臺灣博碩士論文加值系統

國內民眾健康意識提升，許多營養保健品越來越多元化，如何保護這些對人體具有營養保健之物質，免受外在環境破壞，將產品膠囊化便是其中方式之一。本研究使用疏水性之聚二甲基矽氧烷（polydimethylsiloxane, PDMS）搭配玻璃基板製作微流體通道，以2%褐藻酸鈉溶液作為包覆水相，含醋酸鈣的植物油溶液作為核芯油，來製備包覆牛樟芝萃取物之微膠囊。研究結果顯示，將PDMS通道表面修飾為親水性後，適當調整水相及油相之流速可穩定地形成膠囊。其中以2%褐藻酸鈉與含1.5%、2.0%、2.5%醋酸鈣之植物油溶液搭配所形成之微膠囊較不易破損，且2%褐藻酸鈉和2.5%醋酸鈣所製備之微膠囊最為穩定，其平均粒徑分別為149.40 ±1.67 μm及 172.75±1.75 μm。2%褐藻酸鈉與含2.0%、2.5%醋酸鈣及牛樟芝萃取物之植物油溶液所製備之微膠囊，經模擬胃液處理一小時後，其耐受（酸）性程度較高，分別具72.97%與78.20%之耐受（酸）性。未來可利用此方式，製備水包覆油（o/w）或油包覆水（w/o）之微粒，來包覆藥物或生物細胞等，期望在生醫及藥物釋放研究領域上有所應用與發展。

In this study, we described the process of formation of Taiwanofungus camphorates microcapsules by microfluidic system of polydimethylsiloxane (PDMS). To the side channels we administered the canola oil with addition of calcium acetate. The discontinuous phase was an aqueous solution of alginate. The result demonstrated that modified the surface chemistry of the channels to render it hydrophile which changing the rates of flow of the side channels and of the discontinuous phase that allowed to formation of Ca-alginate microbeads. To formulate alginate microbeads we used aqueous solution of 2.0%（w/w）alginate and a range of concentrations of calcium acetate in oil：between 0.5%, 1.0%, 1.5%, 2.0% and 2.5%（w/w）. The mean diameter was 149.40 μm and 172.75 μm of 2.0% and 2.5%（w/w）calcium acetate of Ca-alginate microbeads. And we found the microcapsules of 2.0% and 2.5%（w/w）calcium acetate of Ca-alginate microbeads in hydrochloric acid solution（pH 1.5）for one hour, the tolerance level was 72.97% and 78.20%. However, the formulation of Ca-alginate microcapsules, using a microfluidic chip was simple and fast. The use of microfluidics to control the delivery of ions of encapsulation of microbeads and for tuning the rate of release from Ca-alginate.

謝誌
中文摘要................................................I
英文摘要................................................II
總目錄..................................................III
表目錄..................................................VI
圖目錄..................................................VII
第一章 前言..............................................1
1.1 牛樟芝簡介.........................................1
1.1.1 牛樟芝成分.....................................2
1.1.2 牛樟芝生理活性..................................7
1.1.2.1 護肝作用...................................7
1.1.2.2 抗氧化能力.................................7
1.1.2.3 抗病毒能力.................................8
1.1.2.4 減輕酒精誘發之肝損傷.........................8
1.1.2.5 抑制腫瘤細胞生長之研究.......................8
1.1.2.6 毒性及安全性研究............................9
1.2 微膠囊............................................10
1.2.1 微膠囊及其特點.................................10
1.2.2 微膠囊的包覆壁材................................11
1.2.2.1 幾丁聚糖..................................11
1.2.2.2 阿拉伯膠..................................11
1.2.2.3 褐藻酸鈉..................................11
1.2.3 微膠囊的製備方式................................12
1.2.3.1 噴霧乾燥法.................................12
1.2.3.2 相分離法..................................13
1.2.3.3 離心擠壓製程...............................13
1.2.3.4 乳化硬化製程...............................14
1.2.3.5 平盤包覆..................................15
1.3 生物晶片..........................................16
1.3.1 微陣列晶片……….................................16
1.3.2 微流體晶片……….................................16
1.3.3 生物晶片的應用…................................17
1.4 研究動機與目的.....................................18
第二章 材料與方法........................................19
2.1 實驗架構與設計.....................................19
2.2 實驗材料..........................................20
2.2.1 藥品.........................................20
2.2.2 儀器設備......................................20
2.3 微流體通道製作.....................................22
2.3.1 光罩設計與製作.................................22
2.3.2 玻片清洗......................................23
2.3.3 微影製程......................................23
2.3.3.1 負光阻塗佈................................23
2.3.3.2 軟烤.....................................25
2.3.3.3 曝光.....................................25
2.3.3.4 曝後烤...................................25
2.3.3.5 顯影.....................................26
2.3.3.6 硬烤.....................................26
2.3.4 PDMS翻模.....................................26
2.3.5 剝模.........................................27
2.3.6 PDMS表面修飾..................................27
2.3.7 氧電漿黏合....................................27
2.4 微膠囊製備........................................28
2.4.1 牛樟芝萃取液製備...............................28
2.4.2 褐藻酸鈉溶液配製...............................28
2.4.3 醋酸鈣植物油溶液配製............................29
2.4.4 牛樟芝萃取液膠囊化..............................29
2.5 微膠囊分析........................................30
2.5.1 粒徑大小及粒徑分佈.............................30
2.5.2 耐受（酸）性測試...............................30
2.5.3 釋放之測試....................................31
2.5.4 粗三帖含量測定.................................31
第三章 結果與討論........................................32
3.1 PDMS微流體通道實際尺寸.............................32
3.2 利用微流體通道製備膠囊..............................33
3.3 不同醋酸鈣濃度製備微膠囊之情形.......................38
3.4 以含不同濃度醋酸鈣的植物油與2%褐藻酸鈉溶液在微流體系
統中形成的微膠囊粒徑大小及分佈........................42
3.5 耐受（酸）性測試..................................56
3.6 釋放率測試.......................................56
第四章 結論與展望.......................................61
參考文獻...............................................62

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