臺灣博碩士論文加值系統

針對牙周病與蛀牙問題，患者容易有齒槽骨萎縮之症狀伴隨出血等情況。本研究開發一種治療牙齒脫落後造成的齒槽骨缺損之填充材料應用於齒槽骨保存手術。本實驗設計此填充材料含有三種成分：壓電陶瓷鈦酸鋇（BT）、β-三鈣磷酸鹽（β-TCP）和膠原蛋白。 BT具有人骨的壓電特性，可自發極化產生電訊號刺激骨生長，β-TCP具有良好的生物降解性，膠原蛋白能起到凝血作用，有助於術後止血及修復，並透過冷凍乾燥法在填充材上建立孔洞，提供組織和血管的生長空間。基於上述優點，填充材預計可提高患者齒槽骨的恢復速度，縮短療程，從而減輕患者的痛苦。此研究中製備了不同比例之BT/β-TCP結合膠原蛋白的多孔複合材料。而後通過X射線繞射（XRD）分析晶體結構，利用掃描電子顯微鏡研究微結構和形貌；藉由壓汞測孔儀測量孔隙率及孔洞大小；萬能試驗機測量其機械性質；毛細作用測試其對液體吸附速率。生物相容性測試則使用ISO10993-5之濃度標準進行細胞存活率分析(MTT assay)。結果顯示噴霧乾燥法造粒之45wt%BT/ 45wt%ꞵ-TCP/ 10wt%collagen複合材料有較佳的性質穩定性，其孔隙率為85.25%，機械強度3.19±0.41 MPa，28天之生物降解量為8.69±0.40wt%，生物相容性之細胞存活率達99.86±2.17%，並且在毛細作用測試有相對快速的液體吸附速率表現，有助於臨床應用上快速吸附並穩定血塊的效果。

Periodontitis and cavities would cause tooth exfoliation and bone defects with bleeding. To solve this problem, alveolar ridge preservation is needed. This study aims to develop a dental implant to treat bone defects of shed teeth in alveolar ridge preserving surgery. This dental implant contains three components, barium titanate (BT), β-tricalcium phosphate (β-TCP), and collagen. BT shows piezoelectric properties, which can produce an electric charge that promotes bone repair and reconstruction conducive to bone healing. β-TCP provides good biocompatibility. Collagen helps blood clot well, and porosity of filling material created by the freeze-drying method provides a three-dimensional space for tissue and blood vessel growth. Based on these three properties, the dental implant accelerates the recovery of patients’ tooth alveolar bones in order to alleviate the pains of patients. In this study, BT/β-TCP/Collagen-contained fillers were prepared. Several characterization techniques were carried out to analyze the properties of these materials. Initially, the crystal structures were analyzed by X-ray diffraction (XRD), while the microstructures and morphologies were investigated by scanning electron microscopy. The porosity and pore size are measured by a mercury porosity meter and the mechanical properties of compressive strength and biodegradability are tested. Absorption rate is carried out by capillarity test. Biocompatibility test were conducted in accordance with the ISO 10993-5 standard. The 45wt%BT/ 45wt%ꞵ-TCP/ 10wt% collagen specimen exhibits significantly improved the porosity (85.25%), compressive strength (3.19±0.41 MPa), biodegradability (8.69±0.40wt%), and cell viability 99.86±2.17%.

摘要 I
Abstract II
目錄 VI
圖目錄 X
表目錄 XVI
第一章 前言 1
1.1研究背景 1
1.2研究動機 2
第二章 文獻回顧 3
2.1 齒槽骨 3
2.1.1齒槽骨缺損之原因 5
2.1.2齒槽骨缺損之治療 7
2.2 壓電效應 9
2.2.1骨的壓電效應 11
2.3 壓電材料 12
2.3.1 壓電材料種類 12
2.3.2 鈦酸鋇之組成及性質 13
2.4 生醫陶瓷 14
2.4.1 生醫陶瓷材料種類 15
2.4.2 三鈣磷酸鹽之組成及性質 17
2.5 膠原蛋白 19
2.5.1 膠原蛋白種類 19
2.5.2 膠原蛋白之性質 20
2.6 壓電生醫陶瓷複合材料 21
2.6.1 壓電生醫陶瓷複合多孔支架之條件 23
第三章 實驗方法與目的 24
3.1 實驗設計 24
3.2 實驗藥品 27
3.3實驗儀器設備 29
3.4 樣品製備方法 31
3.4.1 β-三鈣磷酸鹽粉末 31
3.4.2 鈦酸鋇/β-三鈣磷酸鹽/膠原蛋白複合填料 33
3.5 樣品性質之分析方法 35
3.5.1 X光繞射分析儀 35
3.5.2 場發射掃描式電子顯微鏡 35
3.5.3 壓汞測孔儀 37
3.5.4 萬能試驗機 38
3.5.5 體外生物降解行為 38
3.5.6 體外生物相容性評估 39
3.5.7 毛細作用測試 40
第四章 實驗結果 41
4.1陶瓷粉末性質分析 41
4.1.1晶相分析 41
4.1.2陶瓷粉末形貌及粒徑分析 43
4.2 BT/ꞵ-TCP/collagen之複合支架分析 45
4.2.1晶相分析 45
4.2.2巨觀形貌 45
4.2.3微結構分析 46
4.2.4孔洞分析 48
4.2.5壓縮試驗 50
4.2.6體外生物降解行為 51
4.2.7體外細胞活性評估 52
4.2.8毛細作用測試 53
4.3 SD granulated- BT/ꞵ-TCP粉末 55
4.3.1晶相分析 55
4.3.2粉末形貌及粒徑分析 55
4.4 SD granulated- BT/ꞵ-TCP/collagen之複合支架分析 58
4.4.1晶相分析 58
4.4.2巨觀形貌 58
4.4.3微結構分析 60
4.4.4孔洞分析 61
4.4.5壓縮試驗 63
4.4.6體外生物降解行為 64
4.4.7體外細胞活性評估 65
4.4.8毛細作用測試 66
第五章 結果討論 68
5.1 BT/ꞵ-TCP/collagen複合支架之微結構與降解行為探討 68
5.2 BT/ꞵ-TCP/collagen複合支架之粉末團聚與分散性對抗壓強度之影響 72
5.3 BT/ꞵ-TCP/collagen複合支架孔洞分布和毛細作用的關聯性探討 75
5.4 BT/ꞵ-TCP/collagen複合支架綜合評估 76
第六章 結論 77
第七章 未來工作 78
參考文獻 79

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