臺灣博碩士論文加值系統

用 S-hexylglutathione 吸附性管膠與陰離子交換管膠Mono Q可將斑節蝦
眼睛的穀胱甘■轉換■θ和穀胱甘■轉換■μ分離。比較穀胱甘■轉換■
θ與穀胱甘■轉換■μ的相對含量以眼睛和鰓最高，神經次之。根據硫酸
十二酯鈉聚丙烯醯胺膠體電泳、Superose-12 的証據，顯示穀胱甘■轉換
■θ為二聚體（Dimer ），分子量約為 50 KDa ，能與皮質酮結合。經硫
酸十二酯鈉聚丙烯醯胺膠體電泳分析可結合在單元體上，並能以 10mM 的
穀胱甘■影響穀胱甘■轉換■θ與皮質酮間的結合。老鼠的蛋白質激■ C
（PKC ）可使斑節蝦穀胱甘■轉換■θ進行磷酸化，但是，斑節蝦的蛋白
質激■C 無法使斑節蝦的穀胱甘■轉換■θ 磷酸化。

Two anionic isoenzymes of glutathione S-transferase (EC
2.5.1.18 ), GST θ and GST μ, have been purified from the eyes
of the shrimp Penaseus japonicus by using a combination of S-
hexyl- glutathione affinity column chromatography and Mono Q
fast protein liquid chromatography (f.p.l.c.). The purified
GST θ was a dimeric enzyme protein with subunit Mr abount 25
kDa. HPLC reverse phase C4 column analysis of various shrimp
tissues indicated that the ratio of GST θ vs. GST μ was
highest in the eye and gill. HPLC reverse phase C4 column
analysis demostrated that corticosterone was selective binding
to GST θ. Glutathione influenced the binding of corticosterone
to the GST θ.