臺灣博碩士論文加值系統

本文研究薑黃素(curcumin)與不飽和鏈脂膜之作用，及光照對薑黃素吸附不飽和鏈脂膜的影響。利用isothermal titration calorimetry (ITC)測量薑黃素吸附不飽和鏈脂膜的自由能、焓及熵，以熱力學探討吸附過程。
實驗結果顯示薑黃素吸附不飽和鏈脂膜為放熱反應，且反應為自然發生，不需任何外力既可發生。此外，發現薑黃素經光照後水解的產物不僅無吸附熱產生，亦不影響薑黃素吸附脂膜的過程。

This thesis is to study the binding of curcumin to unsaturated lipid membrane, and the effect of light-inducing degradation on curcumin binding to membrane. We use isothermal titration calorimetry (ITC) to measure the binding free energy, the binding enthalpy and the binding entropy for understanding the thermodynamics of binding process.
The result reveals that, the curcumin binding to the unsaturated lipid membrane is an exothermic reaction, and without any external force , it reacts spontaneously. Moreover, we found that the curcumin''s result of hydrolysis has neither binding heat nor effect on the process of intact curcumin binding to membrane.

第一章 簡介
1-1 生物細胞膜 ••••••••••••••••••1
1-2 薑黃與薑黃素 •••••••••••••••••3
1-3 研究動機 •••••••••••••••••••5
第二章 實驗樣本備製
2-1 實驗樣本 •••••••••••••••••••7
2-2 curcumin 溶液製備 •••••••••••••••7
2-3 脂質微胞製備 •••••••••••••••••12
第三章 實驗
3-1 吸收度-測量curcumin濃度 •••••••••••15
3-2 Isothermal Titration Calorimetry 滴定實驗
-測量curcumin對脂膜吸附的能力 •••••••••••20
第四章 總結 ••••••••••••••••••••33
參考文獻 •••••••••••••••••••••34
附錄一 ••••••••••••••••••••••37
附錄二 ••••••••••••••••••••••38

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