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研究生:劉炳陽
論文名稱:豬丹毒桿菌分離株血清型鑑定、病原性狀與藥物感受性試驗
論文名稱(外文):Studies on Serotyping, pathogenicity and drug sensitivity test of Erysipelothrix rhusiopathiae isolates
指導教授:劉正義劉正義引用關係
學位類別:碩士
校院名稱:國立中興大學
系所名稱:獸醫研究所
學門:獸醫學門
學類:獸醫學類
論文種類:學術論文
論文出版年:1993
畢業學年度:81
語文別:中文
論文頁數:84
中文關鍵詞:豬丹毒桿菌血清型鑑定
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  自1992年3月至1993年2月份由養豬埸糞材,悄屠宰豬扁桃腺及豬丹毒解剖病例,進行豬丹毒桿菌之分離調查及血清型鑑定。養豬場所收集之244糞材樣本中有22(9.02%)樣本可分離到豬丹毒桿菌。其中以春季(3-5月)之27.62%及夏季(6-8月)之10.22%分離率最高,且明顯高於其他月份。此外,在這六個月中現場發生豬丹毒疫情亦有較多之趨勢。另由八、九月間所採集150屠宰豬扁桃腺樣本中,也有21.3%(32/150)之分離率,顯示臺灣春夏二季為好發豬丹毒之季節。
  由114分離株中進行血清型鑑定,除18株未能鑑定外,其餘分屬於1a、1b、2、4、6、12、20、七種血清型。其中1a、1b、及2型血清型佔74.6%是為本省豬丹毒桿菌主要備清型。在毒性鑑定上有77/114(67.54%)分離株對豬具弱毒性,其中93.62%(44/47)之1a型及72%(18/25)之2型菌對豬具強毒性,其他血清型則不具或僅具弱毒性而已。由解剖病例所分離之47株中,經血清型鑑定僅見1a及2二型,顯見是為引起本省臨床上豬丹毒之主要二種血清型。
  由豬場糞材及屠宰鍤扁桃腺之分離株。血清型鑑定顯示2型菌之分離率甚高,分別佔17.4%(4/23)及45.2%(19/42)。而且2型菌在毒性試驗亦顯示對豬具毒性,顯見2型菌在疾病之發生方面有其重要地位。
  在純明膠平皿菌落生長型態觀察上,主要有毛球狀、樹根狀、及密枝狀三種生長型態。1型菌中呈毛球狀乾不具毒性,樹根狀具有較高毒性則次之。在2型菌中三種型態菌落對豬均具毒性,惟毛球狀菌落毒力較低,樹根狀居中,密枝狀毒力最高。故菌落生長型態可為鑑定菌株毒力之指標。
  藥物感受性試驗中顯示所有分離株對Penicilin系抗生素均具高感受性 ,而以Ampicilin之感受性最高。絕大部份分離株對0TC(91%)、CTC(99%)及Chloramphenicol(77%)具有高耐受性,惟菌苗株卻仍具感受性。
  交叉保護試驗顯示經二劑量1a型菌茵免疫之小白鼠對盯同血清型強株或弱毒株之攻擊具完整保護性,但一劑量免疫之小白鼠則對相同血清型強毒株之攻擊后,仍有關節炎,顯示僅具不完全保護。惟無論一劑量或二劑量1a型菌苗免疫之小白鼠對不同血清型之攻擊雖具交叉保護性,但不完全保護。
  Several samples, including the feces collected from pig farm, the tonsils of slaughtered pigs and the necropsied pigs infected with erysipelas, were collected for isolation and serotyping of Erysipelothrix rhusiopathiae from March 1992 through February 1993.
  In the farms, the isolation rate was 9.02% (22/244), and mostly distributed in spring (27.62%) and summer (10.22%) seasons. In slaughtered pigs, the isolation rate of the tonsils was 21.3% (32/150) which were sampled in the period of August and September, the more popular seasons present the occurance of swine erysipelas.
  A total of 114 isolates were identified their serotypes as type 1a, 1b, 2, 4, 6, 12 or 20, except 18 isolates were untypable strains. Among them 74.6% belongs to type 1a, 1bor2.Results of the toxcity test showed that 67.5% (77/114) of the isolated strains were toxicto the pig,of these, 93.62% (44/47) type 1a and 72% (18/25) type 2 were the high virulent strains. All the 47 strains isolated from necropsied cases were identified as high virulent type 1a or 2.
  The strains identified as serotype 2 had higher isolation rates either in pig farm (17.4%) or in slaughtered pigs (45.2%). These type 2 strains were proven toxic and might also be associated with the disease in pig farms.
  Three types of colonies as globoid, rhizoid and dense ramiform were observed. In type 1, the globoid type of colony was proven to be avirulent. The other two types were virulent, the rhizoid type was more toxic than the dense ramiform. On the contrary, these 3 types were proven to be toxic in type 2. The virulent abilities were demonstrated as dense ramiform >rhizoid >globoid. The results indicated that the colonied types of pure gelatin plate cultre can be a indicator to indentify the virulence of E. rhusiopathiae strains.
  The drug sensitivity tests showed all the isolates were sensitve to penicillin series antibiotics. Among them. Ampicillin was the most sensitive one. Most of the isolated strains had more or less resistent to Oxytetracyclin, Chlortetracyclin and Chloramphenicol, but the vaccine strains still showed sensitive to them.
  Tests of cross immunity had been done in this study. The mice vaccinated by type 1a living bacterin double doses had a complete protection against high or low virulent type 1a strain challenged, but incomplete protection was shown in the mice vaccinated by only one dose. Arthritis was visible in this incomplete protection mice. No matter vaccinated with one or double doses of the 1a living bacterin , incomplete protection was shown after challenged by various serotypes other than type 1a.
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