臺灣博碩士論文加值系統

鎂合金質輕、彈性模數與人體骨骼相當接近，作為硬組織取代材料而植入人體後可自然降解以避免二次手術。然而鎂合金活性高，在水溶液環境下容易腐蝕，基於氟基磷灰石(FHA)具有良好生物相容性、生物活性及引導骨生能力，所以本研究藉由水熱法在175ºC、2小時、飽和蒸汽壓環境下，直接合成FHA鍍層並析鍍於AZ80鎂合金表面，目的為在水熱合成過程中藉由F─離子的部分取代，以獲得更佳之FHA鍍層特性，並藉由FHA鍍層改善AZ80鎂合金之抗腐蝕性與細胞反應性。研究結果顯示：在模擬體液環境下進行浸泡測試以及電化學腐蝕測試，浸泡32天後FHA鍍層依然具有保護鎂合金之特性，F離子取代使鍍層結構穩定。極化曲線結果顯示表面披覆FHA鍍層有效提高AZ80鎂合金抗腐蝕能力，相較於無摻雜F之HA鍍層以及AZ80鎂合金腐蝕電位顯著提高。體外細胞培養試驗後發現經過24小時之MG63骨細胞顯著增生並貼附於鍍層表面，顯示水熱法披覆FHA鍍層於AZ80鎂合金之生物反應性佳，為具有潛力的硬組織植入物材料。

Lightweight magnesium (Mg) alloys have a low elastic modulus, which value very close to the human bone. Biodegradable Mg alloys can be used for medical hard tissue implants in orthopedic applications without revision surgery. However, Mg alloys are notably characterized by low corrosion resistance in physiological solutions. On the basis of the excellent biocompatibility, bioactive and osteoconductive properties of fluorohydroxyapatite (FHA), a FHA surface coating was hydrothermally synthesized on the AZ80 Mg alloy at 175ºC under a saturated steam pressure atmosphere. The aim of this present study is not only to obtain an optimal FHA coating with the substitution of F─ ions, but improving corrosion resistances and cell responses of the AZ80 Mg alloy. Experimental results show that AZ80 Mg alloy can be protected by the FHA coating even though immersion in the simulated body fluid after 32 days. FHA coating stability is improved after the substitution of F─ ions. In addition, the corrosion resistance of AZ80 Mg alloy can be effectively improved by the deposition of FHA coating. In vitro cell culture results represent that significant proliferation and adhesion of MG63 cells on the FHA-coated AZ80 Mg alloy is demonstrated after testing for 24 hours. It is recognized that FHA-coated AZ80 Mg alloy shows a good biological reponses, and it can be considered as an attractive, excellent hard tissue substitute material for further biomedical applications.

中文摘要................................................ ..i
Abstract................................................iii
誌謝......................................................v
總目錄...................................................vi
表目錄....................................................x
圖目錄...................................................xi
第一章 緒 論.............................................1
第二章 文獻回顧...........................................3
2-1 生醫材料(Biomaterials)................................3
2-1-1 金屬材料............................................4
2-1-2 高分子材料..........................................4
2-1-3 陶瓷材料............................................5
2-2 生物可降解之鎂合金植入材...............................7
2-3 鎂合金表面改質處理...................................10
2-4 氫氧基/氟基磷灰石....................................13
2-5 水熱法(Hydrothermal synthesis)......................17
第三章 實驗方法及步驟....................................30
3-1 鎂合金基材前處理.....................................30
3-2 水熱法合成氟基磷灰石鍍層..............................31
3-3 低掠角X-ray之相組成分析(Grazing-Incidence X-Ray Diffraction, GI-XRD).....................................32
3-4 傅立葉轉換紅外線光譜分析(Fourier-Transform Infrared Spectrometer, FT-IR).....................................32
3-5 化學分析光電子光譜分析(Electron Spectroscopy for Chemical Analysis, ESCA).................................33
3-6 穿透式電子顯微鏡(Transmission Electron Microscope, TEM) .........................................................34
3-7 SEM微觀形貌組織及EDS成分元素分析......................35
3-8 表面親疏水性測試(Surface wettability)................35
3-9 表面粗糙度量測(Surface roughness)....................36
3-10 奈米壓痕試驗(Nanoindentation tests).................36
3-11 鍍層貼附力強度測試..................................37
3-12 FHA/AZ80抗腐蝕性測試................................37
3-12-1 模擬體液浸泡測試(Immersion tests) .................37
3-12-2 動電位極化曲線測試(Electrochemical tests) .........38
3-13 體外細胞測試(in vitro tests)........................39
3-13-1 細胞培養及細胞附著型態觀察(Cell culture and morphology)..............................................39
3-13-2 細胞增生活性分析(Cell proliferation)..............41
第四章 結果與討論........................................53
4-1 水熱合成FHAp粉末相分析與形貌觀察......................53
4-1-1 FHAp之相組成分析...................................53
4-1-2 FHAp之特徵官能基分析...............................54
4-1-3 FHAp之表面形貌觀察.................................55
4-2 水熱合成FHA鍍層特性分析..............................56
4-2-1 相組成分析.........................................57
4-2-2 特徵官能基分析.....................................59
4-2-3 化學鍵結能分析.....................................60
4-2-4 TEM顯微影像與選區繞射分析...........................62
4-2-5 水熱合成鍍層表面與縱剖面形貌........................64
4-2-6 鍍層潤濕性測試與接觸角量測..........................66
4-3 FHA鍍層機械性質探討..................................68
4-3-1 FHA鍍層表面粗糙度..................................68
4-3-2 奈米壓痕硬度測試...................................69
4-3-3 FHA鍍層附著性評估..................................71
4-4 FHA鍍層的耐腐蝕特性研究..............................72
4-4-1 模擬體液浸泡測試...................................72
4-4-2 動電位極化曲線測試.................................75
4-5 FHA鍍層之體外細胞試驗................................76
4-5-1 細胞型態...........................................76
4-5-2 細胞增生測試.......................................78
第五章 結 論...........................................111
參考文獻 ................................................113
Extended Abstract.......................................125
簡 歷 ................................................131

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