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研究生:蘇珍珮
研究生(外文):Su, Jen-Pey
論文名稱:小鼠小腸內壁上皮細胞間隙淋巴細胞之定性:T細胞接受器β鏈可變區域之使用分析及抗T細胞抗體引起之反應分析
論文名稱(外文):Characterization of Murine iIEL subpopulation: analysis of TCR Vβ expression and anti-TCR antibody induced response
指導教授:賴明宗賴明宗引用關係
指導教授(外文):Lai, Ming-Zong
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:微生物學系
學門:生命科學學門
學類:微生物學類
論文種類:學術論文
論文出版年:1993
畢業學年度:81
語文別:中文
論文頁數:70
中文關鍵詞:小腸內壁上皮細胞間隙淋巴細胞細胞分化群標誌8T細胞接受器αβ
外文關鍵詞:iIELCD8TCR-αβ
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  小腸內壁上皮細胞間隙中散佈著許多淋巴細胞,稱為 intestinal
intra epithetial lymphocyte (iIEL)。由已有的報告中顯示此群細胞之
組成複雜。在細細胞表型、成熟分化及免疫功能上均與週邊淋巴細胞有很
大的差異。本研究將 iIEL 依其 CD4 、CD8α 鏈及 CD8β 鏈之表現區分
為 CD4 單陽性(single positive, SP),CD4+ CD8α+ 雙陽性 (double
positive, DP),αβ CD8+ 及 αα CD8+ 幾個亞群,以螢光染色法分析
各亞群細胞在第二型 T 細胞接受器β鏈可變區域 (TCR Vβ) 之使用,未
見特殊 TCRV β使用之好惡。分析 Mls-1a 老鼠中 Vβ6+ iIEL 來探討可
能存在之負面選擇(negative selection)。發現αα CD8 iIEL 之 Vβ6+
細胞仍存在。分析 H-2k 老鼠中 Vβ14+ CD8+ iIEL,探討其正面選擇之
可能性(positive selection)發現不同於週邊 T 細胞。而由分析 CD44
Ly- 6C等分子之表現,可看出 iIEL 與週邊淋巴細胞之不同不僅表現在
αα CD8 iIEL 上,CD44 SP iIEL 及 αβ CD8 iIEL 亦有明顯之不同。
探討各亞群細胞在抗 TCR Cβ 抗體刺激下之增殖反應,顯示較週邊淋巴
細胞低很多,尤其是 αα CD8 iIEL 即使在外加 IL-2 下多未見增殖。
而分析刺激後 IL-2, IL-4 及 IFN-γ 之分泌能力,顯示 CD4 SP iIEL,
αβ CD8 iIEL 均可生成 IL-2, IFN-γ,但 αα CD8 iIEL 則仍未測到
。而 IL-4之分泌並未能在任一亞群細胞中偵測到。由本研究結果再次顯
示 iIEL 與週邊淋巴細胞之不同。且在不同的亞群,其反應、功能亦不盡
相同。

Recent studies have suggested that intestinal intraepithelial
lymphocytes (iIEL) iIELs are quite different from peripheral T
cells in various aspects of their development and functions. By
characterizing iIEL subpopulations according to their surface
expression of CD4, CD8a, and CD8b chains, we analyzed ab T cell
receptor (TCR) Vb chain usage by flow cytometry, and found
that, unlike in human iIEL, there is no preferential usage of
Vb in murine iIEL. Within the aaCD8 abTCR subpopulation, Vb6+
cells was remained in Mls-1a mice. Similar frequency of Vb14+
CD8+ iIEL was found in Kk mice as in Kb mice. It suggests that
selection of abTCR in aaCD8 iIEL during their maturation is
different from that of peripheral T cells. Analysis of CD44
and Ly6C expression revealed that not only aaCD8 but abCD8 and
CD4 iIEL expressed differently from that of peripheral CD4 and
CD8 cells. We further characterize iIEL subpopulations by
examining their proliferation activity and cytokine production
in response to stimulation via abTCR with or without
costimulation of CD28 or CD2. We found that CD4 and abCD8 iIEL
proliferate in the presence of exogenous IL2, whereas aaCD8
iIEL showed no proliferative response. The same response
pattern was observed for IL2 and IFN-r production after
stimulation, even under the condition without exogenous IL2 and
no detectable proliferation response. None of the iIEL
subpopulations nor lymph node CD8 cells produce IL4 in response
to the above stimuli. The present study shows that CD4, abCD8,
and aaCD8 iIEL are quite distinct from peripheral T cells in
surface marker and functions. Different iIEL subsets may have
different function. However, how the unique properties of iIEL
relate to their functions in vivo requires further
investigation.

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