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研究生:李雅琴
研究生(外文):Lee, Yah-Ching
論文名稱:植物荷爾蒙對水稻種子及幼苗arylacylamidase活性的影響
論文名稱(外文):Effects of Plant Hormones on the Activity of Aryl Aylamidase in Rise Seeds and Seedlinhs
指導教授:蔡文福蔡文福引用關係
指導教授(外文):Tsai, Wen-Fu
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:農藝學研究所
學門:農業科學學門
學類:一般農業學類
論文種類:學術論文
論文出版年:1977
畢業學年度:65
語文別:中文
論文頁數:50
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1.在in witro的情形下,各種參試荷爾蒙本身對 aryl acylamidase
的活性沒有顯著影響,既不會增加亦不會降低酵素活性。
2.不論在半粒種子或全粒棧子,aryl acylamidase 的活性均隨浸種
日數的增加而逐日下降。不過在無胚半粒種子的下降速度大於含胚
半粒種子及全粒種子。
3.GA3處理具有促進無胚半粒種子 aryl acylamidase 活性降低的
效果,且隨GA3濃度提高而增加,至1O(-5)M時達最大;ABA單
獨處理時無效果,但卻能抑制GA3的作用;IAA,2,4—D及
kinetin各處理對酵素的活性則無顯著影響。
4.荷爾蒙處理對全粒種子 aryl acylamidase 活性的影響除IAA可
略增加活性外,其他荷爾蒙的效果皆與上項無胚半粒種子相似,不
適在各種荷爾蒙處理效果間的差異不似無胚半粒種子大。
5.硫酸鈣亦能促進無胚半粒種子 aryl acylamidase 活性的下降,尤
其與GA3共同處理時更為明顯。
6.Cycloheximide 處理具有防止種子或葉片段 aryl acylamidase
活性的下降﹔亦能消除荷爾蒙的處理效果。
7.Cycloheximide處理的時間,以水為浸種培養液時,僅在浸種培養
兩天內加入效果較大,超過兩天後再加入,效果即不顯著﹔以GA3
為浸種培養液時,任何一天加入皆有效果。
8.將分離的葉片段浸種培養于荷爾蒙的結果,GA3仍能促進酵素活
性的降低﹔kinetin 及IAA稍能提高酵素活性﹔ABA及2,4-D處
理者與對照組相仿,並且與未經浸種培養的新鮮葉片段活性相當。
9.光線似可消除部分荷爾蒙在分離藥片段的處理效果,但對酵素的
turnover速率無影響。
10.發芽一星期的水稻幼苗在水耕液中加入各種荷爾蒙的處理結果。
GA3及IAA對葉汁酵素活性均無影響﹔2,4-D處理兩星期後酵
素活性略增,但兩星期後,則效果消失﹔kinetin 處理兩星期後酵素活性大增。
11.本試驗結果顯示,水稻種子浸種培養期間,aryl acylamidase
似無重新合成的現象發生,而其活性的降低,可能是浸種培養期間
,種子內蛋白質分解酵素活性增加使此酵素受到分解的結果,此可
由GA3及cycloheximide的處理效果而獲得間接證明。
The purpose of this study was to find out the effects of plant hormones on the activity of aryl acylamidase during rice germination and seedling growth. Rice seeds, leaf segments and seedlings were treated or incubated with GA3, ABA, IAA, 2,4-D, and Kinetin respectively for several days or weeks at controlled temperature. They were then assayed for the aryl acylamidase activity. The results were summarized as follows:
1.Incubation of the hormones in the assay mixtures with partially purified aryl acylamdiase indicated that hormones did not affect enzyme activity in vitro.
2.A decrease in the activity of aryl acylamidase In both incubated half seeds and germinating intact seeds was observed. The decrease was more rapidly in embryoless half seeds than embryo half seeds or intact seeds during incubation.
3.GA3 treatment could effectively promote the decreasing rate of enzyme activity in embryoless half seeds. The effect was increased with increasing concentration and reached a maximum at 10(-5)M. ABA alone was no effect, but it could overcome the effect of GA3. IAA, 2,4-D and kinetin had no significant effects on enzyme activity.
4.The effects of hormone treatment on intact seeds was the same as in embryoless half seed except of IAA which could slightly increase enzyme activity, but the effects were smaller than the embryoless half seeds.
5.CaSO4 could increase the decreasing rate of enzyme activity in embryoless half seeds especially when treated with GA3.
6.When cycloheximide was added into the incubation medium, the activity of aryl acylamidase in the seeds and leaf segments could be conserved. The antibiotic also completely eliminated the effects of hormone treatment.
7.A process sensitive to inhibit the decrease of enzyme activity by cyclohexmide occurred between 0 and 2 days after incubation when the embryoless half seeds were incubated in water, but the time of sensitivity to cycloheximide always existed when the seeds were incubated in GA3.
8.Leaf segments incubated with hormones for 3 days showed that GA3 still increased the decreasing rate of enzyme activity; kinetin and IAA slightly Increased the enzyme activity; ABA and 2,4-D were no effects.
9.Treatment of hormones in the nutrient solution showed that GA3 and IAA could not influence enzyme activity; after 2 weeks of treatment, kinetin could largely increase enzyme activity; after 1 week of treatments, 2,4-D could slightly increase enzyme activity, but was disappeared after 2 weeks of treatment.
10.Results of the present study suggested that de novo synthesis of aryl acylamidase appeared to be not occurred in rice embryoless half seeds during incubation. The enzyme probably was synthesized before seed maturation and gradually degraded by proteolytic enzymes during germination.
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