臺灣博碩士論文加值系統

本文研究以雙光子聚合技術應用於大尺寸三維產品的設計與製造。本雙光子聚合製造系統使用130 kHz高重複率之Nd:YAG雷射、三維壓電平台，並搭配放大倍率50倍、數值孔徑0.8的物鏡製作細胞培養基質與疏水性結構。藉由直接聚焦雷射於樹脂而不通過玻片，再以蜂巢式製作方法避免結構倒塌，製作出具有曲面結構的細胞培養基質以及具有特定表面粗糙度的細胞培養基質，予以提供造骨細胞遷徙之相關研究。再者，本研究根據Cassie & Baxter模型，製作出不同設計參數的疏水結構並量測其接觸角，將其疏水結構製作於PDMS晶片之微流道中以進行二項流產生測試。比較兩者有無加入疏水性結構之流態分佈圖，可發現疏水性結構可有效減小環狀流之面積。最後，本研究建構了一組大範圍雙光子製造系統，其包含4瓦高功率飛秒雷射、振鏡掃描系統，壓電驅動平台以及傾斜校正平台。藉由整合以上元件，可製作邊長1毫米的方形光柵以及直徑3毫米的台灣大學校徽。

This thesis studies on design and fabrication of large scale 3D objects by two-photon polymerization. The established TPP fabrication system equipped with 130 kHz repetition rate Nd: YAG laser, 3D piezostage, and 50x microscope objective with NA=0.8 were used to fabricate substrates for cell migration and hydrophobic structures. With laser beam directly focused on resin without through coverslip and honeycomb bulkhead method for avoiding structures collapse, the substrates with curvature surface and the substrates with different surface roughness regions were fabricated and provided for studying the migrating behavior of human osteosarcoma cells. Moreover, according to the Cassie & Baxter model, hydrophobic structures with different design parameters were fabricated. The hydrophobic structure with droplets contact angle greater than 90 degree is made inside the channel of PDMS chip for emulsion generation. Comparing the flow regimes map of PDMS chip with and without hydrophobic structures, the annular flow region becomes narrower with hydrophobic structure. In addition, a large scale TPP fabrication system including 4W high power femtosecond laser, galvanometer scanner, piezo-driven translation stage and tilt stage was established. With the integration of these components, large-scale optical grating with size of 1mm x 1mm and the logo of National Taiwan University with 3mm diameter can be manufactured successfully.

口試委員審定書 i
致謝 ii
摘要 iii
Abstract iv
CONTENTS v
LIST OF FIGURES vii
LIST OF TABLE xii
NOMENCLATURES xiii
Chapter 1 Introduction 1
1.1 Background 1
1.2 Literature Review 3
1.3 Research Motivation 11
1.4 Thesis outline 12
Chapter 2 Principle and fabrication process of TPP 13
2.1 Fundamental principle of TPP process 13
2.2 The fabrication process of TPP 17
2.3 NTUMFS CAM system for TPP micro fabrication 18
2.4 Experimental setup of TPP micro fabrication based on 3D piezostage 19
2.5 Supercritical point drying process 21
2.6 Scanning electronic microscope image 24
Chapter 3 Large scale TPP system based on galvanometer scanner 25
3.1 Hardware development of large scale TPP system 25
3.2 Software design for large scale TPP system 30
3.3 2D & 3D fabrication with large scale TPP system 34
Chapter 4 Design and fabrication of substrates for cell migrating with TPP 40
4.1 Experimental setup for fabrication of substrates 40
4.2 Fabrication strategy of substrates with different curvature on surface 46
4.3 Fabrication of substrates with specific roughness 50
Chapter 5 Design and fabrication of hydrophobic structure with TPP 59
5.1 Design of hydrophobic structure 59
5.2 Fabrication and measurement of hydrophobic structure 61
5.3 Application of hydrophobic structure in PDMS chips for emulsion generation 66
Chapter 6 Conclusions and Suggestions 73
6.1 Conclusions 73
6.2 Suggestions 74
References 76
Appendix A: User manual of large scale TPP system 82
A.1 Program setting 82
A.2 Hardware Installation 87
Vitae 88

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