臺灣博碩士論文加值系統

碳穩定同位素比值一直普遍地應用在煤、原油及油母質的分析工作上，諸
如：以甲烷的碳穩定同位素比值來決定油母質的成熟度；由甲烷的碳穩定
同位素比值和其他高級烷類的碳穩定同位素比值之對比來推論有機物質的
成熟度；比較氣體碳氫化合物與煤的碳穩定同位素比值來推論有機物質的
來源；由沈積岩的有機物質碳穩定同位素比值以推論沈積環境。文獻上所
有關於碳穩定同位素比值的分析工作尚局限於不同分子間的碳穩定同位素
比值之比對及探討，而尚未發現有關於探討分子內碳穩定同位素比值分佈
情形的報告，故本論文的目的即是想利用甲烷單氧化■將丙烷與丁烷氧化
成2-丙醇與2-丁醇後，它們具有不等位碳的特性，然後分別去偵測每個碳
的碳穩定同位素比值，以比較分子內碳穩定同位素比值之分佈情形。目前
由實驗的結果已然發現：2-丙醇( 或丙酮 )上的第一個碳與第二個碳兩者
的碳穩定同位素比值有明顯的不同。倘若將來能順利地完成丙烷和丁烷的
分析，預期將可得到其上第一個碳會較第二個碳有較小的碳穩定同位素比
值，進而間接證明 Chung等人在西元1988年所提出的假設是否為正確，即
天然氣中甲烷含較小碳穩定同位素比值的現象乃是" 動力同位素效應 "控
制的結果。由此分子內不等位碳的同位素比值，或許可追溯其生物來源和
更進一步應用於油氣的對比分析上。

Carbon isotope ratio analyses for propane and butane have
been reported for years and become part of routine tools for
geo -chemical studies. Recently, Chung et al. proposed an ideal
equation to explain the kinetic isotope effects of petroleum-
forming process.However so much assumption had made before
deriving this ideal equation. The aims of this study is to
search the directly evidence to support this fact . Thus ,
propane and butane were oxidized by methane monooxygenase
catalyzed reaction to the corresponding 2- propanol and
2-butanol . The 2-propanol will be conducted to the iodoform
reaction to yield ethanol . The intramolecular C-13 isotope
ratio can be calculated from assay of ethanol and 2-
propanol. Similarly,the butane molecule were analyzed. Because
short of methane monooxygenase , meanwhile the most works were
focused on these 2-propanols and acetone obtained from
industrial company.The result indicated that the 2-propanol
and acetone from different sources might have different
C-13 stable isotope ratio. For those with similar bulk data
molecules can be further identified by comparing their
intramolecular isotope ratio of corresponding carbons.