本研究所採用之同位酵素變異方法，主係根據電泳分離法之原理，乃目前研究遺
傳變異之新穎而精確之方法。
　　本實驗之程序包括：(1)試劑之配製；(2)水溶性蛋白質之抽取；(3)電泳分離；及(4)
染色等四大步驟，每一樣本重複3次。
　　自全省不同之地區採集台灣杉種子苗、幼樹、成熟樹及嫁接幼樹之針葉，藉分析
其過氧化同位酵素之電泳型式，以研究：(1)台灣杉過氧化同位酵素之個體變異；(2)不
同樹齡台灣杉過氧化同位酵素之變異；(3)台灣杉過氧化同位酵素之季節變異；(4)不同
地區台灣杉過氧化同位酵素之變異；及(5)來自不同地區母樹之嫁接幼樹過氧化同位酵
素之變異。
　　根據分析之結果，發現各來源台灣杉過氧化同位酵素之電泳型式未呈顯著之差異。

　　The approach of this study on isoenzyme variation which emploies the principle of electrophoresis is a new and pricise means for the study of genetic variation at present.
　　The experimental procedures were essentially similar to those described by Miyazaki et al.(1969) and Chu et al. (1972). They contained (1)preparation for reagents and samples (2)extraction of soluble protein (3)electrophoretic separation, and (4)staining. Three replications were made for each of the sample.
　　Samples of needle-leaves of Taiwania crypto-merioides Hay. Were collected from various locations of Taiwan, then analyzed for the isoenzyme variation patterns of peroxidase. The results showed that: (1)needle-leaves collected from various parts within the same tree exhibited the same pattern of peroxidase isoenzymes; (2)peroxidase isoenzymes exhibited different patterns in various ages, but all combined with five bands; (3)the activity of peroxidase isoenzymes varied in different seasens and showed the lowest level in rich year. (4)the patterns of peroxidase isoenzymes of both grafted saplings and their mother trees were identical.
　　According to the results of the analysis of the peroxidase isoenzymes of Taiwania cryptomerioides Hay., no significantly genetic variations were found among different sources of Taiwania cryptrmerioides Hay.