臺灣博碩士論文加值系統

本研究為了瞭解浮游生物群聚呼吸率(CR)與細菌呼吸率(BR)的調控機制，在一磷限制的亞熱帶水庫進行為期十年的採樣，頻度為每周∼每兩周一次（水溫低於10℃）。我們發現在夏季的時候群聚呼吸率和細菌呼吸率皆擁有較高的值與較大的變動。研究結果顯示，溫度與群聚呼吸率，細菌呼吸率，標準化群聚呼吸率(SCR)，標準化細菌呼吸率(SBR)皆有顯著正相關(p<0.05)。其和溫度的關係可用阿瑞尼斯方程式來表示：
R=R_o e^((-Ea)⁄kT)
這裡的Ea稱為活化能可以表示溫度與呼吸率的相關性，群聚呼吸率為0.80±0.04, 標準化群聚呼吸率為 0.34±0.06，細菌呼吸率為0.93±0.06，而標準化細菌呼吸率為0.39±0.06。這顯示了溫度也許是控制群聚呼吸率與細菌呼吸率主要因子。我們亦發現BR有較高的Ea 值，這暗示了在未來環境溫度改變的時候，細菌呼吸率會有較顯著的改變。
為了進一步探究溫度的影響，進行為期２年的溫度操控實驗。我們的結果顯示了，短期間的溫度改變下，群聚呼吸率活化能為0.69，細菌為1.07。而長期得觀測結果總和群聚呼吸率活化能為0.67，細菌為0.94。細菌對於環境溫度的敏感度要高於整個群聚。我們的結果也顯示了，溫度的改變會增加系統的不穩定。

With a ten-year weekly to biweekly data set taken from a P-limited subtropical reservoir, this study intends to explore the potential mechanisms in controlling the temporal variation of plankton community respiration rate (CR) and bacterial respiration rate (BR). Summer not only had high CR and BR value, but also high variation. The results showed that CR, BR, specific plankton community respiration (SCR), and specific bacterial respiration (SBR) all correlated with temperature significantly (p < 0.05). Activation energy, Ea, of CR is 0.80±0.04, SCR is 0.34±0.06, BR is 0.93±0.06, and SBR is 0.39±0.06. The results also indicated that temperature might be the major controlling factor for CR and BR. We also found that Ea-BR is high than Ea-CR, which indicate that BR was more sensitive to temperature than CR.
Temperature manipulation experiment provide a situation to estimate temperature effect in wilder range. However, the most common characteristic of temperature response, activation energy (Ea), might be more variation. Our result combined two years temperature manipulation, the result proved that BR is more sensitive than CR either short-term temperature change (CR:0.69 and BR: 1.07) or long-term seasonal variation(CR: 0.67 ± 0.05 and BR:0.94 ± 0.08). Temperature change would increase system unstability. Two years result comparison showed that environmental condition would affect result warming.

目錄Table of Contents
口試委員會審定書…………………………………………………………....…i
誌謝……………………………………………………………………………...ii
中文摘要………………………………………………………………………..iii
Abstract …………………………………………………………………….…...iv
List of Tables…………………………………………………………………...vii
List of Figures………………………………………………………………… viii
Chapter1 Introduction…………………………………………………………... 1
References…………………………………………………………………..8
Chapter2 Decadal variation of plankton community respiration………………..11
Abstract…………………………………………………………………....12
Introduction………………………………………………………………..13
Materials and methods…………………………………………………….15
Results…………………………………………………………………….18
Discussion and conclusion………………………………………………..20
References………………………………………………………………...22
Chapter3 Decadal variation of bacterial respiration……………… ……………34
Abstract……………………………………………………………………35
Introduction………………………………………………………………..36
Materials and methods……………………………………………………..37
Results……………………………………………………………………..40
Discussion and conclusion…………………………………………………41
References…………………………………………………………………42
Chapter4 Temperature manipulation of community respiration and bacterial respiration………………………………………………………….47
Abstract…………………………………………………………………...48
Introduction……………………………………………………………….49
Materials and methods……………………………………………………51
Results…………………………………………………………………….53
Discussion and conclusion………………………………………………..55
References…………………………………………………………………57
Chapter5 Conclusion……...………………………………………………….. .62
References………………………………………………………………... 64

List of Tables
Table 1. Average, standard deviations, maximums, and minimums of measured parameters in Fei-tsui reservoir………………………………………25
Table 2. Correlation matrix of all measured parameters……………………… 26
Table 3. Correlation matrix of measured parameters about BR……………......43
Table 4. Activation energy (Ea) of CR and BR in all treatments of temperature manipulation experiment……………………………………………...58
Table5. Ea of CR and BR for the literature and this study……………………..65


List of Figure
Figure 1. Study site, Fei-tsui reservoir, showing the dam site……………….…27
Figure.2 Decadal variation of measured parameters………………..……….…28
Figure 3. Box plot for monthly variation of CR…………………….……….…31
Figure 4. Relationship between of Temperature and CR…………….…………32
Figure 5. Relationship between of Temperature and SCR…………….………..33
Figure 6. Box plot for monthly variation of (A) BR and (B) BR/CR ratio…….44
Figure 7. Relationship between of Temperature and BR……………….………45
Figure 8. Relationship between of Temperature and SBR……………………..46
Figure 9. Demonstration of temperature change of Ea in short-time scale……..59
Figure 10. Temperature effect on CR and BR…………………………………..60
Figure 11.Box-plot for three treatments of CR and BR……………………..….61

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