臺灣博碩士論文加值系統

以大頭鰱,草魚, 虱目魚及吳郭魚等四種台灣重要之淡水養殖魚類為實驗
動物，來評估 11 種聚醣類（Bar, curdlan, dextran sulfate, inu-
lin,krestin, laminaran, levan, PO, scleroglucan, yeast glucan及
zymosan）對於這二種魚類細菌，Aeromonas hydrophila 及 Edward-
siella tarda 感染時的保護效力.在存活率試驗中，人工感染
Aeromonas hydrophila 結果顯示有四種聚醣類 (Bar, krestin,
scleroglucan 及 zymosan）能顯著地提高草魚 60 ﹪, 70﹪, 90﹪ 及
60﹪）及吳郭魚（80﹪, 60﹪, 70﹪ 及 60﹪）之存活率P＜0.05)；然而
卻有五種聚醣類(Bar, curdlan, krestin, sc- leroglucan, 及
zymosan) 能顯著地提高大頭鰱及虱目魚的存活率. 若將人工 感染之細菌
改為 Edwardsiella tarda 後, 則結果顯示有四種聚醣類 (Bar,
krestin, scleroglucan, 及 zymosan), 可以顯著提升四種魚之存活率.
由上述之實驗結果挑選五種聚醣類 Bar, curdlan, krestin, sclerogl-
ucan 及 zymosan ，進行 NBT（nitroblue tetrazolium）吸附染色試驗
(Adherence/NBT Assay）藉統計 NBT-陽性細胞數量上升情形，來作為評
估聚醣類對魚體非特異免疫系統的作用。實驗結果顯示，五種聚醣類(除
curdlan 應用於虱目魚時外), 均可顯著提升四種魚之存活率.因此，由本
研究結果顯示，某些聚醣類例如 Bar、krestin、scleroglu- can及
zymosan，在這四種魚類體內具有抗菌之保護效力；減少魚隻感染細菌時
之死亡率；而聚醣類的保護機制，由實驗的結果顯示至少與活化非特異免
疫系統之吞噬細胞作用有關。

The aims of this study are to evaluate the efficacies of 11 gl-
ycans（Bar, curdlan, dextran sulfate, inulin, krestin, laminar-
an, levan, PO, scleroglucan, yeast glucan, and zymosan） to pr-
otect four different species of fish, bighead carp（
Aristichthys nobilis), grass carp (Ctenopharyngodon idellus),
milk fish (Cha- nos chanos), and tilapia (Tilapia aureas P.)
against bacterial infections in vivo. The result revealed that
several glycans, Bar, krestin, sclerog- lucan, and zymosan, did
significantly protect the four kinds of fish against the
bacterial infections. At the same time, the data shown that the
antibacterial mechanism of glycans associat- ed with the
activation of the nonspecific phagocytosis. The results of
challenging with Aeromonas hydrophila study sugg- ested that
four glycans, Bar, krestin, scleroglucan, and zymosa- n,
significantly increased the survival rates in grass carp（6
0﹪, 70﹪, 90﹪, and 60﹪） and tilapia（80﹪, 60﹪, 70﹪, and
60﹪ five glycans, Bar, curdlan, krestin,scleroglucan,and
zymos- an,did significantly increase the survival rates in both
bighead carp（70﹪, 40﹪, 40﹪, 70﹪, and 50﹪） and milk fish
（50﹪, , 40﹪, 60﹪, and 40﹪）. The results of challenging
with Edwardsiella tarda study indicated that four glycans, Bar,
krestin, scleroglucan, and zymosan, did significantly raise the
survival rates of all fishes. Studing on the kinetics of the
numbers of nitroblue tetrazolium (NBT）- positive staining
cells by the Adherence/NBT Assay eva- luates the non-specific
immunity responses in the fish injected with the five glycans
including Bar, curdlan, krestin, sclerogl- ucan, and zymosan,
by the dose of 10mg/kg bw, respectively. The result revealed
that those five glycans, except curdlan in milk fish, did
significantly increase the numbers of NBT- positive staining
cells in the four species of fish.