕刻長度3.2 mm之圓錐形角光纖微透鏡 59
3.1.4 結果分析與比較 60
3.2 光鑷夾之塑膠微粒捕捉(實驗二) 61
3.2.1 具蝕刻長度1.1 mm之圓錐形角光纖微透鏡 61
3.2.2 具蝕刻長度2.1 mm之圓錐形角光纖微透鏡 62
3.2.3 具蝕刻長度3.0 mm之圓錐形角光纖微透鏡 63
3.2.4 結果分析與比較 63
3.3 光鑷夾之酵母菌A捕捉(實驗三) 66
3.3.1 具蝕刻長度1.5 mm之圓錐形角光纖微透鏡 67
3.3.2 具蝕刻長度2.6 mm之圓錐形角光纖微透鏡 68
3.3.3 具蝕刻長度3.2 mm之圓錐形角光纖微透鏡 69
3.3.4 結果分析與比較 71
3.4 光鑷夾之酵母菌A捕捉 (實驗四) 72
3.4.1 具蝕刻長度1.1 mm之圓錐形角光纖微透鏡 72
3.4.2 具蝕刻長度2.1 mm之圓錐形角光纖微透鏡 73
3.4.3 具蝕刻長度3.0 mm之圓錐形角光纖微透鏡 74
3.4.4 結果分析與比較 75
3.5 光鑷夾之酵母菌B捕捉 (實驗五) 77
3.5.1 具蝕刻長度1.5 mm之圓錐形角光纖微透鏡 78
3.5.2 結果分析與比較 79
第4章 結論與未來工作 82
參考文獻 84
簡歷表 87

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