臺灣博碩士論文加值系統

　　蓮子（Nelumbo nucifera Gaertn）子葉所含的儲存蛋白質，依其在不同溶劑的可溶性分別加以萃取與定量。其中以蛋白素（albumin）的含量最多，約佔百分之六十二。其次是榖蛋白（glutelin）約佔百分之二十六。子葉所含的蛋白素呈複雜的異質性，經SDS-電泳分析後，大致可區分為七群多胜□類，其中99kd及54-59kd兩多胜□群，經過硫酸銨的分割及二次Sephadex G-100分子篩的分離而得以純化，對於99kd多胜鏈的胺基酸成分分析，發現含有較高的麩胺酸／麩胺酸醯胺（Glu/Gln）、甘胺酸（Gly）及息寧胺酸（Thr），而天門冬胺酸／天門冬醯胺（Asp/Asn）、異白胺酸（Ile）及酥胺酸（Tyr）則含量很低。
　　種子發育期間，子葉中多胜類的含量隨著種子的成熟而增高，其中以第七發育期中多胜類在質與量上皆有急劇的改變，顯示在此發育期是儲存蛋白合成最旺盛的階段。
　　蓮子萌發初期，子葉中儲存蛋白的消耗並不明顯，一直到第十天以後，儲存蛋白含量才急速減低。由SDS-電泳分析的結果顯示第一至第五群多胜類的含量，同時被分解而逐漸消失，可見此時在子葉內蛋白分解?的活性相當活躍。

　　The storage protein in the cotyledon of lotus (Nelumbo nucifera Gaertn) has been isolated by sequen-tial extraction according to the solubility of the proteins. Albumin fraction was found to be the major storage protein which constituted approximately 62% of the total protein. The glutelin fraction which was solubilized under highly denaturing condition constituted approximately 2 6% of the total protein.
　　The polypeptides of albumin fraction observed by SDS-PAGE electrophoresis were highly heterogeneous and composed at least seven polypeptide groups. Two polypeptide groups, 99kd and 54-59kd, were purified by ammonium sulfate fractionation and two cycles of Sephadex G-100 gel filtration. Amino acid composition of the 99kd polypeptide has been analyzed. It conteined more glutamic acid/glutamine, glycine and threonine but less aspartic acid/asparagine, isoleucine and tyrosine in compared to other proteins.
　　Accumulation of storage proteins in the cotyledon of developing seed was analyzed. The polypeptide groups increased gradully during early development.while a dramatic change in quantity as well as quality of teh polypeptides were observed in the seventh developing stage. the result indicates that the major storage protein is most activity synthesized during this transit period between sixth and seventh developing stages.
　　Storage protein in the lotus cotyledon was obviously decreased ten days after germination, which might be corresponding with the higher activity of the protease.