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研究生:馬秀華
研究生(外文):Ma,Shiow-Hwa
論文名稱:感染中華肝吸蟲大白鼠膽管之病理及免疫組織化學研究
論文名稱(外文):Pathological and Immunohistochemical Studies on the Bile Ducts
指導教授:蘇霩靄
指導教授(外文):Su,Kua-eyre
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:微生物學系
學門:生命科學學門
學類:微生物學類
論文種類:學術論文
論文出版年:1993
畢業學年度:81
語文別:中文
論文頁數:102
中文關鍵詞:中華肝吸蟲.大白鼠.病理學.免疫組織化學.
外文關鍵詞:Clonorchis sinensis. Rat. Pathology. Immunohistochemistry.
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本實驗主要為觀察大白鼠感染中華肝吸蟲後,膽管及肝臟所發生組織病理
學變化之演變過程。今將三十六隻大白鼠,每隻經口感染五十個吸蟲囊蚴
。分別於三天、一、二、四、六、八、十、十二、十四、十六、二十及三
十週時,犧牲大白鼠,取出肝臟,切片觀察之。結果顯示,感染一週後蟲
體出現於膽管之中。在肝臟實質部份,僅發現一些發炎細胞浸潤及肝內膽
管增生的現象,並未出現嚴重病變。四至十週時,膽管中蟲體數量最多,
之後蟲體數量逐漸減少。最明顯的病理變化發生在肝外膽管(肝管及總膽
管)及較大的膽管分支。三天至二週期間,屬急性發炎期,管壁出現大量
的嗜酸性及嗜中性白血球。上皮細胞分裂、增生,偽複層及腺體包封現象
出現。感染後四∼十週,漿細胞大量出現。蟲體已成熟,充塞於管腔之中
,使管壁逐漸擴張。上皮細胞分裂、增生加劇,偽複層更加明顯,腺瘤樣
組織出現。杯狀細胞增生,黏液分泌增加。管壁出現輕微纖維組織。感染
後十二∼十六週,蟲數下降,膽管依然持續擴張。膽汁嚴重淤積管中。淋
巴細胞數量增多。膽管周圍纖維化加重。腺瘤樣增生使蟲體被局限於較小
的空間。腺體增生趨緩,宿主膽管粘膜層慢慢恢復正常。至感染二十∼三
十週,蟲體已不復見。管壁除少數淋巴細胞及組織球外,均嚴重纖維化。
粘膜層恢復平坦,上皮細胞發生扁平化生。免疫染色結果,管壁肌肉層漿
細胞以分泌免疫球蛋白G與免疫球蛋白E為主。而免疫球蛋白M與免疫球
蛋白A的量甚微。

The major purpose of this study is to observe what the
microscopic pathologic changes of the bile duct and liver of
the white rats were after they were infected with Clonorchis
sinensis. Now we let rats be infected with 50 metacercaria by
oral route each.Then at the third day to the thirtieth weeks,
respectively, we sacrificied these white rats, took out their
liver and made biopsy of them,then observed them. The results
showed that during the fourth week to the tenth week, the most
parasites were found in the biliary tracts, then the amount
began to decrease gradually. The most obvious pathologic changs
happened in the hepatic ducts, commom bile ducts and those
larger branch of bile ducts. During the third day to the second
week,it was belonged to acute stage, and there were mich
eosinophils and neutrophils were found in the wall of bile
ducts.The epithelium began to differentiate and proliferate.
The enveloping phenomena of glands showed up. During the fourth
week to the tenth week,large amount of plasma cells were found.
The parasites growed in maturity, occupied the whole lumen and
made the lumen to dilate larger and larger. Mean while, goblet
cells proliferated and secretion of mucin increased.Little
fiber tissue were found in walls of lumens. During the twelfth
week to sixteenth week,the number of parasites began to
decrease, and the lumens of biliary ducts still persisted to
dilate. The bile was stasis in the lumens severely. The amount
of lymphocytesadenoid proliferation made these parasites to be
limited in a small space. As the proliferation of the glands
ahowed dowm, th biliary tracts of hostd became normal
pradually. Till the twenteith week to the thirtieth week, there
was not any parasites to be found. The results of the
immunohistochemical staining found the plasma cells in the
muscle wall of the bile ducts secret large mount IgGs and IgEs,
only a little IgM and IgA were found.

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