臺灣博碩士論文加值系統

本論文的目的是為了發展一種不需採樣破壞土壤而能直接偵測脫氮作用的
技術，所以欲利用壓電晶體氣體生物感測器建立一種快速分析脫氮作用之
方法。本論文採用9 MHZ 的壓電晶體，氣體分析方式是利用注射針取定體
積之樣品注入密閉的偵測瓶中與晶體反應。所嚐試的覆被物質中，無論是
化學物或生物性物質皆會受氣體中所含水氣之干擾，所以在分析過程將相
對濕度控制在20% 左右；其中以Thiosphaera pantotropha細胞膜覆被之
晶體對一氧化二氮反應最好，並符合 Langmuir方程式，與一氧化二氮濃
度範圍0.1∼20ppm之間成線性關係，靈敏度可達　0.1ppm。T.
pantotropha 細胞膜的覆被量與偵測範圍、靈敏度及反應時間有關，以覆
被量較大者(107.14 .sim.142.86.mu.g) 反應較明顯，比較符合於實際運
用。

In order to develope a non-destructive technique to detect
denitrification in soils, the piezoelectric quartz gas
biosensor is taken a selected method here to detect gaseous
intermediate metabolites of denitri- fication. The purpose of
this studies was to establish a method that could analyze
rapidly. The 9 MHZ quartz crystals were used in this study ,
and sealed injection method was applied to analyze gaseous
intermediate metabolites of denitrification. The application of
various chemically and biologically coated crystals were
tested, and interference caused by the humidity of gas, so
the relative humidity of sealed bottle was controlled at 20%
during the mea- surement. The results indicated that the
crystals coated with Thiosphaera pantotropha membrane showed
better response than the others examined. And the data could
fit the Langmuir equation. A linear cali- bration over the
range of 0.1∼20 ppm of nitrous oxide was obtained and
the detection limit of the technique was determined to be 0.1
ppm . The amount of coating with T. pantotropha membrane
would effect the detecting range, response frequency and
response time. The coating with 107.14.sim.42.86 .mu.g on
crystal was very effective for practical applications.