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研究生:吳安娜
研究生(外文):Anna Wu
論文名稱:蔗糖、光度和添加二氧化碳對玫瑰枝梢培植體生長的影響
論文名稱(外文):The effects of sucrose、 PPF and CO2 enrichment on the growth of rose shoot explants.
指導教授:朱建鏞
指導教授(外文):Chein-Young Chu
學位類別:碩士
校院名稱:國立中興大學
系所名稱:園藝學系
學門:農業科學學門
學類:園藝學類
論文種類:學術論文
論文出版年:1994
畢業學年度:82
語文別:中文
論文頁數:80
中文關鍵詞:蔗糖光度添加二氧化碳玫瑰花微體繁殖
外文關鍵詞:sucrosePPFCO2 enrichmentrosemicropropagation
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本研究採用''Samantha''、''Landora''和''Pitica''三個臺灣主要切花品種的
繼代培植體為試驗材料,探討微體繁殖過程中,培養基的蔗糖濃度、培養
光度以及添加CO2的處理,對於枝梢培植體生長的影響。培養於培養基蔗
糖濃度分別為3.8g/L、7.5g/L、15g/L和30g/L的枝梢培植體,''Samantha''
和''Landora''的生長量,隨培養基中蔗糖濃度增加而增加;''Pitica''則以
培養於蔗糖濃度15g/L的培養基的培植體生長最好。三品種的枝梢培植體
增殖率,隨蔗糖濃度增加而增加。培養容器內的CO2濃度的變化,隨培養
的光週期而改變,明期開始2∼ 4小時後達到一低濃度的平衡點;而當暗
期開始後約4小時,CO2濃度會累積至一高濃度的平衡點。在高糖培養基中
培養玫瑰枝梢,無論在明期或暗期,容器內CO2濃度的平衡點均高於低糖
培養基培養者。 ''Samantha''和''Landora''的枝梢培植體,在高光度(70.mu.
mole/m2/sec)下培養,其生長量均大於低光度(35.mu.mole/m2/sec)下培養
者;又在高光度下,且在蔗糖濃度為15 g/L培養基中培養者,生長量較培
養於蔗糖濃度為30 g/L培養基者高。而''Samantha''在高光度、無糖的培養
基中可自營生長,但存活率僅約40﹪;但在相同的條件下''Landora''則無
法存活。另外在低光度下、於無糖培養基中,培植體於培養兩週後死亡。
在CO2濃度的變化方面,在高光強度下培養四週後,明期時容器內的CO2濃
度的平衡點,以蔗糖濃度15g/L培養基者最低,顯示培植體光合能力最強
。在高光強度下,明期時容器內添加CO2處理,對培植體之鮮重無顯著差
異,但對''Samantha''和''Landora''培植體則有利於乾物的累積,且葉色較
為濃綠。經添加CO2處理後,培養在蔗糖濃度為7.5g/L之培養基的培植體
,其生長勢最強;而在蔗糖濃度為15 g/L時,''Samantha''的培植體葉片扭
曲變形,而''Landora''下位葉黃化的現象明顯。在高光度下培養的培植體
,其氣孔呈橢圓形,開張度不一致,而其葉肉組織有明顯的柵狀組織;而
配合添加CO2的處理後,氣孔呈橢圓形,開張度小或甚至關閉,葉肉組織
的柵狀組織明顯呈長柱狀,且葉片厚度明顯大於無CO2處理者。另外
''Landora''的培植體,在高光度下發育的葉綠體,有明顯澱粉粒產生,配
合添加CO2處理,且培養於蔗糖濃度為7.5g/L培養基者,其葉綠體內的澱
粉粒最大。
Effects of sucrose concentration,photosynthetic photon flux(
PPF) and CO2 enrichment on the growth of shoot explants of
''Samantha'',''Landora'' and ''Pitica'' roses were studiedin vitro.
The level of sucrose in medium was at 3.8、7.5、15 or 30g/L.The
more growth of ''Samantha'' and ''Landora'' explants was cultured
on the medium contained higher concentration of sucrose.
However, the best growth of ''Pitica'' explants was cultures on
the medium contained 15g/L sucrose. And the multiplication rate
of shoot explants increased as the sucrose concentration
increased in medium.The CO2 concentration in vessel was higher
when the sucrose concentration of culturing medium was higher.
The growth of ''Samantha''and ''Landora''explants cultured under
high PPF(70.mu./m2/sec) was greater than that cultured under
low PPF (35.mu.mol/m2/sec).Moreover,under high PPF,the growth
of explants cultured on a medium contained 15g/L sucrose was
greater than that cultured on a medium contained 30g/L sucrose.
Under high PPF,''Landora'' explants did not survive on a medium
without sucrose but 40% of ''Samantha''explants survived.After
cultured under high PPF for 4 weeks, the lowest CO2
concentration in vessel was in which the explants grow on a
medium contained 15g/L sucrose. The dry matter of explants was
higher and the leaves was dark green with CO2 enrichment.
Explants cultured on a medium contained 7.5g/L sucrose and
enriched with CO2 was the vigorest and a medium contained 15g/L
sucrose enriched with CO2, the leaves of ''Samantha'' explants
was distortion and the low leaves of ''Landora'' explants was
yellowing.Under high PPF, on the leaf of explants there were
ellipsoid stomata with different aperture. Furthermore, the
leaf had typical palisade mesophyll and there wrer starch
grains in chloroplast. The additional CO2 enrichment culture,
the aperture of stomata was smaller or closed, and the leaf
thickness was greater and there was the biggest starch grain if
the explants cultured on a medium contained 7.5 g/L.
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