臺灣博碩士論文加值系統

台灣啤酒因季節因素而有產銷失衡的問題存在, 解決之道在於減少啤酒在
貯存過程中品質之劣變, 以淡季產量來調節旺季所需。啤酒之品質劣變主
要為發生混濁與風味的改變。本研究嘗試添加抗氧化劑及其它添加劑來改
善啤酒之貯存安定性, 發現使用抗氧化劑有助於改善啤酒之貯存, 而使用
上以茶抗氧化劑, 維生素C , 維生素E 及beta環狀糊精等添加劑為佳 ,
對於貯存中啤酒澄清度的維持具有特別的效果。啤酒花為賦與啤酒風味之
重要原料, 為製造啤酒時不可或缺的原料,國內需求全賴進口, 然而在品
管上卻苦於無標準品作為評估的指標, 因此完全仰賴國外檢驗單位代檢,
造成作業上之障礙。為了解決此一問題, 本研究乃針對啤酒花之重要成
份 humulones及 lupulones等進行分離及純化方法之探討, 期能自行分離
純化出檢驗所需的標準品, 以解決國內品管問題及確保啤酒的品質。本研
究發現, 將啤酒花以正己烷萃取, 萃取液經濃縮, 後以冰冷甲醇轉溶, 將
可溶部份通過HP-20 樹脂管柱, 以甲醇溶洗。將甲醇溶洗部份濃縮, 以正
己烷溶解, 然後以pH=9.5的矽膠管柱進行層析 , 以正己烷及氯仿依序溶
洗而分別得到 beta酸及 alpah 酸。將beta酸以正己烷反覆進行結晶而得
beta酸針狀結晶, 純度95%(colupulone87% ,n-, ad-lupulone 8%), 收
率0.17%, 利用此結晶作為外標準品進行定量, 結果發現與使用傳統的啤
酒花抽出物二級標準品進行定量時之對應係數相近。使用此結晶品當作一
級標準品進行定量時, 顯然優於使用傳統之二級標準品。

The sale and production of Taiwan beer is unbalanceable for
wheather reason, Beer plants should produce day and night for
customer need in boom season;but in slack season beer has to be
store for a long time. The stored beer would cause cloudy and
stale flavor during storage, so it is important to improve beer
quality during storage. In this study it was found that the
quality of stored beer can be significantly improved by the
addition of antioxidants. From the view point of food safty, it
is preferred to use natural antioxidants such as tea
antioxidant , Vit C, Vit E and βcyclodextrin. Hop is an
important raw material in beer production. But hop supplies in
Taiwan for beer producing are all relying on import. Without
analytical standards,we ensure hop quality by relying on
inspection organizations. Therefore, it is necessary to search
for standard for hop quality control.In the study of hop
compon- ents, it was found that extracting bitter acids by n-
hexane is better than using other solvents. We can get best
bitter acids extraction efficiency by using n-hexane to extract
for 1 hour, and repeating twices. Then,we change cold methanol
to dissolve hop condense for separate nonpolar material.When
methanol soluble take HP-20 column chromatography, we could
separate bitter acids mixture from methanol fraction and
pigment mixture from acetone fraction.Continueally,it is found
that β-acids and α-acids can be effectively separated by
modificied silica gel(pH=9.5) column chromatograhy with n-
hexane, chloroform as the elution system. Then, We can get β-
acids crystal by using n-hexane crystallizd repeatly.The
crystal purity is 95% (87% colupulone, 8% n-,ad- lupulone),
yield is 0.17%. We compare this β-acids crystal with
traditional hop extract standard as analytical standards, then
we find the correspondence factor of internal standard of them
are