(3.238.186.43) 您好!臺灣時間:2021/02/25 01:31
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

我願授權國圖
: 
twitterline
研究生:陳俊任
研究生(外文):Chen,Chun-Jen
論文名稱:Aeromonassp.No.16所生產幾丁質分解酵素之研究
論文名稱(外文):Studies on Chitinolytic Enzymes from Aeromonas sp. No. 16
指導教授:蘇遠志;黃健雄
指導教授(外文):Su,Yuan-Chi;Hiang,Jan-Hsiung
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:農業化學系
學門:農業科學學門
學類:農業化學類
論文種類:學術論文
論文出版年:1993
畢業學年度:81
語文別:中文
論文頁數:152
中文關鍵詞:嗜水性需氣單胞菌幾丁質N-乙醯幾丁寡醣
外文關鍵詞:AeromonasChitinaseN-Acetylchitooligosaccharide
相關次數:
  • 被引用被引用:4
  • 點閱點閱:76
  • 評分評分:系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
幾丁質及幾丁聚醣經部份水解後之產物-N-乙醯幾丁寡醣及幾丁寡醣在各
領域之應用已逐漸受到重視。本研究擬利用微生物酵素來進行此類寡醣之
生產,以期將蟹蝦殼等水產廢棄物轉變成為高附加價值之產品。將本地水
產加工過程中所產生之蟹殼經酸鹼處理後可得到蟹殼幾丁質。以此作為醱
酵基質,由自然界篩選出103株可分解幾丁質之菌株,其中以編號 16之菌
株具有生產多量幾丁質分解酵素之潛力。根據菌種鑑定結果判斷其歸屬
於 Aeromonas 菌屬,但種名尚未確定,故暫命名為 Aeromonas sp.
No. 16。 Aeromonas sp. No. 16 於 500 mL Hinton 氏三角瓶中生產幾
丁質時,當培養基中含有 1.5%膠態幾丁質,2.0% tryptone, 1.5%
yeast extract及培養起始pH 10之情況下,於30℃,100 rpm 振盪培養24
小時,其酵素產量可達1.4 單位/毫升;於5公升醱酵槽中以結晶態幾丁質
為醱酵基質進行培養時,在 30℃,攪拌速度400 rpm,通氣量1 vvm及 pH
控制在 7∼8之條件下,培養30小時可使酵素產量達 1.5 單位/毫升。幾
丁質與N-乙醯葡萄糖胺皆可誘導Aeromonas sp. No. 16合成幾丁質,其
他醣類之添加則會對酵量生產造成不同程度之抑制作用。粗酵素水解幾丁
質之主要產物為N-乙醯葡萄糖胺及少量二醣,水解幾丁聚醣則獲得未知產
物,推測其為異幾丁寡醣 (hetero-chitooligosacc- haride)。粗酵素中
幾丁質活性之最適作用pH值及溫度分別為6.0及 50℃,在pH 5∼9及溫
度低於 40℃ 時較為安定。β-N-乙醯葡萄糖胺之熱安定性較差,推測
可能為細胞內酵素。粗酵素經PAGE及活性染色後發現有數種蛋白質具有幾
丁質活性,經硫酸銨分劃及製備式聚丙烯醯胺膠體電泳後所得之純化酵
素於 SDS-PAGE 顯示有三條明顯之蛋白質色帶,分子量分別為 101 KD
,92 KD及 56 KD。純化之酵素分解膠態幾丁質之主要產物為 N,N''''-二乙
醯幾丁二醣及少量單醣、三醣及四醣。
The partially hydrolyzed products of chitin and chitosan, N-
acetylchitooligosaccharides and chitooligosaccharides, are of
special interest because these oligosaccharides have been
evalu- ated in various applications.This study focused on the
production of N-acetylchitooligosaccharides by microbial
enzymes. Crab shell chitin prepared by treating crab shell
with acid and alkali was used as a substrate for isolating
chitinolytic mi- croorganisms. Among the 103 isolated
strains, strain No. 16 appeared to be the most potential
chitinase-producing strain. It was identified belonging to the
genus Aeromonas and named Aero- monas sp. No. 16. When the
medium contained 1.5% colloidal chitin , 2.0% tryp- tone and
1.5% yeast extract with initial pH 10, Aeromonas sp. No. 16
could produce 1.4 units/milliliter of chitinase activity after
24 hours cultivation in 500 mL Hinton''''s flask. Using crys-
talline chitin as substrate for enzyme productionin a 5L
fermen- tor with temperature at 30℃, aeration at 1 vvm,
agitation at 400 rpm and pH controlled between 7 and 8,
chitinase activity could reach 1.5 units/milliliter after 30
hours cultivation. Chitin and N-acetylglucosamine were both
found to be inducers for chitinase synthesis by Aeromonas sp.
No. 16, on the other hand, addition of other carbohydrates
would repress enzyme production with different degrees.
Crude enzyme hydrolyzed chitin and produced N-acetylglucosa-
mine as main product ,hydrolyzed chitosan with unknown
products formation, which was supposed to be hetero-
chitooligosaccharide. In crude enzyme, chitinase activity was
most active at pH 6.0 and 50℃, and was stable at pH between 5
and 9 and at temperature be- low 40℃. β-N-acetyl-D-
glucosaminidase was less stable at 40℃ and supposed to be an
intracellular enzyme. After PAGE and acti-
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
無相關期刊
 
系統版面圖檔 系統版面圖檔