跳到主要內容

臺灣博碩士論文加值系統

(44.201.97.224) 您好!臺灣時間:2024/04/14 18:08
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:洪毓春
論文名稱:番荔枝果實之化學成分與生物活性之研究
論文名稱(外文):Studies on the Fruits of Annona squamosa-Chemical Constituents and Biological Activities
指導教授:吳永昌
學位類別:碩士
校院名稱:高雄醫學大學
系所名稱:天然藥物研究所
學門:醫藥衛生學門
學類:藥學學類
論文種類:學術論文
論文出版年:1994
畢業學年度:82
語文別:中文
論文頁數:158
中文關鍵詞:蕃荔枝生物活性
相關次數:
  • 被引用被引用:4
  • 點閱點閱:216
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
番荔枝(Annona squamosa)俗稱釋迦,屬於番荔枝科(Annonaceae)植物,以其未成熟新鮮果食為材料,依生物活性導引,探討該植物之化學成分及其生物活性,分離得到十四種kaurane diterpenoids 化合物: (-)-kaur-16-en-19-oicacid (1)、16β, 17-dihydroxy-(-)-kauran-19-oic acid (2)、16β-(-)-kauran-16,17,19-triol (3)、mixture of 17-hydroxy-16β-(-)-kauran-19-oic acid and 17-hydroxy-16α-(-)-kauran-19-oic acid (4)/(5)、(-)-kaur-16-en-19-ol(6)、16α ,17-dihydroxy-(-)-kauran-19-oic acid (7)、17-acetoxy-16β-(-)-kauran-19-oic acid (8)、(-)-kauran-19-al-17-oic acid (9)、16β、17-dihydroxy-(-)-kauran-19-al (10)、 17-hydroxy-16/5-(-)-kauran-19-al (11)-16β-hydroxy-17-acetoxy-(-)-kauran-19-al (12)- 19-nor-(-)-kauran-4α-ol-17-oic acid (13) and 19-nor-(-)-kauran-4α,16β,17-triol (14)@。其中化合物16β-hydroxy-17-acetoxy-(-)-kauran-19-al (12) 及19-nor-(-)-kauran-4α,16β,17-triol(14)為新化合物且化合物2、3、4/5、7、8、9和10為第一次由番荔枝{A.squamosa)分離得到。而所有化合物之構造式皆由光譜及化學方法加以確定。
化合物1∼14及其衍生物經抗癌及抗人體免疫病毒活性測試後,在抗癌活性方面發現16β,17-dihydroxy-(-)-kauran-19-oic acid (2)對P-388及A-549之癌細胞具明顯的抑制效果,其ED50分別為0.41及0.13 μg/ml。化合物1、2及3對人體免疫病毒(HIV replication)有抑制作用,其中以16β,17-dihydroxy-(-)-kauran-19-oic acid (2)之抑制效果最好,其ED50為0.8μg/ml而所有之化合物對於人體免疫病毒反轉錄酉每(HIV-RT)試驗無明顯之抑制效果。
As a result of our continuing search for novel plant bioactive agents. The methanolic extracts of the fresh fruits of Annona squamosa (Annonaecae), was found to show significant cytotoxicity against in vitro tissue culture cells in human A-549 lung carcinoma, murine P-388 lymphocytic and HIV replication in H9 lymphocyte cells.
Bioactivity-guided chromatographic fractionation led to the isolation and characterization of fourteen kaurane diterpenoids: (-)-kaur-16-en-19-oic acid (1), 16β, 17-dihydrxoy-(-)-kauran-19-oic acid (2), 16β(-)-kauran-16, 17, 19-triol (3), mixture of 17-hydroxy-16β-(-)-(-)-kauran-19-oic acid and 17-hydroxy-16α-(-)-kauran-19-oic acid (4)/(5), (-)-kaur-16-en-19-ol (6), 16α, 17-dihydroxy-(-)-kauran-19-oic acid (7), 17-acetoxy-16β-(-)-kauran-19-oic acid (8), (-)-kauran-19-al-17-oic acid (9), 16β, 17-dihydroxy-(-)-kauran-19-al (10), 17-hydroxy-16β-(-)-kauran-19-al (11), 16β-hydroxy-17-acetoxy-(-)-kauran-19-al (12), 19-nor-(-)-kauran-4α-ol-17-oic acid (13), 19-nor-(-)-kauran-4α, 16β, 17-triol (14). Among them, 16β-hydroxy-17-acetoxy-(-)-kauran-19-al (12) and 19-nor-(-)-kauran-4α, 16β, 17-triol (14) are new compounds and compounds 2, 3, 4/5, 7, 8, 9 and 10 are isolated for the first time from Annona squamsoa. The structural elucidation of the isolates were established by spectroscopic and chemical evidences.
Compound 2 showed significant cytotoxicity against P-388 and A-549 cancer cells with ED50 values of 0.41 and 0.13μg/ml, respectively. Compounds 1, 2 and 3 inhibited HIV replication in H9 lymphocyte cells. Especially, compound 2 showed potent anti-HIV activity in H9 lymphocyte cells with an ED50 of 0.8μg/ml. All of the compounds did not show significant effective inhibition against human immunodeficiency virus reverse transcriptase (HIV-RT).
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top