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研究生:李欲禎
研究生(外文):Lee, Yu-Chen
論文名稱:機械性應力對人類牙周韌帶造纖維細胞影響的探討
論文名稱(外文):The Effects of Mechanical Stress on Human Periodontal Ligament Fibroblasts
指導教授:張宏博
指導教授(外文):Chang, Hong-Po
學位類別:碩士
校院名稱:高雄醫學大學
系所名稱:牙醫學研究所
學門:醫藥衛生學門
學類:牙醫學類
論文種類:學術論文
論文出版年:1994
畢業學年度:82
語文別:中文
論文頁數:76
中文關鍵詞:機械性應力牙周韌帶纖維細胞
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在齒顎矯正治療過程中,對牙周組織施與矯正力後,牙周組織的變化,在先前多位學者利用組織學或生物化學測定等方法已有多項研究。本實驗嘗試將人類牙周韌帶中數量最多,且與施力作用最有相關的造纖維細胞,利用細胞培養技術獨立培養,並在活體外模擬矯正施力,設計一施力模板,以約100-150克重的力(20%伸張百分比),對細胞分別施以連續性及間歇性伸張力,作用三天,再比較細胞對此應力在細胞數目、增殖速率(MTT Kit)及分化程度(鹼性磷酸酉每活性)上的變化。
根據本實驗結果顯示:1)機械性伸張力不論是連續性或間歇性施力,在對牙周韌帶造纖維細胞作用的最初三天,皆會使細胞數目及細胞增殖速率較對照組來得高;而且間歇性施力的效應略大於連續性施力的效應;2)牙周韌帶中的造纖維細胞確實具有類造骨細胞的特質,不過其鹼性磷酸酉每活性的個體差異極大;且其活性不論施力與否,在細胞生長期都會隨著時間而升高,直到融合期達最高峰;3)機械性伸張力對細胞分化程度的影響,一直到作用48小時後才出現,所以可能是一種延遲效應;4)在細胞分化程度的影響上,連續性施力與間歇性施力並無差異,顯示細胞分化程度的變化似乎與施力型態無關,只與施力與否有相關。
所以,在活體外培養人類牙周韌帶造纖維細胞的實驗中,機械性應力確實是影響細胞增生及分化功能的重要因子。

The sequential reaction of periodontal tissue after application of orthodontic forces has been widely studied by the use of histologic or biochemical methods. In this study, we tried to cultue the human periodontal ligament fibroblasts (HPLF), and designed a template for mechanical stretch application (about 20% stretch percentage). Cells were subjected to continuous or intermittent force (1 cycle a day) for 3 days and responded as evaluated by cell number counting, cell proliferation rate and alkaline phosphatase (ALPase) activity.
These results revealed that : (1) The cell number and cell proliferation rate increased in continuous and intermittent force groups as compared with control groups; and the intermittent effect was greater than continuous. (2) The ALPase activity was detected in HPLF, and it is suggested that HPLF has osteoblast-like property. The variation of ALPase activity was wide among individuals, and the activity increased with time, until they grows confluence. (3) Stressed HPLF in culture did not increase the ALPase activity until at the second day, it seems that the effects on cell differentiation level may be a delayed response. (4) The effects of mechanical stretch on HPLF ALPase activity were the same between continuous and intermittent forces. While speculative, the cell differentiation level does not relate to the stretch pattern, just to the existence of stress.
These findings suggest that mechanical stress in vitro may regulate the functions of cell proliferation and differentiation of human periodontal ligament fibroblasts (HPLF).

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