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研究生:蔡百豐
研究生(外文):Tsai, Pai-Feng
論文名稱:應用Sep-pakC18Cartridges來快速及有效的萃取人蔘的有效成分
論文名稱(外文):Rapid and Convenient Extraction of Ginsenosides from Panax Ginseng and Ginseng Drug Preparations Using Sep-pak C18 Cartridges
指導教授:薛文發薛文發引用關係
指導教授(外文):Sye, Wen-Fa
學位類別:碩士
校院名稱:淡江大學
系所名稱:化學學系
學門:自然科學學門
學類:化學學類
論文種類:學術論文
畢業學年度:74
語文別:中文
論文頁數:51
中文關鍵詞:人蔘萃取
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  從人蔘根,人蔘葉中抽取人蔘皂□一般常用的方法,包括以溶劑或吸附劑萃取均避免不了液相平衡需時甚久與步驟繁雜的缺點。本文中利用Sep-pak C18 cartridges於數分鐘內可將人蔘根,人蔘葉和GINSANA-GI15膠囊中的人蔘皂□成分抽取出來,這提供了一種簡便且快速萃取人蔘有效成分的方法。
  經Sep-pak萃取後的人蔘皂□並應用高效率薄層層析法(High Performance Thin-Layer Chromatography)及高效率液相層析儀(High Performance Liquid Chromatography)來分析。高效率薄層層析法中所使用的Kieselgel 60 F254 HPTLC plates(10×10cm)由Merck公司購得,展開液為氯仿-甲醇-水(65:35:10,下層液)。展開後的薄片以10%硫酸溶液噴灑並在110℃下加熱10分鐘以顯色。高效率液相層析儀分離人蔘皂□Rb1,Rb2,Rc,Rd的實驗部份,使用□Bondapack分離管柱時,流動相為氰甲烷:水(29:71),流速2ml/min;或使用TSKgel ODS-120T分離管柱分離,則流動相為氰甲烷:水(27:73),流速1.2ml/min。以上兩分離狀況均以紫外光偵測器偵測,波長為203nm。


  Common preliminary procedures for the isolation of ginsenosides (triterpene saponins) from ginseng roots and leaves involve solvent extraction or extraction by adsorbents. Such methods have been time consuming and the use of multiple step procedures. In the present study, a simple and reproducible method was developed using a Sep-pak C18 cartridge as a clean-up step for the analysis of ginsenosides in ginseng roots, leaves and GINSANA -GI15 capsules. Relatively large volumes of water extract can be concentrated to 1.5 ml (in methanol) in a few minutes, and an aliquot of water-methanol extract by different cartridges results in ginsenoside profile analyses which are identical.
  The methanol extracts from the Sep-pak extraction were analyzed by high performance thin layer chromatography (HPTLC) and reverse phase high performance liquid chromatography (HPLC). Chromatography with HPTLC was done on Merck precoated kieselgel 60F254 plates (10 ×10 cm). As a developing solvent, CHCI3-MeOH-H2O (65:35:10, lower layer) mixture was used. The spots were detected by spraying 10% sulfuric acid followed by heating. For HPLC determinations, the separation is achieved under the following conditions: the mobile phase was 29% acetonitrile in water at a flow rate of 2 ml/min on □Bondapack C18 column or the mobile phase was 27% acetonitrile in water at a flow rate of 1.2 ml/min on TSKgel ODS-120T column. The ginsenosides are directly detected at 203 nm without derivatization.

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